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Systematic identification and functional analysis of potato (Solanum tuberosum L.) bZIP transcription factors and overexpression of potato bZIP transcription factor StbZIP-65 enhances salt tolerance.
Int J Biol Macromol. 2020 Oct 15; 161:155-167.IJ

Abstract

Basic leucine zipper (bZIP) transcription factors play important roles in numerous growth and developmental processes. Potato (Solanum tuberosum L.) is a worldwide important vegetable crop; nevertheless, no systematic identification or functional analysis of the potato bZIP gene family has been reported. In this research, 65 potato bZIPs distributed on 12 potato chromosomes were identified. According to the topology of Arabidopsis and potato bZIP phylogenetic tree, the bZIPs were classified into thirteen groups, designated as A-K, M, and S, with no potato bZIPs included in groups J and M. The bZIPs from the same group shared a conserved exon-intron structure, intron phase, and motif composition. Eighteen potato bZIPs were involved in segmental duplications, and the duplicated gene pairs were under purifying selection. No tandemly duplicated potato bZIP was found. Each potato bZIP promoter contained at least one kind of stress-responsive or stress-related hormone-responsive element. RNA-seq and qRT-PCR analyses revealed different expression patterns of potato bZIPs under abiotic stresses. The overexpression of StbZIP-65 in Arabidopsis enhanced salt tolerance. The StbZIP-65 protein localized in the nucleus. β-Glucuronidase staining showed that promoter activity of StbZIP-65 was induced by exogenous methyl jasmonate. These results may aid in further functional studies of potato bZIP transcription factors.

Authors+Show Affiliations

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China.State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: dongdong-1025@hotmail.com.College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: chenpeter2289@nwafu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32512099

Citation

Zhao, Peng, et al. "Systematic Identification and Functional Analysis of Potato (Solanum Tuberosum L.) bZIP Transcription Factors and Overexpression of Potato bZIP Transcription Factor StbZIP-65 Enhances Salt Tolerance." International Journal of Biological Macromolecules, vol. 161, 2020, pp. 155-167.
Zhao P, Ye M, Wang R, et al. Systematic identification and functional analysis of potato (Solanum tuberosum L.) bZIP transcription factors and overexpression of potato bZIP transcription factor StbZIP-65 enhances salt tolerance. Int J Biol Macromol. 2020;161:155-167.
Zhao, P., Ye, M., Wang, R., Wang, D., & Chen, Q. (2020). Systematic identification and functional analysis of potato (Solanum tuberosum L.) bZIP transcription factors and overexpression of potato bZIP transcription factor StbZIP-65 enhances salt tolerance. International Journal of Biological Macromolecules, 161, 155-167. https://doi.org/10.1016/j.ijbiomac.2020.06.032
Zhao P, et al. Systematic Identification and Functional Analysis of Potato (Solanum Tuberosum L.) bZIP Transcription Factors and Overexpression of Potato bZIP Transcription Factor StbZIP-65 Enhances Salt Tolerance. Int J Biol Macromol. 2020 Oct 15;161:155-167. PubMed PMID: 32512099.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Systematic identification and functional analysis of potato (Solanum tuberosum L.) bZIP transcription factors and overexpression of potato bZIP transcription factor StbZIP-65 enhances salt tolerance. AU - Zhao,Peng, AU - Ye,Minghui, AU - Wang,Ruoqiu, AU - Wang,Dongdong, AU - Chen,Qin, Y1 - 2020/06/06/ PY - 2020/05/07/received PY - 2020/06/03/accepted PY - 2020/6/9/pubmed PY - 2021/3/27/medline PY - 2020/6/9/entrez KW - Potato KW - Salt tolerance KW - bZIP transcription factor SP - 155 EP - 167 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 161 N2 - Basic leucine zipper (bZIP) transcription factors play important roles in numerous growth and developmental processes. Potato (Solanum tuberosum L.) is a worldwide important vegetable crop; nevertheless, no systematic identification or functional analysis of the potato bZIP gene family has been reported. In this research, 65 potato bZIPs distributed on 12 potato chromosomes were identified. According to the topology of Arabidopsis and potato bZIP phylogenetic tree, the bZIPs were classified into thirteen groups, designated as A-K, M, and S, with no potato bZIPs included in groups J and M. The bZIPs from the same group shared a conserved exon-intron structure, intron phase, and motif composition. Eighteen potato bZIPs were involved in segmental duplications, and the duplicated gene pairs were under purifying selection. No tandemly duplicated potato bZIP was found. Each potato bZIP promoter contained at least one kind of stress-responsive or stress-related hormone-responsive element. RNA-seq and qRT-PCR analyses revealed different expression patterns of potato bZIPs under abiotic stresses. The overexpression of StbZIP-65 in Arabidopsis enhanced salt tolerance. The StbZIP-65 protein localized in the nucleus. β-Glucuronidase staining showed that promoter activity of StbZIP-65 was induced by exogenous methyl jasmonate. These results may aid in further functional studies of potato bZIP transcription factors. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/32512099/Systematic_identification_and_functional_analysis_of_potato__Solanum_tuberosum_L___bZIP_transcription_factors_and_overexpression_of_potato_bZIP_transcription_factor_StbZIP_65_enhances_salt_tolerance_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(20)33472-3 DB - PRIME DP - Unbound Medicine ER -