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Proteomic Analysis of Sri Lanka Echis carinatus Venom: Immunological Cross-Reactivity and Enzyme Neutralization Potency of Indian Polyantivenom.
J Proteome Res. 2020 Jun 23 [Online ahead of print]JP

Abstract

The saw-scaled viper (Echis carinatus carinatus) is a major venomous snake in Sri Lanka (SL) responsible for massive numbers of snakebites on the island; nevertheless, its venom proteome composition has never been explored. The proteome composition of SL E. c. carinatus venom (SL ECV), revealed by tandem mass spectrometry analysis, showed that it is composed of 42 enzymatic and nonenzymatic proteins belonging to 12 snake venom protein families. Snake venom metalloproteases (SVMP) and snaclec comprised the most abundant enzymatic and nonenzymatic proteins, respectively. When the composition of SL ECV was compared to the previously determined venom composition of Southern India ECV (SI ECV), 16 proteins were found in common. The SL ECV proteome composition was correlated with the clinical manifestations and pathophysiology of E. c. carinatus envenomation in SL. Polyvalent antivenom (PAV) raised in equine against the "Big Four" venomous snakes of India is typically exported to SL for snakebite treatment; however, the poor immunological cross-reactivity, partial in vitro neutralization of enzymatic activities, and some pharmacological properties, mostly shown by low molecular mass toxins (25 kDa) of SL ECV by Indian PAVs are major concerns for the effective treatment of ECV envenomation in SL.

Authors+Show Affiliations

Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32501701

Citation

Patra, Aparup, and Ashis K. Mukherjee. "Proteomic Analysis of Sri Lanka Echis Carinatus Venom: Immunological Cross-Reactivity and Enzyme Neutralization Potency of Indian Polyantivenom." Journal of Proteome Research, 2020.
Patra A, Mukherjee AK. Proteomic Analysis of Sri Lanka Echis carinatus Venom: Immunological Cross-Reactivity and Enzyme Neutralization Potency of Indian Polyantivenom. J Proteome Res. 2020.
Patra, A., & Mukherjee, A. K. (2020). Proteomic Analysis of Sri Lanka Echis carinatus Venom: Immunological Cross-Reactivity and Enzyme Neutralization Potency of Indian Polyantivenom. Journal of Proteome Research. https://doi.org/10.1021/acs.jproteome.0c00054
Patra A, Mukherjee AK. Proteomic Analysis of Sri Lanka Echis Carinatus Venom: Immunological Cross-Reactivity and Enzyme Neutralization Potency of Indian Polyantivenom. J Proteome Res. 2020 Jun 23; PubMed PMID: 32501701.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic Analysis of Sri Lanka Echis carinatus Venom: Immunological Cross-Reactivity and Enzyme Neutralization Potency of Indian Polyantivenom. AU - Patra,Aparup, AU - Mukherjee,Ashis K, Y1 - 2020/06/23/ PY - 2020/6/6/pubmed PY - 2020/6/6/medline PY - 2020/6/6/entrez KW - Echis carinatus KW - Sri Lanka snake venom KW - antivenom KW - immunological cross-reactivity KW - pathophysiology of Echis bite KW - proteomics KW - venom neutralization JF - Journal of proteome research JO - J. Proteome Res. N2 - The saw-scaled viper (Echis carinatus carinatus) is a major venomous snake in Sri Lanka (SL) responsible for massive numbers of snakebites on the island; nevertheless, its venom proteome composition has never been explored. The proteome composition of SL E. c. carinatus venom (SL ECV), revealed by tandem mass spectrometry analysis, showed that it is composed of 42 enzymatic and nonenzymatic proteins belonging to 12 snake venom protein families. Snake venom metalloproteases (SVMP) and snaclec comprised the most abundant enzymatic and nonenzymatic proteins, respectively. When the composition of SL ECV was compared to the previously determined venom composition of Southern India ECV (SI ECV), 16 proteins were found in common. The SL ECV proteome composition was correlated with the clinical manifestations and pathophysiology of E. c. carinatus envenomation in SL. Polyvalent antivenom (PAV) raised in equine against the "Big Four" venomous snakes of India is typically exported to SL for snakebite treatment; however, the poor immunological cross-reactivity, partial in vitro neutralization of enzymatic activities, and some pharmacological properties, mostly shown by low molecular mass toxins (25 kDa) of SL ECV by Indian PAVs are major concerns for the effective treatment of ECV envenomation in SL. SN - 1535-3907 UR - https://www.unboundmedicine.com/medline/citation/32501701/Proteomic_analysis_of_Sri_Lanka_Echis_carinatus_venom:_Immunological_cross-reactivity_and_enzyme_neutralization_potency_of_Indian_polyantivenom L2 - https://doi.org/10.1021/acs.jproteome.0c00054 DB - PRIME DP - Unbound Medicine ER -
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