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Non-invasive Electroarthrography Measures Load-Induced Cartilage Streaming Potentials via Electrodes Placed on Skin Surrounding an Articular Joint.
Cartilage. 2020 Jun 05 [Online ahead of print]C

Abstract

OBJECTIVE

We aimed to demonstrate that electroarthrography (EAG) measures streaming potentials originating in the cartilage extracellular matrix during load bearing through electrodes adhered to skin surrounding an articular joint.

DESIGN

Equine metacarpophalangeal joints were subjected to simulated physiological loads while (1) replacing synovial fluid with immersion buffers of different electrolyte concentrations and (2) directly degrading cartilage with trypsin.

RESULTS

An inverse relationship between ionic strength and EAG coefficient was detected. Compared to native synovial fluid, EAG coefficients increased (P < 0.05) for 5 of 6 electrodes immersed in 0.1X phosphate-buffered saline (PBS) (0.014 M NaCl), decreased (P < 0.05) for 4 of 6 electrodes in 1X PBS (0.14 M NaCl), and decreased (P < 0.05) for all 6 electrodes in 10X PBS (1.4 M NaCl). This relationship corresponds to similar studies where streaming potentials were directly measured on cartilage. EAG coefficients, obtained after trypsin degradation, were reduced (P < 0.05) in 6 of 8, and 7 of 8 electrodes, during simulated standing and walking, respectively. Trypsin degradation was confirmed by direct cartilage assessments. Streaming potentials, measured by directly contacting cartilage, indicated lower cartilage stiffness (P < 10-5). Unconfined compression data revealed reduced Em, representing proteoglycan matrix stiffness (P = 0.005), no change in Ef, representing collagen network stiffness (P = 0.15), and no change in permeability (P = 0.24). Trypsin depleted proteoglycan as observed by both dimethylmethylene blue assay (P = 0.0005) and safranin-O stained histological sections.

CONCLUSION

These data show that non-invasive EAG detects streaming potentials produced by cartilage during joint compression and has potential to become a diagnostic tool capable of detecting early cartilage degeneration.

Authors+Show Affiliations

Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, Ontario, Canada. Department of Surgery and Department of Laboratory Medicine & Pathobiology, University of Toronto, Toronto, Ontario, Canada.Biomomentum Inc., Laval, Quebec, Canada.Biomomentum Inc., Laval, Quebec, Canada.Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, Ontario, Canada.College of Engineering and Physical Sciences, University of Guelph, Guelph, Ontario, Canada.Biomedical and Electrical Engineering, École Polytechnique, Montréal, Quebec, Canada.Department of Bioengineering, George Mason University, Fairfax, VA, USA.Comparative Orthopaedic Research Laboratory, Department of Clinical Studies, University of Guelph, Guelph, Ontario, Canada.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32500724

Citation

Changoor, Adele, et al. "Non-invasive Electroarthrography Measures Load-Induced Cartilage Streaming Potentials Via Electrodes Placed On Skin Surrounding an Articular Joint." Cartilage, 2020, p. 1947603520928583.
Changoor A, Garon M, Quenneville E, et al. Non-invasive Electroarthrography Measures Load-Induced Cartilage Streaming Potentials via Electrodes Placed on Skin Surrounding an Articular Joint. Cartilage. 2020.
Changoor, A., Garon, M., Quenneville, E., Bull, S. B., Gordon, K., Savard, P., Buschmann, M. D., & Hurtig, M. B. (2020). Non-invasive Electroarthrography Measures Load-Induced Cartilage Streaming Potentials via Electrodes Placed on Skin Surrounding an Articular Joint. Cartilage, 1947603520928583. https://doi.org/10.1177/1947603520928583
Changoor A, et al. Non-invasive Electroarthrography Measures Load-Induced Cartilage Streaming Potentials Via Electrodes Placed On Skin Surrounding an Articular Joint. Cartilage. 2020 Jun 5;1947603520928583. PubMed PMID: 32500724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Non-invasive Electroarthrography Measures Load-Induced Cartilage Streaming Potentials via Electrodes Placed on Skin Surrounding an Articular Joint. AU - Changoor,Adele, AU - Garon,Martin, AU - Quenneville,Eric, AU - Bull,Shelley B, AU - Gordon,Karen, AU - Savard,Pierre, AU - Buschmann,Michael D, AU - Hurtig,Mark B, Y1 - 2020/06/05/ PY - 2020/6/6/entrez KW - biomechanics KW - cartilage degeneration KW - early osteoarthritis KW - electroarthrography KW - electromechanics KW - streaming potential SP - 1947603520928583 EP - 1947603520928583 JF - Cartilage JO - Cartilage N2 - OBJECTIVE: We aimed to demonstrate that electroarthrography (EAG) measures streaming potentials originating in the cartilage extracellular matrix during load bearing through electrodes adhered to skin surrounding an articular joint. DESIGN: Equine metacarpophalangeal joints were subjected to simulated physiological loads while (1) replacing synovial fluid with immersion buffers of different electrolyte concentrations and (2) directly degrading cartilage with trypsin. RESULTS: An inverse relationship between ionic strength and EAG coefficient was detected. Compared to native synovial fluid, EAG coefficients increased (P < 0.05) for 5 of 6 electrodes immersed in 0.1X phosphate-buffered saline (PBS) (0.014 M NaCl), decreased (P < 0.05) for 4 of 6 electrodes in 1X PBS (0.14 M NaCl), and decreased (P < 0.05) for all 6 electrodes in 10X PBS (1.4 M NaCl). This relationship corresponds to similar studies where streaming potentials were directly measured on cartilage. EAG coefficients, obtained after trypsin degradation, were reduced (P < 0.05) in 6 of 8, and 7 of 8 electrodes, during simulated standing and walking, respectively. Trypsin degradation was confirmed by direct cartilage assessments. Streaming potentials, measured by directly contacting cartilage, indicated lower cartilage stiffness (P < 10-5). Unconfined compression data revealed reduced Em, representing proteoglycan matrix stiffness (P = 0.005), no change in Ef, representing collagen network stiffness (P = 0.15), and no change in permeability (P = 0.24). Trypsin depleted proteoglycan as observed by both dimethylmethylene blue assay (P = 0.0005) and safranin-O stained histological sections. CONCLUSION: These data show that non-invasive EAG detects streaming potentials produced by cartilage during joint compression and has potential to become a diagnostic tool capable of detecting early cartilage degeneration. SN - 1947-6043 UR - https://www.unboundmedicine.com/medline/citation/32500724/Non-invasive_Electroarthrography_Measures_Load-Induced_Cartilage_Streaming_Potentials_via_Electrodes_Placed_on_Skin_Surrounding_an_Articular_Joint L2 - https://journals.sagepub.com/doi/10.1177/1947603520928583?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -
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