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Alltest rapid lateral flow immunoassays is reliable in diagnosing SARS-CoV-2 infection from 14 days after symptom onset: A prospective single-center study.
J Clin Virol. 2020 08; 129:104473.JC

Abstract

OBJECTIVES

SARS-CoV-2 infection diagnosis is challenging in patients from 2 to 3 weeks after the onset of symptoms, due to the low positivity rate of the PCR. Serologic tests could be complementary to PCR in these situations. The aim of our study was to analyze the diagnostic performance of one serologic rapid test in COVID-19 patients.

METHODS

We evaluated a lateral flow immunoassay (AllTest COVID-19 IgG/IgM) which detects IgG and IgM antibodies. We validated the serologic test using serum samples from 100 negative patients (group 1) and 90 patients with COVID-19 confirmed by PCR (group 2). Then, we prospectively evaluated the test in 61 patients with clinical diagnosis of pneumonia of unknown etiology that were negative for SARS-CoV-2 by PCR (group 3).

RESULTS

All 100 patients from group 1 were negative for the serologic test (specificity = 100 %). Regarding group 2 (PCR-positive), the median time from their symptom onset until testing was 17 days. For these 90 group-2 patients, the test was positive for either IgM or IgG in 58 (overall sensitivity = 64.4 %), and in patients tested 14 days or more after the onset of symptoms, the sensitivity was 88.0 %. Regarding the 61 group-3 patients, median time after symptom onset was also 17 days, and the test was positive in 54 (88.5 % positivity).

CONCLUSIONS

Our study shows that Alltest lateral flow immunoassay is reliable as a complement of PCR to diagnose SARS-CoV-2 infection after 14 days from the onset of symptoms and in patients with pneumonia and negative PCR for SARS-CoV-2.

Authors+Show Affiliations

Departamento De Microbiología Clínica, Hospital Universitario Príncipe De Asturias, Madrid, Spain. Electronic address: felipe.perez.garcia.87@gmail.com.Departamento De Microbiología Clínica, Hospital Universitario Príncipe De Asturias, Madrid, Spain. Electronic address: ramontanoira@hotmail.com.Departamento De Microbiología Clínica, Hospital Universitario Príncipe De Asturias, Madrid, Spain; Departamento De Biomedicina y Biotecnología, Facultad De Medicina, Universidad De Alcalá De Henares, Spain. Electronic address: jromanyk@yahoo.es.Departamento De Microbiología Clínica, Hospital Universitario Príncipe De Asturias, Madrid, Spain. Electronic address: tarroyo26@hotmail.com.Departamento De Microbiología Clínica, Hospital Universitario Príncipe De Asturias, Madrid, Spain. Electronic address: pgherruz@gmail.com.Departamento De Microbiología Clínica, Hospital Universitario Príncipe De Asturias, Madrid, Spain; Departamento De Biomedicina y Biotecnología, Facultad De Medicina, Universidad De Alcalá De Henares, Spain. Electronic address: juan.cuadros@uah.es.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

32504945

Citation

Pérez-García, Felipe, et al. "Alltest Rapid Lateral Flow Immunoassays Is Reliable in Diagnosing SARS-CoV-2 Infection From 14 Days After Symptom Onset: a Prospective Single-center Study." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 129, 2020, p. 104473.
Pérez-García F, Pérez-Tanoira R, Romanyk J, et al. Alltest rapid lateral flow immunoassays is reliable in diagnosing SARS-CoV-2 infection from 14 days after symptom onset: A prospective single-center study. J Clin Virol. 2020;129:104473.
Pérez-García, F., Pérez-Tanoira, R., Romanyk, J., Arroyo, T., Gómez-Herruz, P., & Cuadros-González, J. (2020). Alltest rapid lateral flow immunoassays is reliable in diagnosing SARS-CoV-2 infection from 14 days after symptom onset: A prospective single-center study. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 129, 104473. https://doi.org/10.1016/j.jcv.2020.104473
Pérez-García F, et al. Alltest Rapid Lateral Flow Immunoassays Is Reliable in Diagnosing SARS-CoV-2 Infection From 14 Days After Symptom Onset: a Prospective Single-center Study. J Clin Virol. 2020;129:104473. PubMed PMID: 32504945.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alltest rapid lateral flow immunoassays is reliable in diagnosing SARS-CoV-2 infection from 14 days after symptom onset: A prospective single-center study. AU - Pérez-García,Felipe, AU - Pérez-Tanoira,Ramón, AU - Romanyk,Juan, AU - Arroyo,Teresa, AU - Gómez-Herruz,Peña, AU - Cuadros-González,Juan, Y1 - 2020/05/27/ PY - 2020/05/17/received PY - 2020/05/20/revised PY - 2020/05/25/accepted PY - 2020/6/7/pubmed PY - 2020/8/12/medline PY - 2020/6/7/entrez KW - Alltest KW - COVID-19 KW - Diagnosis KW - Lateral flow immunoassay KW - SARS-CoV-2 KW - Serologic rapid test SP - 104473 EP - 104473 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 129 N2 - OBJECTIVES: SARS-CoV-2 infection diagnosis is challenging in patients from 2 to 3 weeks after the onset of symptoms, due to the low positivity rate of the PCR. Serologic tests could be complementary to PCR in these situations. The aim of our study was to analyze the diagnostic performance of one serologic rapid test in COVID-19 patients. METHODS: We evaluated a lateral flow immunoassay (AllTest COVID-19 IgG/IgM) which detects IgG and IgM antibodies. We validated the serologic test using serum samples from 100 negative patients (group 1) and 90 patients with COVID-19 confirmed by PCR (group 2). Then, we prospectively evaluated the test in 61 patients with clinical diagnosis of pneumonia of unknown etiology that were negative for SARS-CoV-2 by PCR (group 3). RESULTS: All 100 patients from group 1 were negative for the serologic test (specificity = 100 %). Regarding group 2 (PCR-positive), the median time from their symptom onset until testing was 17 days. For these 90 group-2 patients, the test was positive for either IgM or IgG in 58 (overall sensitivity = 64.4 %), and in patients tested 14 days or more after the onset of symptoms, the sensitivity was 88.0 %. Regarding the 61 group-3 patients, median time after symptom onset was also 17 days, and the test was positive in 54 (88.5 % positivity). CONCLUSIONS: Our study shows that Alltest lateral flow immunoassay is reliable as a complement of PCR to diagnose SARS-CoV-2 infection after 14 days from the onset of symptoms and in patients with pneumonia and negative PCR for SARS-CoV-2. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/32504945/Alltest_rapid_lateral_flow_immunoassays_is_reliable_in_diagnosing_SARS_CoV_2_infection_from_14_days_after_symptom_onset:_A_prospective_single_center_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(20)30215-8 DB - PRIME DP - Unbound Medicine ER -