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The role of RHOA signaling in trophectoderm cell-fate decision in cattle.
Biochem Biophys Res Commun. 2020 Aug 06; 528(4):713-718.BB

Abstract

Mammalian blastocysts are composed of two distinct cell lineages, namely the inner cell mass (ICM) and trophectoderm (TE). TE cells that give rise to the embryonic placenta are marked by an exclusive expression of the key determinant transcription factor, CDX2. Although Hippo signaling pathway is known to be responsible for this TE-specific expression of CDX2, the upstream regulator of this pathway in mammalian embryos is still controversial. In the present study, the involvement of the small molecular G protein, RHOA, in TE cell-fate decision in cattle was investigated. Inhibition of RHOA by the specific inhibitor, C3 transferase (C3), severely impaired the blastocyst formation. Further, C3 treatment significantly decreased the number of blastomeres with nuclearized YAP1, the prominent effector of Hippo pathway. An artificial isolation of ICM cells from blastocysts followed by the continuing culture to regenerate TE cells was conducted and showed that TE re-emergence from the isolated ICM is governed by Hippo pathway and suppressed by C3 treatment like that observed in developing embryos. Finally, the long-term exposure to C3 suggests the presence of alternative regulators of CDX2 expression other than RHOA signaling because there were still CDX2-positive cells after C3 treatment. These results demonstrated that RHOA signaling plays a significant role in TE cell-fate decision by regulating Hippo pathway in cattle.

Authors+Show Affiliations

Laboratory of Animal Genetics and Reproduction, Research Faculty of Agriculture, Hokkaido University, Kita-ku, Kita 9, Nishi 9, Sapporo, 060-8589, Japan. Electronic address: nana7mi3@shinshi-u.ac.jp.Laboratory of Animal Genetics and Reproduction, Research Faculty of Agriculture, Hokkaido University, Kita-ku, Kita 9, Nishi 9, Sapporo, 060-8589, Japan. Electronic address: akizwa-h@anim.agr.hokudai.ac.jp.Laboratory of Animal Genetics and Reproduction, Research Faculty of Agriculture, Hokkaido University, Kita-ku, Kita 9, Nishi 9, Sapporo, 060-8589, Japan. Electronic address: s_iisaka@anim.agr.hokudai.ac.jp.Laboratory of Animal Genetics and Reproduction, Research Faculty of Agriculture, Hokkaido University, Kita-ku, Kita 9, Nishi 9, Sapporo, 060-8589, Japan. Electronic address: hbai@anim.agr.hokudai.ac.jp.Laboratory of Animal Genetics and Reproduction, Research Faculty of Agriculture, Hokkaido University, Kita-ku, Kita 9, Nishi 9, Sapporo, 060-8589, Japan. Electronic address: mmasashi@anim.agr.hokudai.ac.jp.Laboratory of Animal Genetics and Reproduction, Research Faculty of Agriculture, Hokkaido University, Kita-ku, Kita 9, Nishi 9, Sapporo, 060-8589, Japan. Electronic address: k-hara@anim.agr.hokudai.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32513530

Citation

Kohri, Nanami, et al. "The Role of RHOA Signaling in Trophectoderm Cell-fate Decision in Cattle." Biochemical and Biophysical Research Communications, vol. 528, no. 4, 2020, pp. 713-718.
Kohri N, Akizawa H, Iisaka S, et al. The role of RHOA signaling in trophectoderm cell-fate decision in cattle. Biochem Biophys Res Commun. 2020;528(4):713-718.
Kohri, N., Akizawa, H., Iisaka, S., Bai, H., Takahashi, M., & Kawahara, M. (2020). The role of RHOA signaling in trophectoderm cell-fate decision in cattle. Biochemical and Biophysical Research Communications, 528(4), 713-718. https://doi.org/10.1016/j.bbrc.2020.05.210
Kohri N, et al. The Role of RHOA Signaling in Trophectoderm Cell-fate Decision in Cattle. Biochem Biophys Res Commun. 2020 Aug 6;528(4):713-718. PubMed PMID: 32513530.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of RHOA signaling in trophectoderm cell-fate decision in cattle. AU - Kohri,Nanami, AU - Akizawa,Hiroki, AU - Iisaka,Sakie, AU - Bai,Hanako, AU - Takahashi,Masashi, AU - Kawahara,Manabu, Y1 - 2020/06/06/ PY - 2020/05/22/received PY - 2020/05/28/accepted PY - 2020/6/10/pubmed PY - 2020/6/10/medline PY - 2020/6/10/entrez KW - Blastocyst KW - Bovine KW - Differentiation KW - Hippo signaling pathway KW - RHOA activity KW - Trophectoderm SP - 713 EP - 718 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 528 IS - 4 N2 - Mammalian blastocysts are composed of two distinct cell lineages, namely the inner cell mass (ICM) and trophectoderm (TE). TE cells that give rise to the embryonic placenta are marked by an exclusive expression of the key determinant transcription factor, CDX2. Although Hippo signaling pathway is known to be responsible for this TE-specific expression of CDX2, the upstream regulator of this pathway in mammalian embryos is still controversial. In the present study, the involvement of the small molecular G protein, RHOA, in TE cell-fate decision in cattle was investigated. Inhibition of RHOA by the specific inhibitor, C3 transferase (C3), severely impaired the blastocyst formation. Further, C3 treatment significantly decreased the number of blastomeres with nuclearized YAP1, the prominent effector of Hippo pathway. An artificial isolation of ICM cells from blastocysts followed by the continuing culture to regenerate TE cells was conducted and showed that TE re-emergence from the isolated ICM is governed by Hippo pathway and suppressed by C3 treatment like that observed in developing embryos. Finally, the long-term exposure to C3 suggests the presence of alternative regulators of CDX2 expression other than RHOA signaling because there were still CDX2-positive cells after C3 treatment. These results demonstrated that RHOA signaling plays a significant role in TE cell-fate decision by regulating Hippo pathway in cattle. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/32513530/The_role_of_RHOA_signaling_in_trophectoderm_cell-fate_decision_in_cattle L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(20)31159-1 DB - PRIME DP - Unbound Medicine ER -
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