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No evidence of severe acute respiratory syndrome-coronavirus 2 in semen of males recovering from coronavirus disease 2019.
Fertil Steril. 2020 06; 113(6):1135-1139.FS

Abstract

OBJECTIVE

To describe detection of severe acute respiratory syndrome (SARS)-coronavirus 2 (CoV-2) in seminal fluid of patients recovering from coronavirus disease 2019 (COVID-19) and to describe the expression profile of angiotensin-converting enzyme 2 (ACE2) and Transmembrane Serine Protease 2 (TMPRSS2) within the testicle.

DESIGN

Observational, cross-sectional study.

SETTING

Tertiary referral center.

PATIENT(S)

Thirty-four adult Chinese males diagnosed with COVID-19 through confirmatory quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) from pharyngeal swab samples.

INTERVENTION(S)

None.

MAIN OUTCOME MEASURE(S)

Identification of SARS-CoV-2 on qRT-PCR of single ejaculated semen samples. Semen quality was not assessed. Expression patterns of ACE2 and TMPRSS2 in the human testis are explored through previously published single-cell transcriptome datasets.

RESULT(S)

Six patients (19%) demonstrated scrotal discomfort suggestive of viral orchitis around the time of COVID-19 confirmation. Severe acute respiratory syndrome-CoV-2 was not detected in semen after a median of 31 days (interquartile range, 29-36 days) from COVID-19 diagnosis. Single-cell transcriptome analysis demonstrates sparse expression of ACE2 and TMPRSS2, with almost no overlapping gene expression.

CONCLUSION(S)

Severe acute respiratory syndrome-CoV-2 was not detected in the semen of patients recovering from COVID-19 1 month after COVID-19 diagnosis. Angiotensin-converting enzyme 2-mediated viral entry of SARS-CoV-2 into target host cells is unlikely to occur within the human testicle based on ACE2 and TMPRSS2 expression. The long-term effects of SARS-CoV-2 on male reproductive function remain unknown.

Links

PMC Free PDF

Authors+Show Affiliations

Pan F
Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Xiao X
Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Guo J
Division of Urology, Department of Surgery, University of Utah School of Medicine, Salt Lake City, Utah; Department of Oncological Sciences, Huntsman Cancer Institute, Salt Lake City, Utah.
Song Y
Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Li H
Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Patel DP
Division of Urology, Department of Surgery, University of Utah School of Medicine, Salt Lake City, Utah.
Spivak AM
Division of Infectious Disease, Department of Internal Medicine, University of Utah School of Medicine, Salt Lake City, Utah.
Alukal JP
Department of Urology, Columbia University Medical Center, New York, New York.
Zhang X
Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Xiong C
Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China; Wuhan Tongji Reproductive Medicine Hospital, Wuhan, People's Republic of China.
Li PS
Center for Male Reproductive Medicine and Microsurgery, Department of Urology, Weill Cornell Medicine of Cornell University, New York, New York.
Hotaling JM
Division of Urology, Department of Surgery, University of Utah School of Medicine, Salt Lake City, Utah. Electronic address: jim.hotaling@hsc.utah.edu.

MeSH

AdolescentAdultAngiotensin-Converting Enzyme 2BetacoronavirusCOVID-19COVID-19 TestingClinical Laboratory TechniquesCoronavirus InfectionsCross-Sectional StudiesHumansMaleMiddle AgedPandemicsPeptidyl-Dipeptidase APneumonia, ViralRNA-SeqReal-Time Polymerase Chain ReactionSARS-CoV-2SemenSerine EndopeptidasesTestisTime FactorsTranscriptomeVirus InternalizationYoung Adult

Pub Type(s)

Journal Article
Observational Study

Language

eng

PubMed ID

32482249

Clinical Trial Links

COVID-19 Detection Tests in Different Body Fluids
LOng COvid COmorbidities: Andrological, Reproductive, Sexual Dysfunctions in Patients Recovered From COVID-19

