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Assessing heterogeneity among single embryos and single blastomeres using open microfluidic design.
Sci Adv. 2020 Apr; 6(17):eaay1751.SA

Abstract

The process by which a zygote develops from a single cell into a multicellular organism is poorly understood. Advances are hindered by detection specificity and sensitivity limitations of single-cell protein tools and by challenges in integrating multimodal data. We introduce an open microfluidic tool expressly designed for same-cell phenotypic, protein, and mRNA profiling. We examine difficult-to-study-yet critically important-murine preimplantation embryo stages. In blastomeres dissociated from less well-studied two-cell embryos, we observe no significant GADD45a protein expression heterogeneity, apparent at the four-cell stage. In oocytes, we detect differences in full-length versus truncated DICER-1 mRNA and protein, which are insignificant by the two-cell stage. Single-embryo analyses reveal intraembryonic heterogeneity, differences between embryos of the same fertilization event and between donors, and reductions in the burden of animal sacrifice. Open microfluidic design integrates with existing workflows and opens new avenues for assessing the cellular-to-molecular heterogeneity inherent to preimplantation embryo development.

Authors+Show Affiliations

Department of Bioengineering, University of California Berkeley, Berkeley, CA 94720, USA. The University of California Berkeley and University of California San Francisco Graduate Program in Bioengineering, Berkeley, CA 94720, USA.Division of Cellular and Developmental Biology, Department of Molecular & Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.Department of Bioengineering, University of California Berkeley, Berkeley, CA 94720, USA. The University of California Berkeley and University of California San Francisco Graduate Program in Bioengineering, Berkeley, CA 94720, USA.Division of Cellular and Developmental Biology, Department of Molecular & Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.Department of Bioengineering, University of California Berkeley, Berkeley, CA 94720, USA. The University of California Berkeley and University of California San Francisco Graduate Program in Bioengineering, Berkeley, CA 94720, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32494630

Citation

Rosàs-Canyelles, Elisabet, et al. "Assessing Heterogeneity Among Single Embryos and Single Blastomeres Using Open Microfluidic Design." Science Advances, vol. 6, no. 17, 2020, pp. eaay1751.
Rosàs-Canyelles E, Modzelewski AJ, Geldert A, et al. Assessing heterogeneity among single embryos and single blastomeres using open microfluidic design. Sci Adv. 2020;6(17):eaay1751.
Rosàs-Canyelles, E., Modzelewski, A. J., Geldert, A., He, L., & Herr, A. E. (2020). Assessing heterogeneity among single embryos and single blastomeres using open microfluidic design. Science Advances, 6(17), eaay1751. https://doi.org/10.1126/sciadv.aay1751
Rosàs-Canyelles E, et al. Assessing Heterogeneity Among Single Embryos and Single Blastomeres Using Open Microfluidic Design. Sci Adv. 2020;6(17):eaay1751. PubMed PMID: 32494630.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessing heterogeneity among single embryos and single blastomeres using open microfluidic design. AU - Rosàs-Canyelles,Elisabet, AU - Modzelewski,Andrew J, AU - Geldert,Alisha, AU - He,Lin, AU - Herr,Amy E, Y1 - 2020/04/22/ PY - 2019/05/26/received PY - 2020/01/28/accepted PY - 2020/6/5/entrez PY - 2020/6/5/pubmed PY - 2020/6/5/medline SP - eaay1751 EP - eaay1751 JF - Science advances JO - Sci Adv VL - 6 IS - 17 N2 - The process by which a zygote develops from a single cell into a multicellular organism is poorly understood. Advances are hindered by detection specificity and sensitivity limitations of single-cell protein tools and by challenges in integrating multimodal data. We introduce an open microfluidic tool expressly designed for same-cell phenotypic, protein, and mRNA profiling. We examine difficult-to-study-yet critically important-murine preimplantation embryo stages. In blastomeres dissociated from less well-studied two-cell embryos, we observe no significant GADD45a protein expression heterogeneity, apparent at the four-cell stage. In oocytes, we detect differences in full-length versus truncated DICER-1 mRNA and protein, which are insignificant by the two-cell stage. Single-embryo analyses reveal intraembryonic heterogeneity, differences between embryos of the same fertilization event and between donors, and reductions in the burden of animal sacrifice. Open microfluidic design integrates with existing workflows and opens new avenues for assessing the cellular-to-molecular heterogeneity inherent to preimplantation embryo development. SN - 2375-2548 UR - https://www.unboundmedicine.com/medline/citation/32494630/Assessing_heterogeneity_among_single_embryos_and_single_blastomeres_using_open_microfluidic_design L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32494630/ DB - PRIME DP - Unbound Medicine ER -
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