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The HD-ZIP IV transcription factor GL2-LIKE regulates male flowering time and fertility in cucumber.
J Exp Bot. 2020 09 19; 71(18):5425-5437.JE

Abstract

Cucumber is dioecious by nature, having both male and female flowers, and is a model system for unisexual flower development. Knowledge related to male flowering is limited, but it is reported to be regulated by transcription factors and hormone signals. Here, we report functional characterization of the cucumber (Cucumis sativus) GL2-LIKE gene, which encodes a homeodomain leucine zipper (HD-ZIP) IV transcription factor that plays an important role in regulating male flower development. Spatial-temporal expression analyses revealed high-level expression of CsGL2-LIKE in the male flower buds and anthers. CsGL2-LIKE is closely related to AtGL2, which is known to play a key role in trichome development. However, ectopic expression of CsGL2-LIKE in Arabidopsis gl2-8 mutant was unable to rescue the gl2-8 phenotype. Interestingly, the silencing of CsGL2-LIKE delayed male flowering by inhibiting the expression of the florigen gene FT and reduced pollen vigor and seed viability. Protein-protein interaction assays showed that CsGL2-LIKE interacts with the jasmonate ZIM domain protein CsJAZ1 to form a HD-ZIP IV-CsJAZ1 complex. Collectively, our study indicates that CsGL2-LIKE regulates male flowering in cucumber, and reveals a novel function of a HD-ZIP IV transcription factor in regulating male flower development of cucumber.

Authors+Show Affiliations

Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, P. R. China. Engineering Research Center of Breeding and Propagation of Horticultural Crops, Ministry of Education, College of Horticulture, China Agricultural University, Beijing, P. R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, P. R. China. Engineering Research Center of Breeding and Propagation of Horticultural Crops, Ministry of Education, College of Horticulture, China Agricultural University, Beijing, P. R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, P. R. China. Engineering Research Center of Breeding and Propagation of Horticultural Crops, Ministry of Education, College of Horticulture, China Agricultural University, Beijing, P. R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, P. R. China. Engineering Research Center of Breeding and Propagation of Horticultural Crops, Ministry of Education, College of Horticulture, China Agricultural University, Beijing, P. R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, P. R. China. Engineering Research Center of Breeding and Propagation of Horticultural Crops, Ministry of Education, College of Horticulture, China Agricultural University, Beijing, P. R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, P. R. China. Engineering Research Center of Breeding and Propagation of Horticultural Crops, Ministry of Education, College of Horticulture, China Agricultural University, Beijing, P. R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, P. R. China. Engineering Research Center of Breeding and Propagation of Horticultural Crops, Ministry of Education, College of Horticulture, China Agricultural University, Beijing, P. R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, P. R. China. Engineering Research Center of Breeding and Propagation of Horticultural Crops, Ministry of Education, College of Horticulture, China Agricultural University, Beijing, P. R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, P. R. China. Engineering Research Center of Breeding and Propagation of Horticultural Crops, Ministry of Education, College of Horticulture, China Agricultural University, Beijing, P. R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, P. R. China. Engineering Research Center of Breeding and Propagation of Horticultural Crops, Ministry of Education, College of Horticulture, China Agricultural University, Beijing, P. R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, P. R. China. Engineering Research Center of Breeding and Propagation of Horticultural Crops, Ministry of Education, College of Horticulture, China Agricultural University, Beijing, P. R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, P. R. China. Engineering Research Center of Breeding and Propagation of Horticultural Crops, Ministry of Education, College of Horticulture, China Agricultural University, Beijing, P. R. China.Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, P. R. China. Engineering Research Center of Breeding and Propagation of Horticultural Crops, Ministry of Education, College of Horticulture, China Agricultural University, Beijing, P. R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32490515

Citation

Cai, Yanling, et al. "The HD-ZIP IV Transcription Factor GL2-LIKE Regulates Male Flowering Time and Fertility in Cucumber." Journal of Experimental Botany, vol. 71, no. 18, 2020, pp. 5425-5437.
Cai Y, Bartholomew ES, Dong M, et al. The HD-ZIP IV transcription factor GL2-LIKE regulates male flowering time and fertility in cucumber. J Exp Bot. 2020;71(18):5425-5437.
Cai, Y., Bartholomew, E. S., Dong, M., Zhai, X., Yin, S., Zhang, Y., Feng, Z., Wu, L., Liu, W., Shan, N., Zhang, X., Ren, H., & Liu, X. (2020). The HD-ZIP IV transcription factor GL2-LIKE regulates male flowering time and fertility in cucumber. Journal of Experimental Botany, 71(18), 5425-5437. https://doi.org/10.1093/jxb/eraa251
Cai Y, et al. The HD-ZIP IV Transcription Factor GL2-LIKE Regulates Male Flowering Time and Fertility in Cucumber. J Exp Bot. 2020 09 19;71(18):5425-5437. PubMed PMID: 32490515.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The HD-ZIP IV transcription factor GL2-LIKE regulates male flowering time and fertility in cucumber. AU - Cai,Yanling, AU - Bartholomew,Ezra S, AU - Dong,Mingming, AU - Zhai,Xuling, AU - Yin,Shuai, AU - Zhang,Yaqi, AU - Feng,Zhongxuan, AU - Wu,Licai, AU - Liu,Wan, AU - Shan,Nan, AU - Zhang,Xiao, AU - Ren,Huazhong, AU - Liu,Xingwang, PY - 2020/03/16/received PY - 2020/05/27/accepted PY - 2020/6/4/pubmed PY - 2021/5/15/medline PY - 2020/6/4/entrez KW - Cucumber KW - HD-ZIP IV transcription factor KW - HD-ZIP IV–CsJAZ1 complex KW - jasmonate KW - jasmonate repressor KW - male flowering SP - 5425 EP - 5437 JF - Journal of experimental botany JO - J Exp Bot VL - 71 IS - 18 N2 - Cucumber is dioecious by nature, having both male and female flowers, and is a model system for unisexual flower development. Knowledge related to male flowering is limited, but it is reported to be regulated by transcription factors and hormone signals. Here, we report functional characterization of the cucumber (Cucumis sativus) GL2-LIKE gene, which encodes a homeodomain leucine zipper (HD-ZIP) IV transcription factor that plays an important role in regulating male flower development. Spatial-temporal expression analyses revealed high-level expression of CsGL2-LIKE in the male flower buds and anthers. CsGL2-LIKE is closely related to AtGL2, which is known to play a key role in trichome development. However, ectopic expression of CsGL2-LIKE in Arabidopsis gl2-8 mutant was unable to rescue the gl2-8 phenotype. Interestingly, the silencing of CsGL2-LIKE delayed male flowering by inhibiting the expression of the florigen gene FT and reduced pollen vigor and seed viability. Protein-protein interaction assays showed that CsGL2-LIKE interacts with the jasmonate ZIM domain protein CsJAZ1 to form a HD-ZIP IV-CsJAZ1 complex. Collectively, our study indicates that CsGL2-LIKE regulates male flowering in cucumber, and reveals a novel function of a HD-ZIP IV transcription factor in regulating male flower development of cucumber. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/32490515/The_HD_ZIP_IV_transcription_factor_GL2_LIKE_regulates_male_flowering_time_and_fertility_in_cucumber_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/eraa251 DB - PRIME DP - Unbound Medicine ER -