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Inhibition kinetics of digestive proteases for Anticarsia gemmatalis.
An Acad Bras Cienc. 2020; 92 Suppl 1:e20180477.AA

Abstract

Anticarsia gemmatalis Hübner, 1818 (Lepidoptera) is a major pest of soybean in the Brazil. It is known that the reduction of proteolytic activity by the ingestion of protease inhibitors reduces digestion and larval development of the insects. Control via inhibition of the digestive enzymes necessitates deeper knowledge of the enzyme kinetics and the characterization of the inhibition kinetics of these proteases, for better understanding of the active centers and action mechanisms of this enzyme. Trypsin-like proteases found in the gut of Anticarsia gemmatalis were purified in a p-aminobenzamidine agarose column. Kinetic characterization showed KM 0.503 mM for the L-BApNA substrate; Vmax= 46.650 nM s-1; Vmax/[E]= 9.256 nM s-1 mg L-1 and Vmax/[E]/KM= 18.402 nM s-1 mg L-1 mM. The Ki values for the inhibitors benzamidine, berenil, SKTI and SBBI were 11.2 µM, 32.4 µM, 0.25 nM and 1.4 nM, respectively, and all revealed linear competitive inhibition. The SKTI showed the greatest inhibition, which makes it a promising subject for future research to manufacture peptide mimetic inhibitors.

Authors+Show Affiliations

Departamento de Bioquímica e Biologia Molecular, Instituto de Biotecnologia Aplicada a Agropecuária/BIOAGRO, Viçosa, MG, Brazil.Departamento de Bioquímica e Biologia Molecular, Instituto de Biotecnologia Aplicada a Agropecuária/BIOAGRO, Viçosa, MG, Brazil.Departamento de Bioquímica e Biologia Molecular, Instituto de Biotecnologia Aplicada a Agropecuária/BIOAGRO, Viçosa, MG, Brazil.Instituto de Ciências Agrárias, Universidade Federal de Viçosa, Rio Paranaíba, MG, Brazil.Departamento de Bioquímica e Biologia Molecular, Instituto de Biotecnologia Aplicada a Agropecuária/BIOAGRO, Viçosa, MG, Brazil.Instituto de Ciências Biológicas, Universidade Federal de Viçosa, Rio Paranaíba, MG, Brazil.Departamento de Entomologia, Instituto de Biotecnologia Aplicada a Agropecuária/ BIOAGRO, Viçosa, MG, Brazil.Departamento de Engenharia de Biossistemas, Universidade Federal de São João Del-Rei, São João Del-Rei, MG, Brazil.Departamento de Bioquímica e Biologia Molecular, Instituto de Biotecnologia Aplicada a Agropecuária/BIOAGRO, Viçosa, MG, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32491140

Citation

Patarroyo-Vargas, Adriana M., et al. "Inhibition Kinetics of Digestive Proteases for Anticarsia Gemmatalis." Anais Da Academia Brasileira De Ciencias, vol. 92 Suppl 1, 2020, pp. e20180477.
Patarroyo-Vargas AM, Cordeiro G, Silva CRD, et al. Inhibition kinetics of digestive proteases for Anticarsia gemmatalis. An Acad Bras Cienc. 2020;92 Suppl 1:e20180477.
Patarroyo-Vargas, A. M., Cordeiro, G., Silva, C. R. D., Silva, C. R. D., Mendonça, E. G., Visôtto, L. E., Zanuncio, J. C., Campos, W. G., & Oliveira, M. G. A. (2020). Inhibition kinetics of digestive proteases for Anticarsia gemmatalis. Anais Da Academia Brasileira De Ciencias, 92 Suppl 1, e20180477. https://doi.org/10.1590/0001-3765202020180477
Patarroyo-Vargas AM, et al. Inhibition Kinetics of Digestive Proteases for Anticarsia Gemmatalis. An Acad Bras Cienc. 2020;92 Suppl 1:e20180477. PubMed PMID: 32491140.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition kinetics of digestive proteases for Anticarsia gemmatalis. AU - Patarroyo-Vargas,Adriana M, AU - Cordeiro,Gláucia, AU - Silva,Carolina R DA, AU - Silva,Camila R DA, AU - Mendonça,Eduardo G, AU - Visôtto,Liliane E, AU - Zanuncio,José C, AU - Campos,Welligton G, AU - Oliveira,Maria Goreti A, Y1 - 2020/06/01/ PY - 2018/05/16/received PY - 2019/03/26/accepted PY - 2020/6/4/entrez PY - 2020/6/4/pubmed PY - 2020/6/18/medline SP - e20180477 EP - e20180477 JF - Anais da Academia Brasileira de Ciencias JO - An Acad Bras Cienc VL - 92 Suppl 1 N2 - Anticarsia gemmatalis Hübner, 1818 (Lepidoptera) is a major pest of soybean in the Brazil. It is known that the reduction of proteolytic activity by the ingestion of protease inhibitors reduces digestion and larval development of the insects. Control via inhibition of the digestive enzymes necessitates deeper knowledge of the enzyme kinetics and the characterization of the inhibition kinetics of these proteases, for better understanding of the active centers and action mechanisms of this enzyme. Trypsin-like proteases found in the gut of Anticarsia gemmatalis were purified in a p-aminobenzamidine agarose column. Kinetic characterization showed KM 0.503 mM for the L-BApNA substrate; Vmax= 46.650 nM s-1; Vmax/[E]= 9.256 nM s-1 mg L-1 and Vmax/[E]/KM= 18.402 nM s-1 mg L-1 mM. The Ki values for the inhibitors benzamidine, berenil, SKTI and SBBI were 11.2 µM, 32.4 µM, 0.25 nM and 1.4 nM, respectively, and all revealed linear competitive inhibition. The SKTI showed the greatest inhibition, which makes it a promising subject for future research to manufacture peptide mimetic inhibitors. SN - 1678-2690 UR - https://www.unboundmedicine.com/medline/citation/32491140/Inhibition_kinetics_of_digestive_proteases_for_Anticarsia_gemmatalis_ DB - PRIME DP - Unbound Medicine ER -