Tags

Type your tag names separated by a space and hit enter

Effects of Oxidation of Human Serum Albumin on the Binding of Aripiprazole.
Biol Pharm Bull. 2020; 43(6):1023-1026.BP

Abstract

Aripiprazole (ARP) is one of antipsychotics and binds to human serum albumin (HSA) with a high affinity. In this study, we investigated the binding characteristics of ARP to oxidized HSA as observed in chronic disease conditions. Oxidized HSAs were prepared using chloramine-T (CT-HSA) or metal-catalyzed oxidation system (MCO-HSA) in vitro, respectively. An increase in the carbonyl content was confirmed in oxidized HSAs. From the results of circular dichroism (CD) and tryptophan fluorescence spectra, no significant structural change of oxidized HSAs was observed. These results indicate that prepared HSAs are mildly oxidized and well reflects the status of HSA during chronic diseases. However, oxidized HSAs were observed to have a significant decrease in binding to ARP. The results of the induced CD spectrum suggested that ARP bound to oxidized HSAs with a similar orientation. These results suggest that oxidation of HSA during chronic disease state significantly affected the microenvironment of the binding site for ARP and binding capacity of HSA to ARP.

Authors+Show Affiliations

Faculty of Pharmaceutical Sciences, Sojo Univerity.Faculty of Pharmaceutical Sciences, Sojo Univerity.School of Pharmacy, Manash University Malaysia.Faculty of Pharmaceutical Sciences, Sojo Univerity.Faculty of Pharmaceutical Sciences, Sojo Univerity. DDS Research Institute, Sojo University.Faculty of Pharmaceutical Sciences, Sojo Univerity. DDS Research Institute, Sojo University.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32475912

Citation

Sakurama, Keiki, et al. "Effects of Oxidation of Human Serum Albumin On the Binding of Aripiprazole." Biological & Pharmaceutical Bulletin, vol. 43, no. 6, 2020, pp. 1023-1026.
Sakurama K, Nishi K, Chuang VTG, et al. Effects of Oxidation of Human Serum Albumin on the Binding of Aripiprazole. Biol Pharm Bull. 2020;43(6):1023-1026.
Sakurama, K., Nishi, K., Chuang, V. T. G., Hashimoto, M., Yamasaki, K., & Otagiri, M. (2020). Effects of Oxidation of Human Serum Albumin on the Binding of Aripiprazole. Biological & Pharmaceutical Bulletin, 43(6), 1023-1026. https://doi.org/10.1248/bpb.b20-00205
Sakurama K, et al. Effects of Oxidation of Human Serum Albumin On the Binding of Aripiprazole. Biol Pharm Bull. 2020;43(6):1023-1026. PubMed PMID: 32475912.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Oxidation of Human Serum Albumin on the Binding of Aripiprazole. AU - Sakurama,Keiki, AU - Nishi,Koji, AU - Chuang,Victor Tuan Giam, AU - Hashimoto,Mai, AU - Yamasaki,Keishi, AU - Otagiri,Masaki, PY - 2020/6/2/entrez KW - albumin KW - aripiprazole KW - oxidation KW - protein binding SP - 1023 EP - 1026 JF - Biological & pharmaceutical bulletin JO - Biol. Pharm. Bull. VL - 43 IS - 6 N2 - Aripiprazole (ARP) is one of antipsychotics and binds to human serum albumin (HSA) with a high affinity. In this study, we investigated the binding characteristics of ARP to oxidized HSA as observed in chronic disease conditions. Oxidized HSAs were prepared using chloramine-T (CT-HSA) or metal-catalyzed oxidation system (MCO-HSA) in vitro, respectively. An increase in the carbonyl content was confirmed in oxidized HSAs. From the results of circular dichroism (CD) and tryptophan fluorescence spectra, no significant structural change of oxidized HSAs was observed. These results indicate that prepared HSAs are mildly oxidized and well reflects the status of HSA during chronic diseases. However, oxidized HSAs were observed to have a significant decrease in binding to ARP. The results of the induced CD spectrum suggested that ARP bound to oxidized HSAs with a similar orientation. These results suggest that oxidation of HSA during chronic disease state significantly affected the microenvironment of the binding site for ARP and binding capacity of HSA to ARP. SN - 1347-5215 UR - https://www.unboundmedicine.com/medline/citation/32475912/Effects_of_Oxidation_of_Human_Serum_Albumin_on_the_Binding_of_Aripiprazole L2 - https://dx.doi.org/10.1248/bpb.b20-00205 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.