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Protecting HaCaT cells from ionizing radiation using persimmon tannin-Aloe gel composite.
Pharm Biol. 2020 Dec; 58(1):510-517.PB

Abstract

Context:

Persimmon tannin (extract of Diospyros kaki L.f [Ebenaceae]) and Aloe gel (extract of Aloe vera (L.) Burm.f. [Asphodelaceae]) are known as anti-radiation agents. However, radiation resistance of the persimmon tannin-Aloe gel composite remains inconclusive.

Objective:

To investigate the capacity of the persimmon tannin-Aloe gel composite to protect against ionising radiation at the cellular level.Materials and methods: HaCaT (human epidermal keratinocytes) cells were pre-treated with PT-A-1 (the mass ratio of persimmon tannin and Aloe gel was 2:1) or the single component (persimmon tannin or Aloe gel) at various concentrations (0, 50, 100, 200, 400, 800 μg/mL. Control group: medium with no HaCaT cells), and then radiated with X-rays (radiation dose: 4, 8, 12, 16, and 20 Gy). Cell viability, cell apoptosis, and radiation-induced intracellular reactive oxygen species (ROS) generation were analysed by CCK-8, Hoechst 33258 staining/flow cytometry, and 2',7'-dichlorfluorescein diacetate (DCFH-DA) assay, respectively, for 12 or 24 h incubation after radiation.

Results:

The optimal radiation dose and post-radiation incubation period were determined to be 8 Gy and 12 h. CCK-8 activity detection showed that the cell activity was 77.85% (p < 0.05, IC50 = 55.67 μg/mL). The apoptotic rate was the lowest (4.32%) at 200 μg/mL of PT-A-1 towards HaCaT cells. ROS production was the most effectively suppressed by 200 μg/mL PT-A-1 towards HaCaT cells.Discussion and conclusions: The persimmon tannin-Aloe gel composite has good radioprotective effect, and which will facilitate its clinic application as a potential natural anti-radiation agent in future.

Authors+Show Affiliations

School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin, China.School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin, China.School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin, China.China Nonferrous Metal (Guilin) Geology for Mineral Co., Ltd, Guilin, China.School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin, China.China Nonferrous Metal (Guilin) Geology for Mineral Co., Ltd, Guilin, China.China Nonferrous Metal (Guilin) Geology for Mineral Co., Ltd, Guilin, China.School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, China.School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32476533

Citation

Qian, Xi, et al. "Protecting HaCaT Cells From Ionizing Radiation Using Persimmon tannin-Aloe Gel Composite." Pharmaceutical Biology, vol. 58, no. 1, 2020, pp. 510-517.
Qian X, Wang Z, Ning J, et al. Protecting HaCaT cells from ionizing radiation using persimmon tannin-Aloe gel composite. Pharm Biol. 2020;58(1):510-517.
Qian, X., Wang, Z., Ning, J., Qin, C., Gao, L., He, N., Lin, D., Zhou, Z., & Li, G. (2020). Protecting HaCaT cells from ionizing radiation using persimmon tannin-Aloe gel composite. Pharmaceutical Biology, 58(1), 510-517. https://doi.org/10.1080/13880209.2020.1767158
Qian X, et al. Protecting HaCaT Cells From Ionizing Radiation Using Persimmon tannin-Aloe Gel Composite. Pharm Biol. 2020;58(1):510-517. PubMed PMID: 32476533.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protecting HaCaT cells from ionizing radiation using persimmon tannin-Aloe gel composite. AU - Qian,Xi, AU - Wang,Zhongmin, AU - Ning,Jinliang, AU - Qin,Chaoke, AU - Gao,Lin, AU - He,Na, AU - Lin,Dahong, AU - Zhou,Zhide, AU - Li,Guiyin, PY - 2020/6/2/entrez KW - Human epidermal keratinocytes cells KW - Radiation resistance KW - X-rays SP - 510 EP - 517 JF - Pharmaceutical biology JO - Pharm Biol VL - 58 IS - 1 N2 - Context: Persimmon tannin (extract of Diospyros kaki L.f [Ebenaceae]) and Aloe gel (extract of Aloe vera (L.) Burm.f. [Asphodelaceae]) are known as anti-radiation agents. However, radiation resistance of the persimmon tannin-Aloe gel composite remains inconclusive.Objective: To investigate the capacity of the persimmon tannin-Aloe gel composite to protect against ionising radiation at the cellular level.Materials and methods: HaCaT (human epidermal keratinocytes) cells were pre-treated with PT-A-1 (the mass ratio of persimmon tannin and Aloe gel was 2:1) or the single component (persimmon tannin or Aloe gel) at various concentrations (0, 50, 100, 200, 400, 800 μg/mL. Control group: medium with no HaCaT cells), and then radiated with X-rays (radiation dose: 4, 8, 12, 16, and 20 Gy). Cell viability, cell apoptosis, and radiation-induced intracellular reactive oxygen species (ROS) generation were analysed by CCK-8, Hoechst 33258 staining/flow cytometry, and 2',7'-dichlorfluorescein diacetate (DCFH-DA) assay, respectively, for 12 or 24 h incubation after radiation.Results: The optimal radiation dose and post-radiation incubation period were determined to be 8 Gy and 12 h. CCK-8 activity detection showed that the cell activity was 77.85% (p < 0.05, IC50 = 55.67 μg/mL). The apoptotic rate was the lowest (4.32%) at 200 μg/mL of PT-A-1 towards HaCaT cells. ROS production was the most effectively suppressed by 200 μg/mL PT-A-1 towards HaCaT cells.Discussion and conclusions: The persimmon tannin-Aloe gel composite has good radioprotective effect, and which will facilitate its clinic application as a potential natural anti-radiation agent in future. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/32476533/Protecting_HaCaT_cells_from_ionizing_radiation_using_persimmon_tannin-Aloe_gel_composite L2 - http://www.tandfonline.com/doi/full/10.1080/13880209.2020.1767158 DB - PRIME DP - Unbound Medicine ER -
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