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Quercetin increases bioavailability of nitric oxide in the jejunum of euglycemic and diabetic rats and induces neuronal plasticity in the myenteric plexus.
Auton Neurosci. 2020 09; 227:102675.AN

Abstract

Considering the antioxidant, neuroprotective, inflammatory and nitric oxide modulatory actions of quercetin, the aim of this study was to test the effect of quercetin administration in drinking water (40 mg/day/rat) on neuronal nitric oxide synthase (nNOS), vasoactive intestinal peptide (VIP), overall population of myenteric neurons (HuC/D) and nitric oxide (NO) levels in the jejunal samples from diabetic rats. Male Wistar rats were distributed into four groups (8 rats per group): euglycemic (E), euglycemic administered with quercetin (E+Q), diabetic (D) and diabetic administered with quercetin (D+Q). Rats were induced to diabetes with streptozotocin (35mg/kg/iv) and, after 120 days, the proximal jejunum were collected and processed for immunohistochemical (VIP, nNOS and HuC/D) and chemiluminescence (quantification of tissue NO levels) techniques. Diabetes mellitus reduced the number of nNOS-IR (immunoreactive) (p <0.05) and HuC/D-IR (p <0.001) neurons, however, promoted an increased morphometric area of nNOS-IR neurons (p <0.001) and VIP-IR varicosities (p <0.05). In D+Q group, neuroplasticity effects were observed on HuC/D-IR neurons, accompanied by a reduction of cell body area of neurons nNOS- and VIP-IR varicosities (p <0.05). The NO levels were increased in the E+Q (p <0.05) and D+Q group (p <0.001) compared to the control group. In conclusion, the results showed that quercetin supplementation increased the bioavailability of NO in the jejunum in euglycemic and mitigate the effects of diabetes on nNOS-IR neurons and VIP-IR varicosities in the myenteric plexus of diabetic rats.

Authors+Show Affiliations

Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, n 5790 Bloco O-33, Maringá, PR CEP 87020-900, Brazil.Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, n 5790 Bloco O-33, Maringá, PR CEP 87020-900, Brazil.Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, n 5790 Bloco O-33, Maringá, PR CEP 87020-900, Brazil.Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, n 5790 Bloco O-33, Maringá, PR CEP 87020-900, Brazil.Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, n 5790 Bloco O-33, Maringá, PR CEP 87020-900, Brazil.Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, n 5790 Bloco O-33, Maringá, PR CEP 87020-900, Brazil.Department of Statistic, Universidade Estadual de Maringá, Avenida Colombo, n 5790, Maringá, PR CEP 87020-900, Brazil.Department of Pathology Sciences, Universidade Estadual de Londrina (UEL), Rodovia Celso Garcia Cid|Pr 445 Km 380, Londrina, PR CEP 86.057-970, Brazil.Department of Pathology Sciences, Universidade Estadual de Londrina (UEL), Rodovia Celso Garcia Cid|Pr 445 Km 380, Londrina, PR CEP 86.057-970, Brazil.Department of Pathology Sciences, Universidade Estadual de Londrina (UEL), Rodovia Celso Garcia Cid|Pr 445 Km 380, Londrina, PR CEP 86.057-970, Brazil.Department of Pathology Sciences, Universidade Estadual de Londrina (UEL), Rodovia Celso Garcia Cid|Pr 445 Km 380, Londrina, PR CEP 86.057-970, Brazil.Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, n 5790 Bloco O-33, Maringá, PR CEP 87020-900, Brazil. Electronic address: jnzanoni@uem.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32474374

