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Employing Nanostructured Scaffolds to Investigate the Mechanical Properties of Adult Mammalian Retinae Under Tension.
Int J Mol Sci. 2020 May 29; 21(11)IJ

Abstract

Numerous eye diseases are linked to biomechanical dysfunction of the retina. However, the underlying forces are almost impossible to quantify experimentally. Here, we show how biomechanical properties of adult neuronal tissues such as porcine retinae can be investigated under tension in a home-built tissue stretcher composed of nanostructured TiO2 scaffolds coupled to a self-designed force sensor. The employed TiO2 nanotube scaffolds allow for organotypic long-term preservation of adult tissues ex vivo and support strong tissue adhesion without the application of glues, a prerequisite for tissue investigations under tension. In combination with finite element calculations we found that the deformation behavior is highly dependent on the displacement rate which results in Young's moduli of (760-1270) Pa. Image analysis revealed that the elastic regime is characterized by a reversible shear deformation of retinal layers. For larger deformations, tissue destruction and sliding of retinal layers occurred with an equilibration between slip and stick at the interface of ruptured layers, resulting in a constant force during stretching. Since our study demonstrates how porcine eyes collected from slaughterhouses can be employed for ex vivo experiments, our study also offers new perspectives to investigate tissue biomechanics without excessive animal experiments.

Authors+Show Affiliations

Soft Matter Physics Division and Biotechnology & Biomedical Group, Peter-Debye-Institute for Soft Matter Physics, Leipzig University, Linnéstr. 5, 04103 Leipzig, Germany. Paul Flechsig Institute for Brain Research, Leipzig University, Liebigstr. 19, 04103 Leipzig, Germany.Soft Matter Physics Division and Biotechnology & Biomedical Group, Peter-Debye-Institute for Soft Matter Physics, Leipzig University, Linnéstr. 5, 04103 Leipzig, Germany.Division of Surface Physics, Department of Physics and Earth Sciences, Leipzig University and Leibniz Institute of Surface Engineering (IOM), Permoser Str. 15, 04318 Leipzig, Germany.Soft Matter Physics Division and Biotechnology & Biomedical Group, Peter-Debye-Institute for Soft Matter Physics, Leipzig University, Linnéstr. 5, 04103 Leipzig, Germany. Paul Flechsig Institute for Brain Research, Leipzig University, Liebigstr. 19, 04103 Leipzig, Germany. Present address: Department of Pediatrics, Weill Cornell Medical College, 1300 York Ave, New York 10065-4896, NY USA.Institute of Food Hygiene, Faculty of Veterinary Medicine, Augustusplatz 10, Leipzig University, 04109 Leipzig, Germany. Present address: Max Rubner-Institut (MRI), Federal Research Institute of Nutrition and Food, Department of Safety and Quality of Meat, E.-C.-Baumann-Str. 20, 95326 Kulmbach, Germany.Paul Flechsig Institute for Brain Research, Leipzig University, Liebigstr. 19, 04103 Leipzig, Germany.Division of Surface Physics, Department of Physics and Earth Sciences, Leipzig University and Leibniz Institute of Surface Engineering (IOM), Permoser Str. 15, 04318 Leipzig, Germany.Soft Matter Physics Division and Biotechnology & Biomedical Group, Peter-Debye-Institute for Soft Matter Physics, Leipzig University, Linnéstr. 5, 04103 Leipzig, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32485972

Citation

Juncheed, Kantida, et al. "Employing Nanostructured Scaffolds to Investigate the Mechanical Properties of Adult Mammalian Retinae Under Tension." International Journal of Molecular Sciences, vol. 21, no. 11, 2020.
Juncheed K, Kohlstrunk B, Friebe S, et al. Employing Nanostructured Scaffolds to Investigate the Mechanical Properties of Adult Mammalian Retinae Under Tension. Int J Mol Sci. 2020;21(11).
Juncheed, K., Kohlstrunk, B., Friebe, S., Dallacasagrande, V., Maurer, P., Reichenbach, A., Mayr, S. G., & Zink, M. (2020). Employing Nanostructured Scaffolds to Investigate the Mechanical Properties of Adult Mammalian Retinae Under Tension. International Journal of Molecular Sciences, 21(11). https://doi.org/10.3390/ijms21113889
Juncheed K, et al. Employing Nanostructured Scaffolds to Investigate the Mechanical Properties of Adult Mammalian Retinae Under Tension. Int J Mol Sci. 2020 May 29;21(11) PubMed PMID: 32485972.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Employing Nanostructured Scaffolds to Investigate the Mechanical Properties of Adult Mammalian Retinae Under Tension. AU - Juncheed,Kantida, AU - Kohlstrunk,Bernd, AU - Friebe,Sabrina, AU - Dallacasagrande,Valentina, AU - Maurer,Patric, AU - Reichenbach,Andreas, AU - Mayr,Stefan G, AU - Zink,Mareike, Y1 - 2020/05/29/ PY - 2020/05/11/received PY - 2020/05/25/revised PY - 2020/05/25/accepted PY - 2020/6/4/entrez PY - 2020/6/4/pubmed PY - 2020/6/4/medline KW - nanostructured scaffolds KW - nanotubes KW - porcine eyes KW - retina KW - tissue elasticity KW - tissue mechanics KW - tissue stretcher JF - International journal of molecular sciences JO - Int J Mol Sci VL - 21 IS - 11 N2 - Numerous eye diseases are linked to biomechanical dysfunction of the retina. However, the underlying forces are almost impossible to quantify experimentally. Here, we show how biomechanical properties of adult neuronal tissues such as porcine retinae can be investigated under tension in a home-built tissue stretcher composed of nanostructured TiO2 scaffolds coupled to a self-designed force sensor. The employed TiO2 nanotube scaffolds allow for organotypic long-term preservation of adult tissues ex vivo and support strong tissue adhesion without the application of glues, a prerequisite for tissue investigations under tension. In combination with finite element calculations we found that the deformation behavior is highly dependent on the displacement rate which results in Young's moduli of (760-1270) Pa. Image analysis revealed that the elastic regime is characterized by a reversible shear deformation of retinal layers. For larger deformations, tissue destruction and sliding of retinal layers occurred with an equilibration between slip and stick at the interface of ruptured layers, resulting in a constant force during stretching. Since our study demonstrates how porcine eyes collected from slaughterhouses can be employed for ex vivo experiments, our study also offers new perspectives to investigate tissue biomechanics without excessive animal experiments. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/32485972/Employing_Nanostructured_Scaffolds_to_Investigate_the_Mechanical_Properties_of_Adult_Mammalian_Retinae_Under_Tension L2 - https://www.mdpi.com/resolver?pii=ijms21113889 DB - PRIME DP - Unbound Medicine ER -
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