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A potentially implantable enzyme electrode for amperometric measurement of glucose.

Abstract

A membrane limited amperometric enzyme electrode suitable for glucose measurement in biological fluids was developed. The sensor consists of a central platinum wire (0.3 mm) surrounded by a stainless steel tubing (0.8-1.0 mm outer diameter; 2-4 cm length). By successive dipcoating procedures, layers from cellulose acetate, glucose oxidase (crosslinked with glutaraldehyde) and polyurethane are placed on its surface. The platinum is polarized at +700 mV against steel. In vitro results: Electrodes are stable for at least 6 days. They exhibit a linear range extending to 500 mg/dl glucose. Response times are less than 100 sec. The sensors are not dependent on stirring and are relatively insensitive to changes of pH. Dependency of glucose measurement upon dissolved oxygen is negligible at oxygen concentrations above 0.5 mg/l. In vivo results: Preliminary studies in sheep using subcutaneously implanted needles indicate that short term glucose monitoring is feasible.

Authors+Show Affiliations

,

Abteilung für Innere Medizin I, Universität Ulm.

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Source

MeSH

Animals
Biosensing Techniques
Blood Glucose
Blood Glucose Self-Monitoring
Electrochemistry
Electrodes
Glucose
Glucose Oxidase
Prostheses and Implants
Sheep

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

3248792

Citation

TY - JOUR T1 - A potentially implantable enzyme electrode for amperometric measurement of glucose. AU - Kerner,W, AU - Zier,H, AU - Steinbach,G, AU - Brückel,J, AU - Pfeiffer,E F, AU - Weiss,T, AU - Cammann,K, AU - Planck,H, PY - 1988/1/1/pubmed PY - 1988/1/1/medline PY - 1988/1/1/entrez SP - 8 EP - 13 JF - Hormone and metabolic research. Supplement series JO - Horm. Metab. Res. Suppl. VL - 20 N2 - A membrane limited amperometric enzyme electrode suitable for glucose measurement in biological fluids was developed. The sensor consists of a central platinum wire (0.3 mm) surrounded by a stainless steel tubing (0.8-1.0 mm outer diameter; 2-4 cm length). By successive dipcoating procedures, layers from cellulose acetate, glucose oxidase (crosslinked with glutaraldehyde) and polyurethane are placed on its surface. The platinum is polarized at +700 mV against steel. In vitro results: Electrodes are stable for at least 6 days. They exhibit a linear range extending to 500 mg/dl glucose. Response times are less than 100 sec. The sensors are not dependent on stirring and are relatively insensitive to changes of pH. Dependency of glucose measurement upon dissolved oxygen is negligible at oxygen concentrations above 0.5 mg/l. In vivo results: Preliminary studies in sheep using subcutaneously implanted needles indicate that short term glucose monitoring is feasible. SN - 0170-5903 UR - https://www.unboundmedicine.com/medline/citation/3248792/abstract/A_potentially_implantable_enzyme_electrode_for_amperometric_measurement_of_glucose_ L2 - https://www.lens.org/lens/search?q=citation_id:3248792 ER -