Citation

Martins-Perles, Juliana Vanessa Colombo, et al. "Quercetin Increases Bioavailability of Nitric Oxide in the Jejunum of Euglycemic and Diabetic Rats and Induces Neuronal Plasticity in the Myenteric Plexus." Autonomic Neuroscience : Basic & Clinical, vol. 227, 2020, p. 102675.
Martins-Perles JVC, Bossolani GDP, Zignani I, et al. Quercetin increases bioavailability of nitric oxide in the jejunum of euglycemic and diabetic rats and induces neuronal plasticity in the myenteric plexus. Auton Neurosci. 2020;227:102675.
Martins-Perles, J. V. C., Bossolani, G. D. P., Zignani, I., de Souza, S. R. G., Frez, F. C. V., de Souza Melo, C. G., Barili, E., de Souza Neto, F. P., Guarnier, F. A., Armani, A. L. C., Cecchini, R., & Zanoni, J. N. (2020). Quercetin increases bioavailability of nitric oxide in the jejunum of euglycemic and diabetic rats and induces neuronal plasticity in the myenteric plexus. Autonomic Neuroscience : Basic & Clinical, 227, 102675. https://doi.org/10.1016/j.autneu.2020.102675
Martins-Perles JVC, et al. Quercetin Increases Bioavailability of Nitric Oxide in the Jejunum of Euglycemic and Diabetic Rats and Induces Neuronal Plasticity in the Myenteric Plexus. Auton Neurosci. 2020;227:102675. PubMed PMID: 32474374.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quercetin increases bioavailability of nitric oxide in the jejunum of euglycemic and diabetic rats and induces neuronal plasticity in the myenteric plexus. AU - Martins-Perles,Juliana Vanessa Colombo, AU - Bossolani,Gleison Daion Piovezana, AU - Zignani,Isabela, AU - de Souza,Sara Raquel Garcia, AU - Frez,Flávia Cristina Vieira, AU - de Souza Melo,Carina Guimarães, AU - Barili,Emerson, AU - de Souza Neto,Fernando Pinheiro, AU - Guarnier,Flávia Alessandra, AU - Armani,Alessandra Lourenço Cecchini, AU - Cecchini,Rubens, AU - Zanoni,Jacqueline Nelisis, Y1 - 2020/05/06/ PY - 2019/11/19/received PY - 2020/03/06/revised PY - 2020/04/24/accepted PY - 2020/6/1/pubmed PY - 2020/6/1/medline PY - 2020/6/1/entrez KW - Diabetes mellitus KW - Myenteric varicosities KW - Neuroplasticity KW - VIP SP - 102675 EP - 102675 JF - Autonomic neuroscience : basic & clinical JO - Auton Neurosci VL - 227 N2 - Considering the antioxidant, neuroprotective, inflammatory and nitric oxide modulatory actions of quercetin, the aim of this study was to test the effect of quercetin administration in drinking water (40 mg/day/rat) on neuronal nitric oxide synthase (nNOS), vasoactive intestinal peptide (VIP), overall population of myenteric neurons (HuC/D) and nitric oxide (NO) levels in the jejunal samples from diabetic rats. Male Wistar rats were distributed into four groups (8 rats per group): euglycemic (E), euglycemic administered with quercetin (E+Q), diabetic (D) and diabetic administered with quercetin (D+Q). Rats were induced to diabetes with streptozotocin (35mg/kg/iv) and, after 120 days, the proximal jejunum were collected and processed for immunohistochemical (VIP, nNOS and HuC/D) and chemiluminescence (quantification of tissue NO levels) techniques. Diabetes mellitus reduced the number of nNOS-IR (immunoreactive) (p <0.05) and HuC/D-IR (p <0.001) neurons, however, promoted an increased morphometric area of nNOS-IR neurons (p <0.001) and VIP-IR varicosities (p <0.05). In D+Q group, neuroplasticity effects were observed on HuC/D-IR neurons, accompanied by a reduction of cell body area of neurons nNOS- and VIP-IR varicosities (p <0.05). The NO levels were increased in the E+Q (p <0.05) and D+Q group (p <0.001) compared to the control group. In conclusion, the results showed that quercetin supplementation increased the bioavailability of NO in the jejunum in euglycemic and mitigate the effects of diabetes on nNOS-IR neurons and VIP-IR varicosities in the myenteric plexus of diabetic rats. SN - 1872-7484 UR - https://www.unboundmedicine.com/medline/citation/32474374/Quercetin_increases_bioavailability_of_nitric_oxide_in_the_jejunum_of_euglycemic_and_diabetic_rats_and_induces_neuronal_plasticity_in_the_myenteric_plexus L2 - https://linkinghub.elsevier.com/retrieve/pii/S1566-0702(20)30109-0 DB - PRIME DP - Unbound Medicine ER -