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Transcriptome analysis of putative detoxification genes in the Asian citrus psyllid, Diaphorina citri.
Pest Manag Sci. 2020 Nov; 76(11):3857-3870.PM

Abstract

BACKGROUND

The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is a notorious pest that transmits the causal agent of huanglongbing (also called citrus greening disease). Resistance to insecticide in this destructive pest poses a serious threat to the citrus industry. To date, no systemic studies on genes coding for detoxification enzymes has been carried out on D. citri.

RESULTS

Multiple transcriptomes were generated through deep sequencing of RNA libraries. Candidate genes potentially involved in detoxification including cytochrome P450 monooxygenases (CYPs), glutathione S-transferases (GSTs), and esterases (ESTs) were systematically identified by searching the transcriptomes and a draft genome assembly. A total of 49, 14 and 20 genes were found encoding CYPs, GSTs, and ESTs, respectively, in D. citri. The total numbers of candidate detoxification genes were much smaller than the counterparts reported in other insect species, which may reflect the strict oligophagy of this insect species. Developmental stage- and tissue-specific expression patterns of the identified genes as well as their responses to insecticide treatments identified a small set of genes that could participate in detoxifying plant secondary metabolites and insecticides.

CONCLUSION

Our studies represent the most comprehensive investigation to date on identification, characterization and expression profiling of detoxification genes in D. citri. The information revealed in this study shall be useful in designing strategies to manage this important insect pest. © 2020 Society of Chemical Industry.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wu Z
Guangzhou City Key Laboratory of Subtropical Fruit Tree Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Pu X
Guangzhou City Key Laboratory of Subtropical Fruit Tree Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Shu B
Guangzhou City Key Laboratory of Subtropical Fruit Tree Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Bin S
Guangzhou City Key Laboratory of Subtropical Fruit Tree Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Lin J
Guangzhou City Key Laboratory of Subtropical Fruit Tree Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou, China.

MeSH

AnimalsCitrusGene Expression ProfilingHemipteraInsecticidesTranscriptome

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32483911

Citation

Wu, Zhongzhen, et al. "Transcriptome Analysis of Putative Detoxification Genes in the Asian Citrus Psyllid, Diaphorina Citri." Pest Management Science, vol. 76, no. 11, 2020, pp. 3857-3870.
Wu Z, Pu X, Shu B, et al. Transcriptome analysis of putative detoxification genes in the Asian citrus psyllid, Diaphorina citri. Pest Manag Sci. 2020;76(11):3857-3870.
Wu, Z., Pu, X., Shu, B., Bin, S., & Lin, J. (2020). Transcriptome analysis of putative detoxification genes in the Asian citrus psyllid, Diaphorina citri. Pest Management Science, 76(11), 3857-3870. https://doi.org/10.1002/ps.5937
Wu Z, et al. Transcriptome Analysis of Putative Detoxification Genes in the Asian Citrus Psyllid, Diaphorina Citri. Pest Manag Sci. 2020;76(11):3857-3870. PubMed PMID: 32483911.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptome analysis of putative detoxification genes in the Asian citrus psyllid, Diaphorina citri. AU - Wu,Zhongzhen, AU - Pu,Xinhua, AU - Shu,Benshui, AU - Bin,Shuying, AU - Lin,Jintian, Y1 - 2020/07/17/ PY - 2019/10/31/received PY - 2020/05/08/revised PY - 2020/06/01/accepted PY - 2020/6/3/pubmed PY - 2020/11/25/medline PY - 2020/6/3/entrez KW - Diaphorina citri KW - cytochrome P450 KW - detoxification KW - esterases KW - glutathione S-transferase SP - 3857 EP - 3870 JF - Pest management science JO - Pest Manag Sci VL - 76 IS - 11 N2 - BACKGROUND: The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is a notorious pest that transmits the causal agent of huanglongbing (also called citrus greening disease). Resistance to insecticide in this destructive pest poses a serious threat to the citrus industry. To date, no systemic studies on genes coding for detoxification enzymes has been carried out on D. citri. RESULTS: Multiple transcriptomes were generated through deep sequencing of RNA libraries. Candidate genes potentially involved in detoxification including cytochrome P450 monooxygenases (CYPs), glutathione S-transferases (GSTs), and esterases (ESTs) were systematically identified by searching the transcriptomes and a draft genome assembly. A total of 49, 14 and 20 genes were found encoding CYPs, GSTs, and ESTs, respectively, in D. citri. The total numbers of candidate detoxification genes were much smaller than the counterparts reported in other insect species, which may reflect the strict oligophagy of this insect species. Developmental stage- and tissue-specific expression patterns of the identified genes as well as their responses to insecticide treatments identified a small set of genes that could participate in detoxifying plant secondary metabolites and insecticides. CONCLUSION: Our studies represent the most comprehensive investigation to date on identification, characterization and expression profiling of detoxification genes in D. citri. The information revealed in this study shall be useful in designing strategies to manage this important insect pest. © 2020 Society of Chemical Industry. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/32483911/Transcriptome_analysis_of_putative_detoxification_genes_in_the_Asian_citrus_psyllid_Diaphorina_citri_ DB - PRIME DP - Unbound Medicine ER -