Tags

Type your tag names separated by a space and hit enter

Development and validation of liquid chromatography-tandem mass spectrometry targeted screening of 16 fentanyl analogs and U-47700 in hair: Application to 137 authentic samples.
Drug Test Anal. 2020 Sep; 12(9):1298-1308.DT

Abstract

This study was to validate a LC-MS/MS method for the determination of 17 new synthetic opioids (NSOs) in hair including 3-fluorofentanyl, 3-methylfentanyl, acetylfentanyl, acetylnorfentanyl, alfentanyl, butyrylfentanyl, butyrylnorfentanyl, carfentanil, fentanyl, furanylfentanyl, furanylnorfentanyl, methoxyacetylfentanyl, norcarfentanil, norfentanyl, ocfentanil, sufentanil, and U-47700, and to apply it to 137 authentic samples. Twenty milligrams of hair was decontaminated in dichloromethane and underwent liquid extraction. 10 μL of the reconstituted residue were injected onto the system. The separation was performed in 12 minutes in a gradient mode at a flow rate of 300 μL/min using a Hypersyl Gold PFP column (100 × 2.1 mm i.d., 1.9 μm) maintained at 30°C. Compounds were detected in positive ionization and MRM modes using a TSQ Endura mass spectrometer (ThermoFisher). The method was validated according to EMA guidelines. The LLOQ was in the range 1-50 pg/mg, and the calibration ranged from the LLOQ-1000 pg/mg. Intra- and inter-day accuracy (bias) and precision were < 15%. Extraction recoveries of parent drugs and metabolites were 74-120% and 7-62%, respectively. The matrix effect was in the range 59-126% (CVs ≤ 12.9%). Fentanyl was found in six cases at concentrations of < 1-1650 pg/mg (n = 14 segments). Five fentanyl analogs were quantified in two cases: 3-fluorofentanyl (25-150 pg/mg, n = 5), furanylfentanyl (15-500 pg/mg, n = 5), methoxyacetylfentanyl (500-600 pg/mg, n = 2), acetylfentanyl (1 pg/mg, n = 2), carfentanyl (2.5-3 pg/mg, n = 2). This fully validated method allowed us to test for the first time 3-fluorofentanyl and norcarfentanil in hair among 15 other NSOs, and brings new data regarding 3-fluorofentanyl and methoxyacetylfentanyl hair concentrations.

Authors+Show Affiliations

Department of Pharmacology and Toxicology, Paris-Saclay University (Versailles Saint-Quentin-en-Yvelines University), Inserm U-1173, Raymond Poincaré Hospital, AP-HP, Garches, France.Department of Pharmacology and Toxicology, Paris-Saclay University (Versailles Saint-Quentin-en-Yvelines University), Inserm U-1173, Raymond Poincaré Hospital, AP-HP, Garches, France.Department of Pharmacology and Toxicology, Paris-Saclay University (Versailles Saint-Quentin-en-Yvelines University), Inserm U-1173, Raymond Poincaré Hospital, AP-HP, Garches, France.Addiction clinic, Pitié Salpétrière hospital, AP-HP, Paris, France.Addiction clinic, Pitié Salpétrière hospital, AP-HP, Paris, France.Department of Pharmacology and Toxicology, Paris-Saclay University (Versailles Saint-Quentin-en-Yvelines University), Inserm U-1173, Raymond Poincaré Hospital, AP-HP, Garches, France.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32476263

