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Evidence for mutations in SARS-CoV-2 Italian isolates potentially affecting virus transmission.
J Med Virol. 2020 10; 92(10):2232-2237.JM

Abstract

Italy is the first western country suffering heavy severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission and disease impact after coronavirus disease-2019 pandemia started in China. Even though the presence of mutations on spike glycoprotein and nucleocapsid in Italian isolates has been reported, the potential impact of these mutations on viral transmission has not been evaluated. We have compared SARS-CoV-2 genome sequences from Italian patients with virus sequences from Chinese patients. We focussed upon three nonsynonymous mutations of genes coding for S(one) and N (two) viral proteins present in Italian isolates and absent in Chinese ones, using various bioinformatics tools. Amino acid analysis and changes in three-dimensional protein structure suggests the mutations reduce protein stability and, particularly for S1 mutation, the enhanced torsional ability of the molecule could favor virus binding to cell receptor(s). This theoretical interpretation awaits experimental and clinical confirmation.

Authors+Show Affiliations

Department of Medical Statistics and Molecular Epidemiology, University Campus Bio-Medico of Rome, Rome, Italy.Department of Medical Biology, Izmir University of Economics, Izmir, Turkey.Deparment of Flavivirus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro - RJ, Brasil.Department of Biochemical Sciences "A. Rossi Fanelli", University of Rome "La Sapienza", Rome, Italy.Department of Clinical Laboratory Science, University Campus Bio-Medico of Rome, Rome, Italy.Department of Biochemical Sciences "A. Rossi Fanelli", University of Rome "La Sapienza", Rome, Italy.Department of Malattie Infettive - Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Rome, Italy. Department of Healthcare Surveillance and Bioethics, Catholic University of Sacred Heart, Rome, Italy.Department of Medical Statistics and Molecular Epidemiology, University Campus Bio-Medico of Rome, Rome, Italy.Department of Genomics, Genetics and Biology, University of Siena, Siena, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32492183

Citation

Benvenuto, Domenico, et al. "Evidence for Mutations in SARS-CoV-2 Italian Isolates Potentially Affecting Virus Transmission." Journal of Medical Virology, vol. 92, no. 10, 2020, pp. 2232-2237.
Benvenuto D, Demir AB, Giovanetti M, et al. Evidence for mutations in SARS-CoV-2 Italian isolates potentially affecting virus transmission. J Med Virol. 2020;92(10):2232-2237.
Benvenuto, D., Demir, A. B., Giovanetti, M., Bianchi, M., Angeletti, S., Pascarella, S., Cauda, R., Ciccozzi, M., & Cassone, A. (2020). Evidence for mutations in SARS-CoV-2 Italian isolates potentially affecting virus transmission. Journal of Medical Virology, 92(10), 2232-2237. https://doi.org/10.1002/jmv.26104
Benvenuto D, et al. Evidence for Mutations in SARS-CoV-2 Italian Isolates Potentially Affecting Virus Transmission. J Med Virol. 2020;92(10):2232-2237. PubMed PMID: 32492183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence for mutations in SARS-CoV-2 Italian isolates potentially affecting virus transmission. AU - Benvenuto,Domenico, AU - Demir,Ayse Banu, AU - Giovanetti,Marta, AU - Bianchi,Martina, AU - Angeletti,Silvia, AU - Pascarella,Stefano, AU - Cauda,Roberto, AU - Ciccozzi,Massimo, AU - Cassone,Antonio, Y1 - 2020/06/19/ PY - 2020/05/13/received PY - 2020/05/28/accepted PY - 2020/6/4/pubmed PY - 2020/12/30/medline PY - 2020/6/4/entrez KW - COVID-19 KW - SARS coronavirus KW - bioinformatics KW - molecular evolution KW - mutation SP - 2232 EP - 2237 JF - Journal of medical virology JO - J Med Virol VL - 92 IS - 10 N2 - Italy is the first western country suffering heavy severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission and disease impact after coronavirus disease-2019 pandemia started in China. Even though the presence of mutations on spike glycoprotein and nucleocapsid in Italian isolates has been reported, the potential impact of these mutations on viral transmission has not been evaluated. We have compared SARS-CoV-2 genome sequences from Italian patients with virus sequences from Chinese patients. We focussed upon three nonsynonymous mutations of genes coding for S(one) and N (two) viral proteins present in Italian isolates and absent in Chinese ones, using various bioinformatics tools. Amino acid analysis and changes in three-dimensional protein structure suggests the mutations reduce protein stability and, particularly for S1 mutation, the enhanced torsional ability of the molecule could favor virus binding to cell receptor(s). This theoretical interpretation awaits experimental and clinical confirmation. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/32492183/Evidence_for_mutations_in_SARS_CoV_2_Italian_isolates_potentially_affecting_virus_transmission_ L2 - https://doi.org/10.1002/jmv.26104 DB - PRIME DP - Unbound Medicine ER -