Tags

Type your tag names separated by a space and hit enter

Actin Gene-Based Molecular Typing of Trichomonas vaginalis Clinical Isolates from the North of Iran.
Acta Parasitol. 2020 Jun 04 [Online ahead of print]AP

Abstract

AIM

The aim of the current study is to evaluate the prevalence of trichomoniasis in men and women in the north of Iran and to find genotypes in the positive clinical specimens based on T. vaginalis actin gene.

MATERIALS AND METHODS

Women's genital (n = 500) and men's urine (n = 1500) samples were collected from the participants referred to clinics in Mazandaran Province, northern Iran, during 2006-2018. In addition, 1500 Pap smear specimens, archived in the Bu Ali Hospital, Sari City, Mazandaran Province, northern Iran, were examined. The specimens were examined based on parasitological methods, nested polymerase chain reaction (PCR), PCR-restriction fragment length polymorphism, and phylogenetic analysis.

RESULTS

Overall, 17 (0.48%) of 3500 specimens were positive by PCR. Total prevalence was 0.55% (n = 2000) for women, of which 500 (1.4%; n = 7) specimens were collected freshly, and 1500 (0.26%; n = 4) were Pap smears. Moreover, six (0.4%) out of 1500 men urine specimens were positive. Overall, genotypes G, E, and I were detected with the prevalence of seven (0.2%), seven (0.2%), and three (0.08%), respectively. There was no significant statistical difference among the prevalence of the detected genotypes (P > 0.05).

CONCLUSION

As a whole, the prevalence of trichomoniasis was low in the studied area in the north of Iran and, most importantly, the genotypes of E, G, and I were distributed among men and women in the province.

Authors+Show Affiliations

Department of Parasitology, Molecular and Cell Biology Research Center, Iranian National Registry Center for Lophomoniasis and Toxoplasmosis, Mazandaran University of Medical Sciences, Sari, Iran.Department of Parasitology, Molecular and Cell Biology Research Center, Iranian National Registry Center for Lophomoniasis and Toxoplasmosis, Mazandaran University of Medical Sciences, Sari, Iran.Student Research Committee, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgān, Iran.Department of Microbiology, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgān, Iran.Diabetes Research Center, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, Sari, Iran.Department of Parasitology, Molecular and Cell Biology Research Center, Iranian National Registry Center for Lophomoniasis and Toxoplasmosis, Mazandaran University of Medical Sciences, Sari, Iran.Department of Parasitology, Molecular and Cell Biology Research Center, Iranian National Registry Center for Lophomoniasis and Toxoplasmosis, Mazandaran University of Medical Sciences, Sari, Iran.Department of Parasitology, Toxoplasmosis Research Center, Iranian National Registry Center for Lophomoniasis and Toxoplasmosis, Farah-Abad Road, P.O. Box: 48471-91971, Sari, Iran. mahdif53@yahoo.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32500506

Citation

Ziaei Hezarjaribi, Hajar, et al. "Actin Gene-Based Molecular Typing of Trichomonas Vaginalis Clinical Isolates From the North of Iran." Acta Parasitologica, 2020.
Ziaei Hezarjaribi H, Taghavi M, Hasanjani Saravi K, et al. Actin Gene-Based Molecular Typing of Trichomonas vaginalis Clinical Isolates from the North of Iran. Acta Parasitol. 2020.
Ziaei Hezarjaribi, H., Taghavi, M., Hasanjani Saravi, K., Faridnia, R., Kalani, H., Mardani, A., Jorjani, O., Hosseinikhah, Z., Rahimi Esboei, B., Gholami, M., & Fakhar, M. (2020). Actin Gene-Based Molecular Typing of Trichomonas vaginalis Clinical Isolates from the North of Iran. Acta Parasitologica. https://doi.org/10.2478/s11686-020-00226-x
Ziaei Hezarjaribi H, et al. Actin Gene-Based Molecular Typing of Trichomonas Vaginalis Clinical Isolates From the North of Iran. Acta Parasitol. 2020 Jun 4; PubMed PMID: 32500506.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Actin Gene-Based Molecular Typing of Trichomonas vaginalis Clinical Isolates from the North of Iran. AU - Ziaei Hezarjaribi,Hajar, AU - Taghavi,Mahbobeh, AU - Hasanjani Saravi,Karan, AU - Faridnia,Roghiyeh, AU - Kalani,Hamed, AU - Mardani,Ahmad, AU - Jorjani,Ogholniaz, AU - Hosseinikhah,Zahra, AU - Rahimi Esboei,Bahman, AU - Gholami,Mehran, AU - Fakhar,Mahdi, Y1 - 2020/06/04/ PY - 2019/09/27/received PY - 2020/05/06/accepted PY - 2020/6/6/entrez KW - Genotyping KW - Iran KW - Prevalence KW - Trichomonas vaginalis JF - Acta parasitologica JO - Acta Parasitol. N2 - AIM: The aim of the current study is to evaluate the prevalence of trichomoniasis in men and women in the north of Iran and to find genotypes in the positive clinical specimens based on T. vaginalis actin gene. MATERIALS AND METHODS: Women's genital (n = 500) and men's urine (n = 1500) samples were collected from the participants referred to clinics in Mazandaran Province, northern Iran, during 2006-2018. In addition, 1500 Pap smear specimens, archived in the Bu Ali Hospital, Sari City, Mazandaran Province, northern Iran, were examined. The specimens were examined based on parasitological methods, nested polymerase chain reaction (PCR), PCR-restriction fragment length polymorphism, and phylogenetic analysis. RESULTS: Overall, 17 (0.48%) of 3500 specimens were positive by PCR. Total prevalence was 0.55% (n = 2000) for women, of which 500 (1.4%; n = 7) specimens were collected freshly, and 1500 (0.26%; n = 4) were Pap smears. Moreover, six (0.4%) out of 1500 men urine specimens were positive. Overall, genotypes G, E, and I were detected with the prevalence of seven (0.2%), seven (0.2%), and three (0.08%), respectively. There was no significant statistical difference among the prevalence of the detected genotypes (P > 0.05). CONCLUSION: As a whole, the prevalence of trichomoniasis was low in the studied area in the north of Iran and, most importantly, the genotypes of E, G, and I were distributed among men and women in the province. SN - 1896-1851 UR - https://www.unboundmedicine.com/medline/citation/32500506/Actin_Gene-Based_Molecular_Typing_of_Trichomonas_vaginalis_Clinical_Isolates_from_the_North_of_Iran L2 - https://dx.doi.org/10.2478/s11686-020-00226-x DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.