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Simultaneous quantification of ergot and tropane alkaloids in bread in the Netherlands by LC-MS/MS.
Food Addit Contam Part B Surveill. 2020 Jun 01 [Online ahead of print]FA

Abstract

Atropine and scopolamine are tropane alkaloids (TAs), which are regulated for cereal-based foods for children in the EU. For ergot alkaloids (EAs) in cereals and cereal-based food harmonised legislation is not yet established. A fast and straightforward method, which employs extraction by acidified water/methanol followed by ultra-filtration prior to analysis by LC-MS/MS, was validated in bread for 20 EAs and six TAs. LOQs for individual alkaloids ranged from 0.3 to 1.2 µg kg-1, while recoveries ranged from 65% to 94% and repeatability from 3.4% to 17%. A survey was conducted in the Netherlands on 40 retail samples of bread (wheat, rye, wheat-rye, multi-grain) collected in 2014 and 2018. TAs, including atropine and scopolamine, were not detected. Eighteen different EAs were detected and total levels varied between <LOQ and 335 µg kg-1. Since EAs were detected in a wide concentration range, it is recommended to monitor their occurrence in bread more regularly.

Authors+Show Affiliations

Wageningen Food Safety Research, Part of Wageningen University & Research , Wageningen, the Netherlands.Wageningen Food Safety Research, Part of Wageningen University & Research , Wageningen, the Netherlands.Wageningen Food Safety Research, Part of Wageningen University & Research , Wageningen, the Netherlands.Netherlands Food and Consumer Product Safety Authority (NVWA) , Utrecht, Directorate Enforcement, Department Expertise, the Netherlands.Wageningen Food Safety Research, Part of Wageningen University & Research , Wageningen, the Netherlands.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32482157

Citation

Veršilovskis, Aleksandrs, et al. "Simultaneous Quantification of Ergot and Tropane Alkaloids in Bread in the Netherlands By LC-MS/MS." Food Additives & Contaminants. Part B, Surveillance, 2020, pp. 1-9.
Veršilovskis A, Mulder PPJ, Pereboom-de Fauw DPKH, et al. Simultaneous quantification of ergot and tropane alkaloids in bread in the Netherlands by LC-MS/MS. Food Addit Contam Part B Surveill. 2020.
Veršilovskis, A., Mulder, P. P. J., Pereboom-de Fauw, D. P. K. H., de Stoppelaar, J., & de Nijs, M. (2020). Simultaneous quantification of ergot and tropane alkaloids in bread in the Netherlands by LC-MS/MS. Food Additives & Contaminants. Part B, Surveillance, 1-9. https://doi.org/10.1080/19393210.2020.1771777
Veršilovskis A, et al. Simultaneous Quantification of Ergot and Tropane Alkaloids in Bread in the Netherlands By LC-MS/MS. Food Addit Contam Part B Surveill. 2020 Jun 1;1-9. PubMed PMID: 32482157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous quantification of ergot and tropane alkaloids in bread in the Netherlands by LC-MS/MS. AU - Veršilovskis,Aleksandrs, AU - Mulder,Patrick P J, AU - Pereboom-de Fauw,Diana P K H, AU - de Stoppelaar,Joyce, AU - de Nijs,Monique, Y1 - 2020/06/01/ PY - 2020/6/3/entrez KW - Ergot alkaloids KW - LC-MS/MS KW - bread KW - quantification KW - survey KW - tropane alkaloids SP - 1 EP - 9 JF - Food additives & contaminants. Part B, Surveillance JO - Food Addit Contam Part B Surveill N2 - Atropine and scopolamine are tropane alkaloids (TAs), which are regulated for cereal-based foods for children in the EU. For ergot alkaloids (EAs) in cereals and cereal-based food harmonised legislation is not yet established. A fast and straightforward method, which employs extraction by acidified water/methanol followed by ultra-filtration prior to analysis by LC-MS/MS, was validated in bread for 20 EAs and six TAs. LOQs for individual alkaloids ranged from 0.3 to 1.2 µg kg-1, while recoveries ranged from 65% to 94% and repeatability from 3.4% to 17%. A survey was conducted in the Netherlands on 40 retail samples of bread (wheat, rye, wheat-rye, multi-grain) collected in 2014 and 2018. TAs, including atropine and scopolamine, were not detected. Eighteen different EAs were detected and total levels varied between <LOQ and 335 µg kg-1. Since EAs were detected in a wide concentration range, it is recommended to monitor their occurrence in bread more regularly. SN - 1939-3229 UR - https://www.unboundmedicine.com/medline/citation/32482157/Simultaneous_quantification_of_ergot_and_tropane_alkaloids_in_bread_in_the_Netherlands_by_LC-MS/MS L2 - http://www.tandfonline.com/doi/full/10.1080/19393210.2020.1771777 DB - PRIME DP - Unbound Medicine ER -
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