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Tissue-resident macrophages promote extracellular matrix homeostasis in the mammary gland stroma of nulliparous mice.
Elife. 2020 Jun 01; 9E

Abstract

Tissue-resident macrophages in the mammary gland are found in close association with epithelial structures and within the adipose stroma, and are important for mammary gland development and tissue homeostasis. Macrophages have been linked to ductal development in the virgin mammary gland, but less is known regarding the effects of macrophages on the adipose stroma. Using transcriptional profiling and single-cell RNA sequencing approaches, we identify a distinct resident stromal macrophage subpopulation within the mouse nulliparous mammary gland that is characterized by the expression of Lyve-1, a receptor for the extracellular matrix (ECM) component hyaluronan. This subpopulation is enriched in genes associated with ECM remodeling and is specifically associated with hyaluronan-rich regions within the adipose stroma and fibrous capsule of the virgin mammary gland. Furthermore, macrophage depletion leads to enhanced accumulation of hyaluronan-associated ECM in the adipose-associated stroma, indicating that resident macrophages are important for maintaining homeostasis within the nulliparous mammary gland stroma.

Authors+Show Affiliations

Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, United States.Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, United States.University of Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, United States.Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, United States.Comparative and Molecular Biosciences Graduate Program, University of Minnesota, Minneapolis, United States.Department of Biochemistry and Molecular Biology, Tulane Cancer Center, Tulane School of Medicine, New Orleans, United States. Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, United States. Masonic Cancer Center, University of Minnesota, Minneapolis, United States.Department of Biochemistry and Molecular Biology, Tulane Cancer Center, Tulane School of Medicine, New Orleans, United States.Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, United States. Masonic Cancer Center, University of Minnesota, Minneapolis, United States. Center for Immunology, University of Minnesota, Minneapolis, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32479261

Citation

Wang, Ying, et al. "Tissue-resident Macrophages Promote Extracellular Matrix Homeostasis in the Mammary Gland Stroma of Nulliparous Mice." ELife, vol. 9, 2020.
Wang Y, Chaffee TS, LaRue RS, et al. Tissue-resident macrophages promote extracellular matrix homeostasis in the mammary gland stroma of nulliparous mice. Elife. 2020;9.
Wang, Y., Chaffee, T. S., LaRue, R. S., Huggins, D. N., Witschen, P. M., Ibrahim, A. M., Nelson, A. C., Machado, H. L., & Schwertfeger, K. L. (2020). Tissue-resident macrophages promote extracellular matrix homeostasis in the mammary gland stroma of nulliparous mice. ELife, 9. https://doi.org/10.7554/eLife.57438
Wang Y, et al. Tissue-resident Macrophages Promote Extracellular Matrix Homeostasis in the Mammary Gland Stroma of Nulliparous Mice. Elife. 2020 Jun 1;9 PubMed PMID: 32479261.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue-resident macrophages promote extracellular matrix homeostasis in the mammary gland stroma of nulliparous mice. AU - Wang,Ying, AU - Chaffee,Thomas S, AU - LaRue,Rebecca S, AU - Huggins,Danielle N, AU - Witschen,Patrice M, AU - Ibrahim,Ayman M, AU - Nelson,Andrew C, AU - Machado,Heather L, AU - Schwertfeger,Kathryn L, Y1 - 2020/06/01/ PY - 2020/03/31/received PY - 2020/05/31/accepted PY - 2020/6/2/pubmed PY - 2020/6/2/medline PY - 2020/6/2/entrez KW - developmental biology KW - extracellular matrix KW - immunology KW - inflammation KW - macrophage KW - mammary gland KW - mouse KW - stroma JF - eLife JO - Elife VL - 9 N2 - Tissue-resident macrophages in the mammary gland are found in close association with epithelial structures and within the adipose stroma, and are important for mammary gland development and tissue homeostasis. Macrophages have been linked to ductal development in the virgin mammary gland, but less is known regarding the effects of macrophages on the adipose stroma. Using transcriptional profiling and single-cell RNA sequencing approaches, we identify a distinct resident stromal macrophage subpopulation within the mouse nulliparous mammary gland that is characterized by the expression of Lyve-1, a receptor for the extracellular matrix (ECM) component hyaluronan. This subpopulation is enriched in genes associated with ECM remodeling and is specifically associated with hyaluronan-rich regions within the adipose stroma and fibrous capsule of the virgin mammary gland. Furthermore, macrophage depletion leads to enhanced accumulation of hyaluronan-associated ECM in the adipose-associated stroma, indicating that resident macrophages are important for maintaining homeostasis within the nulliparous mammary gland stroma. SN - 2050-084X UR - https://www.unboundmedicine.com/medline/citation/32479261/Tissue-resident_macrophages_promote_extracellular_matrix_homeostasis_in_the_mammary_gland_stroma_of_nulliparous_mice L2 - https://doi.org/10.7554/eLife.57438 DB - PRIME DP - Unbound Medicine ER -
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