Tags

Type your tag names separated by a space and hit enter

Development of a Portable, Ultra-Rapid and Ultra-Sensitive Cell-Based Biosensor for the Direct Detection of the SARS-CoV-2 S1 Spike Protein Antigen.
Sensors (Basel). 2020 May 31; 20(11)S

Abstract

One of the key challenges of the recent COVID-19 pandemic is the ability to accurately estimate the number of infected individuals, particularly asymptomatic and/or early-stage patients. We herewith report the proof-of-concept development of a biosensor able to detect the SARS-CoV-2 S1 spike protein expressed on the surface of the virus. The biosensor is based on membrane-engineered mammalian cells bearing the human chimeric spike S1 antibody. We demonstrate that the attachment of the protein to the membrane-bound antibodies resulted in a selective and considerable change in the cellular bioelectric properties measured by means of a Bioelectric Recognition Assay. The novel biosensor provided results in an ultra-rapid manner (3 min), with a detection limit of 1 fg/mL and a semi-linear range of response between 10 fg and 1 μg/mL. In addition, no cross-reactivity was observed against the SARS-CoV-2 nucleocapsid protein. Furthermore, the biosensor was configured as a ready-to-use platform, including a portable read-out device operated via smartphone/tablet. In this way, we demonstrate that the novel biosensor can be potentially applied for the mass screening of SARS-CoV-2 surface antigens without prior sample processing, therefore offering a possible solution for the timely monitoring and eventual control of the global coronavirus pandemic.

Authors+Show Affiliations

Laboratory of Cell Technology, Department of Biotechnology, Agricultural University of Athens, EU-CONEXUS European University, 11855 Athens, Greece.Laboratory of Cell Technology, Department of Biotechnology, Agricultural University of Athens, EU-CONEXUS European University, 11855 Athens, Greece.Laboratory of Cell Technology, Department of Biotechnology, Agricultural University of Athens, EU-CONEXUS European University, 11855 Athens, Greece.Laboratory of Cell Technology, Department of Biotechnology, Agricultural University of Athens, EU-CONEXUS European University, 11855 Athens, Greece.

Pub Type(s)

Letter

Language

eng

PubMed ID

32486477

Citation

Mavrikou, Sophie, et al. "Development of a Portable, Ultra-Rapid and Ultra-Sensitive Cell-Based Biosensor for the Direct Detection of the SARS-CoV-2 S1 Spike Protein Antigen." Sensors (Basel, Switzerland), vol. 20, no. 11, 2020.
Mavrikou S, Moschopoulou G, Tsekouras V, et al. Development of a Portable, Ultra-Rapid and Ultra-Sensitive Cell-Based Biosensor for the Direct Detection of the SARS-CoV-2 S1 Spike Protein Antigen. Sensors (Basel). 2020;20(11).
Mavrikou, S., Moschopoulou, G., Tsekouras, V., & Kintzios, S. (2020). Development of a Portable, Ultra-Rapid and Ultra-Sensitive Cell-Based Biosensor for the Direct Detection of the SARS-CoV-2 S1 Spike Protein Antigen. Sensors (Basel, Switzerland), 20(11). https://doi.org/10.3390/s20113121
Mavrikou S, et al. Development of a Portable, Ultra-Rapid and Ultra-Sensitive Cell-Based Biosensor for the Direct Detection of the SARS-CoV-2 S1 Spike Protein Antigen. Sensors (Basel). 2020 May 31;20(11) PubMed PMID: 32486477.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a Portable, Ultra-Rapid and Ultra-Sensitive Cell-Based Biosensor for the Direct Detection of the SARS-CoV-2 S1 Spike Protein Antigen. AU - Mavrikou,Sophie, AU - Moschopoulou,Georgia, AU - Tsekouras,Vasileios, AU - Kintzios,Spyridon, Y1 - 2020/05/31/ PY - 2020/04/27/received PY - 2020/05/18/revised PY - 2020/05/29/accepted PY - 2020/6/4/entrez PY - 2020/6/4/pubmed PY - 2020/6/19/medline KW - Bioelectric Recognition Assay (BERA) KW - Point-of-Care (POC) KW - S1 spike protein KW - membrane engineering KW - serological assay KW - severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) JF - Sensors (Basel, Switzerland) JO - Sensors (Basel) VL - 20 IS - 11 N2 - One of the key challenges of the recent COVID-19 pandemic is the ability to accurately estimate the number of infected individuals, particularly asymptomatic and/or early-stage patients. We herewith report the proof-of-concept development of a biosensor able to detect the SARS-CoV-2 S1 spike protein expressed on the surface of the virus. The biosensor is based on membrane-engineered mammalian cells bearing the human chimeric spike S1 antibody. We demonstrate that the attachment of the protein to the membrane-bound antibodies resulted in a selective and considerable change in the cellular bioelectric properties measured by means of a Bioelectric Recognition Assay. The novel biosensor provided results in an ultra-rapid manner (3 min), with a detection limit of 1 fg/mL and a semi-linear range of response between 10 fg and 1 μg/mL. In addition, no cross-reactivity was observed against the SARS-CoV-2 nucleocapsid protein. Furthermore, the biosensor was configured as a ready-to-use platform, including a portable read-out device operated via smartphone/tablet. In this way, we demonstrate that the novel biosensor can be potentially applied for the mass screening of SARS-CoV-2 surface antigens without prior sample processing, therefore offering a possible solution for the timely monitoring and eventual control of the global coronavirus pandemic. SN - 1424-8220 UR - https://www.unboundmedicine.com/medline/citation/32486477/Development_of_a_Portable,_Ultra-Rapid_and_Ultra-Sensitive_Cell-Based_Biosensor_for_the_Direct_Detection_of_the_SARS-CoV-2_S1_Spike_Protein_Antigen L2 - https://www.mdpi.com/resolver?pii=s20113121 DB - PRIME DP - Unbound Medicine ER -