Citation

Larabi, Islam Amine, et al. "Development and Validation of Liquid Chromatography-tandem Mass Spectrometry Targeted Screening of 16 Fentanyl Analogs and U-47700 in Hair: Application to 137 Authentic Samples." Drug Testing and Analysis, vol. 12, no. 9, 2020, pp. 1298-1308.
Larabi IA, Martin M, Etting I, et al. Development and validation of liquid chromatography-tandem mass spectrometry targeted screening of 16 fentanyl analogs and U-47700 in hair: Application to 137 authentic samples. Drug Test Anal. 2020;12(9):1298-1308.
Larabi, I. A., Martin, M., Etting, I., Pfau, G., Edel, Y., & Alvarez, J. C. (2020). Development and validation of liquid chromatography-tandem mass spectrometry targeted screening of 16 fentanyl analogs and U-47700 in hair: Application to 137 authentic samples. Drug Testing and Analysis, 12(9), 1298-1308. https://doi.org/10.1002/dta.2868
Larabi IA, et al. Development and Validation of Liquid Chromatography-tandem Mass Spectrometry Targeted Screening of 16 Fentanyl Analogs and U-47700 in Hair: Application to 137 Authentic Samples. Drug Test Anal. 2020;12(9):1298-1308. PubMed PMID: 32476263.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of liquid chromatography-tandem mass spectrometry targeted screening of 16 fentanyl analogs and U-47700 in hair: Application to 137 authentic samples. AU - Larabi,Islam Amine, AU - Martin,Marie, AU - Etting,Isabelle, AU - Pfau,Gregory, AU - Edel,Yves, AU - Alvarez,Jean Claude, Y1 - 2020/07/07/ PY - 2020/05/05/received PY - 2020/05/27/revised PY - 2020/05/29/accepted PY - 2020/6/2/pubmed PY - 2020/6/2/medline PY - 2020/6/2/entrez KW - 3-fluorofentanyl KW - carfentanil KW - hair analysis KW - methoxyacetylfentanyl KW - opioids SP - 1298 EP - 1308 JF - Drug testing and analysis JO - Drug Test Anal VL - 12 IS - 9 N2 - This study was to validate a LC-MS/MS method for the determination of 17 new synthetic opioids (NSOs) in hair including 3-fluorofentanyl, 3-methylfentanyl, acetylfentanyl, acetylnorfentanyl, alfentanyl, butyrylfentanyl, butyrylnorfentanyl, carfentanil, fentanyl, furanylfentanyl, furanylnorfentanyl, methoxyacetylfentanyl, norcarfentanil, norfentanyl, ocfentanil, sufentanil, and U-47700, and to apply it to 137 authentic samples. Twenty milligrams of hair was decontaminated in dichloromethane and underwent liquid extraction. 10 μL of the reconstituted residue were injected onto the system. The separation was performed in 12 minutes in a gradient mode at a flow rate of 300 μL/min using a Hypersyl Gold PFP column (100 × 2.1 mm i.d., 1.9 μm) maintained at 30°C. Compounds were detected in positive ionization and MRM modes using a TSQ Endura mass spectrometer (ThermoFisher). The method was validated according to EMA guidelines. The LLOQ was in the range 1-50 pg/mg, and the calibration ranged from the LLOQ-1000 pg/mg. Intra- and inter-day accuracy (bias) and precision were < 15%. Extraction recoveries of parent drugs and metabolites were 74-120% and 7-62%, respectively. The matrix effect was in the range 59-126% (CVs ≤ 12.9%). Fentanyl was found in six cases at concentrations of < 1-1650 pg/mg (n = 14 segments). Five fentanyl analogs were quantified in two cases: 3-fluorofentanyl (25-150 pg/mg, n = 5), furanylfentanyl (15-500 pg/mg, n = 5), methoxyacetylfentanyl (500-600 pg/mg, n = 2), acetylfentanyl (1 pg/mg, n = 2), carfentanyl (2.5-3 pg/mg, n = 2). This fully validated method allowed us to test for the first time 3-fluorofentanyl and norcarfentanil in hair among 15 other NSOs, and brings new data regarding 3-fluorofentanyl and methoxyacetylfentanyl hair concentrations. SN - 1942-7611 UR - https://www.unboundmedicine.com/medline/citation/32476263/Development_and_validation_of_liquid_chromatography_tandem_mass_spectrometry_targeted_screening_of_16_fentanyl_analogs_and_U_47700_in_hair:_Application_to_137_authentic_samples_ L2 - https://doi.org/10.1002/dta.2868 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.