Citation

Pan, Feng, et al. "No Evidence of Severe Acute Respiratory Syndrome-coronavirus 2 in Semen of Males Recovering From Coronavirus Disease 2019." Fertility and Sterility, vol. 113, no. 6, 2020, pp. 1135-1139.
Pan F, Xiao X, Guo J, et al. No evidence of severe acute respiratory syndrome-coronavirus 2 in semen of males recovering from coronavirus disease 2019. Fertil Steril. 2020;113(6):1135-1139.
Pan, F., Xiao, X., Guo, J., Song, Y., Li, H., Patel, D. P., Spivak, A. M., Alukal, J. P., Zhang, X., Xiong, C., Li, P. S., & Hotaling, J. M. (2020). No evidence of severe acute respiratory syndrome-coronavirus 2 in semen of males recovering from coronavirus disease 2019. Fertility and Sterility, 113(6), 1135-1139. https://doi.org/10.1016/j.fertnstert.2020.04.024
Pan F, et al. No Evidence of Severe Acute Respiratory Syndrome-coronavirus 2 in Semen of Males Recovering From Coronavirus Disease 2019. Fertil Steril. 2020;113(6):1135-1139. PubMed PMID: 32482249.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - No evidence of severe acute respiratory syndrome-coronavirus 2 in semen of males recovering from coronavirus disease 2019. AU - Pan,Feng, AU - Xiao,Xingyuan, AU - Guo,Jingtao, AU - Song,Yarong, AU - Li,Honggang, AU - Patel,Darshan P, AU - Spivak,Adam M, AU - Alukal,Joseph P, AU - Zhang,Xiaoping, AU - Xiong,Chengliang, AU - Li,Philip S, AU - Hotaling,James M, Y1 - 2020/04/17/ PY - 2020/04/10/received PY - 2020/04/12/revised PY - 2020/04/13/accepted PY - 2020/6/3/entrez PY - 2020/6/3/pubmed PY - 2020/6/11/medline KW - COVID-19 KW - angiotensin-converting enzyme 2 KW - coronavirus KW - infertility KW - male KW - semen SP - 1135 EP - 1139 JF - Fertility and sterility JO - Fertil Steril VL - 113 IS - 6 N2 - OBJECTIVE: To describe detection of severe acute respiratory syndrome (SARS)-coronavirus 2 (CoV-2) in seminal fluid of patients recovering from coronavirus disease 2019 (COVID-19) and to describe the expression profile of angiotensin-converting enzyme 2 (ACE2) and Transmembrane Serine Protease 2 (TMPRSS2) within the testicle. DESIGN: Observational, cross-sectional study. SETTING: Tertiary referral center. PATIENT(S): Thirty-four adult Chinese males diagnosed with COVID-19 through confirmatory quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) from pharyngeal swab samples. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Identification of SARS-CoV-2 on qRT-PCR of single ejaculated semen samples. Semen quality was not assessed. Expression patterns of ACE2 and TMPRSS2 in the human testis are explored through previously published single-cell transcriptome datasets. RESULT(S): Six patients (19%) demonstrated scrotal discomfort suggestive of viral orchitis around the time of COVID-19 confirmation. Severe acute respiratory syndrome-CoV-2 was not detected in semen after a median of 31 days (interquartile range, 29-36 days) from COVID-19 diagnosis. Single-cell transcriptome analysis demonstrates sparse expression of ACE2 and TMPRSS2, with almost no overlapping gene expression. CONCLUSION(S): Severe acute respiratory syndrome-CoV-2 was not detected in the semen of patients recovering from COVID-19 1 month after COVID-19 diagnosis. Angiotensin-converting enzyme 2-mediated viral entry of SARS-CoV-2 into target host cells is unlikely to occur within the human testicle based on ACE2 and TMPRSS2 expression. The long-term effects of SARS-CoV-2 on male reproductive function remain unknown. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/32482249/No_evidence_of_severe_acute_respiratory_syndrome_coronavirus_2_in_semen_of_males_recovering_from_coronavirus_disease_2019_ DB - PRIME DP - Unbound Medicine ER -