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Evaluation of rapid diagnosis of novel coronavirus disease (COVID-19) using loop-mediated isothermal amplification.
J Clin Virol. 2020 08; 129:104446.JC

Abstract

With the rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), there is an urgent need for more rapid and simple detection technologies at the forefront of medical care worldwide. In this study, we evaluated the effectiveness of the Loopamp® 2019-SARSCoV-2 Detection Reagent Kit, which uses loop-mediated isothermal amplification (LAMP) technology. In this protocol, cDNA is synthesized from SARS-CoV-2 RNA using reverse transcriptase, followed by DNA amplification under isothermal conditions in one step. The RT-LAMP test kit amplified the targeted RNA of a SARS-CoV-2 isolate with a detection limit of 1.0 × 101 copies/μL, which was comparable to the detection sensitivity of quantitative reverse transcription PCR (RT-qPCR). Comparison with the results of RT-qPCR for 76 nasopharyngeal swab samples from patients with suspected COVID-19 showed a sensitivity of 100 % and a specificity of 97.6 %. In the 24 RNA specimens derived from febrile Japanese patients with or without influenza A, no amplification was observed using RT-LAMP. RT-LAMP could be a simple and easy-to-use diagnostic tool for the detection of SARS-CoV-2.

Authors+Show Affiliations

Department of Clinical Laboratory, Saitama Medical University Hospital, Saitama, Japan.Department of Clinical Laboratory, Saitama Medical University Hospital, Saitama, Japan.Department of Clinical Laboratory, Saitama Medical University Hospital, Saitama, Japan.Department of Infectious Disease and Infection Control, Saitama Medical University, Saitama, Japan.Department of Infectious Disease and Infection Control, Saitama Medical University, Saitama, Japan.Department of Infectious Disease and Infection Control, Saitama Medical University, Saitama, Japan.Department of Clinical Laboratory, Saitama Medical University Hospital, Saitama, Japan.Department of Clinical Laboratory, Saitama Medical University Hospital, Saitama, Japan.Department of Infectious Disease and Infection Control, Saitama Medical University, Saitama, Japan.Department of Clinical Laboratory, Saitama Medical University Hospital, Saitama, Japan; Department of Laboratory Medicine, Saitama Medical University, Saitama, Japan. Electronic address: t_maeda@saitama-med.ac.jp.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

32512376

Citation

Kitagawa, Yutaro, et al. "Evaluation of Rapid Diagnosis of Novel Coronavirus Disease (COVID-19) Using Loop-mediated Isothermal Amplification." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 129, 2020, p. 104446.
Kitagawa Y, Orihara Y, Kawamura R, et al. Evaluation of rapid diagnosis of novel coronavirus disease (COVID-19) using loop-mediated isothermal amplification. J Clin Virol. 2020;129:104446.
Kitagawa, Y., Orihara, Y., Kawamura, R., Imai, K., Sakai, J., Tarumoto, N., Matsuoka, M., Takeuchi, S., Maesaki, S., & Maeda, T. (2020). Evaluation of rapid diagnosis of novel coronavirus disease (COVID-19) using loop-mediated isothermal amplification. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 129, 104446. https://doi.org/10.1016/j.jcv.2020.104446
Kitagawa Y, et al. Evaluation of Rapid Diagnosis of Novel Coronavirus Disease (COVID-19) Using Loop-mediated Isothermal Amplification. J Clin Virol. 2020;129:104446. PubMed PMID: 32512376.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of rapid diagnosis of novel coronavirus disease (COVID-19) using loop-mediated isothermal amplification. AU - Kitagawa,Yutaro, AU - Orihara,Yuta, AU - Kawamura,Rieko, AU - Imai,Kazuo, AU - Sakai,Jun, AU - Tarumoto,Norihito, AU - Matsuoka,Masaru, AU - Takeuchi,Shinichi, AU - Maesaki,Shigefumi, AU - Maeda,Takuya, Y1 - 2020/05/21/ PY - 2020/04/25/received PY - 2020/05/08/revised PY - 2020/05/14/accepted PY - 2020/6/9/pubmed PY - 2020/8/12/medline PY - 2020/6/9/entrez KW - COVID-19 KW - LAMP KW - RT-qPCR KW - SARS-CoV-2 SP - 104446 EP - 104446 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J. Clin. Virol. VL - 129 N2 - With the rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), there is an urgent need for more rapid and simple detection technologies at the forefront of medical care worldwide. In this study, we evaluated the effectiveness of the Loopamp® 2019-SARSCoV-2 Detection Reagent Kit, which uses loop-mediated isothermal amplification (LAMP) technology. In this protocol, cDNA is synthesized from SARS-CoV-2 RNA using reverse transcriptase, followed by DNA amplification under isothermal conditions in one step. The RT-LAMP test kit amplified the targeted RNA of a SARS-CoV-2 isolate with a detection limit of 1.0 × 101 copies/μL, which was comparable to the detection sensitivity of quantitative reverse transcription PCR (RT-qPCR). Comparison with the results of RT-qPCR for 76 nasopharyngeal swab samples from patients with suspected COVID-19 showed a sensitivity of 100 % and a specificity of 97.6 %. In the 24 RNA specimens derived from febrile Japanese patients with or without influenza A, no amplification was observed using RT-LAMP. RT-LAMP could be a simple and easy-to-use diagnostic tool for the detection of SARS-CoV-2. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/32512376/Evaluation_of_rapid_diagnosis_of_novel_coronavirus_disease__COVID_19__using_loop_mediated_isothermal_amplification_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(20)30188-8 DB - PRIME DP - Unbound Medicine ER -