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Six long noncoding RNAs as potentially biomarkers involved in competitive endogenous RNA of hepatocellular carcinoma.
Clin Exp Med. 2020 Aug; 20(3):437-447.CE

Abstract

To investigate lncRNAs acting as competing endogenous RNAs (ceRNAs) involved in oncogenesis and progression of HCC. Different expressed lncRNAs, microRNAs, and mRNAs (DElncRNAs, DEmiRNAs, DEmRNAs), downloaded from The Cancer Genome Atlas (TCGA) database, were identified by edgeR package. CeRNA network was constructed based on miRcode, TargetScan, and miRTarBase. Target DEmRNAs were annotated by KEGG pathway and GO analysis. Negatively correlated lncRNA-miRNA pairs were analyzed by Pearson correlation coefficient, simultaneously, overall survival (OS) were evaluated. The expression of these lncRNAs were examined in HCC cell lines and tissues through qRT-PCR. 1070 DElncRNAs, 147 DEmiRNAs and 1993 DEmRNAs were acquired. CeRNA network was successfully established, including 27 lncRNAs, 5 miRNAs, and 30 mRNAs significantly correlated with OS. The DEmRNAs were significantly enriched in "Cell Cycle" and "pathways in cancer". Six lncRNAs and 2 miRNAs were negatively correlated. These lncRNAs were validated by qRT-PCR. These observations will provide a novel perspective to elucidate HCC pathogenesis.

Authors+Show Affiliations

Laboratory Medicine Center, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, 210011, People's Republic of China. State Key Lab of Reproductive Medicine, Jiangsu Key Laboratory of Pathogen Biology, Department of Pathogen Biology and Immunology, Center for Global Health, Nanjing Medical University, Nanjing, 211166, People's Republic of China.Laboratory Medicine Center, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, 210011, People's Republic of China.Laboratory Medicine Center, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, 210011, People's Republic of China.Laboratory Medicine Center, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, 210011, People's Republic of China.Laboratory Medicine Center, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, 210011, People's Republic of China.Laboratory Medicine Center, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, 210011, People's Republic of China.Laboratory Medicine Center, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, 210011, People's Republic of China.State Key Lab of Reproductive Medicine, Jiangsu Key Laboratory of Pathogen Biology, Department of Pathogen Biology and Immunology, Center for Global Health, Nanjing Medical University, Nanjing, 211166, People's Republic of China. chuansu@njmu.edu.cn.Laboratory Medicine Center, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, 210011, People's Republic of China. zhuyf@njmu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32514710

Citation

Qu, Lili, et al. "Six Long Noncoding RNAs as Potentially Biomarkers Involved in Competitive Endogenous RNA of Hepatocellular Carcinoma." Clinical and Experimental Medicine, vol. 20, no. 3, 2020, pp. 437-447.
Qu L, Cai X, Xu J, et al. Six long noncoding RNAs as potentially biomarkers involved in competitive endogenous RNA of hepatocellular carcinoma. Clin Exp Med. 2020;20(3):437-447.
Qu, L., Cai, X., Xu, J., Wei, X., Qu, X., Sun, L., Gong, L., Su, C., & Zhu, Y. (2020). Six long noncoding RNAs as potentially biomarkers involved in competitive endogenous RNA of hepatocellular carcinoma. Clinical and Experimental Medicine, 20(3), 437-447. https://doi.org/10.1007/s10238-020-00634-3
Qu L, et al. Six Long Noncoding RNAs as Potentially Biomarkers Involved in Competitive Endogenous RNA of Hepatocellular Carcinoma. Clin Exp Med. 2020;20(3):437-447. PubMed PMID: 32514710.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Six long noncoding RNAs as potentially biomarkers involved in competitive endogenous RNA of hepatocellular carcinoma. AU - Qu,Lili, AU - Cai,Xiaoxiao, AU - Xu,Junwen, AU - Wei,Xiaowei, AU - Qu,Xiaojun, AU - Sun,Li, AU - Gong,Lailing, AU - Su,Chuan, AU - Zhu,Yefei, Y1 - 2020/06/09/ PY - 2020/03/12/received PY - 2020/05/06/accepted PY - 2020/6/10/pubmed PY - 2020/6/10/medline PY - 2020/6/10/entrez KW - Biomarkers KW - Hepatocellular carcinoma KW - ceRNAs KW - lncRNAs SP - 437 EP - 447 JF - Clinical and experimental medicine JO - Clin. Exp. Med. VL - 20 IS - 3 N2 - To investigate lncRNAs acting as competing endogenous RNAs (ceRNAs) involved in oncogenesis and progression of HCC. Different expressed lncRNAs, microRNAs, and mRNAs (DElncRNAs, DEmiRNAs, DEmRNAs), downloaded from The Cancer Genome Atlas (TCGA) database, were identified by edgeR package. CeRNA network was constructed based on miRcode, TargetScan, and miRTarBase. Target DEmRNAs were annotated by KEGG pathway and GO analysis. Negatively correlated lncRNA-miRNA pairs were analyzed by Pearson correlation coefficient, simultaneously, overall survival (OS) were evaluated. The expression of these lncRNAs were examined in HCC cell lines and tissues through qRT-PCR. 1070 DElncRNAs, 147 DEmiRNAs and 1993 DEmRNAs were acquired. CeRNA network was successfully established, including 27 lncRNAs, 5 miRNAs, and 30 mRNAs significantly correlated with OS. The DEmRNAs were significantly enriched in "Cell Cycle" and "pathways in cancer". Six lncRNAs and 2 miRNAs were negatively correlated. These lncRNAs were validated by qRT-PCR. These observations will provide a novel perspective to elucidate HCC pathogenesis. SN - 1591-9528 UR - https://www.unboundmedicine.com/medline/citation/32514710/Six_long_noncoding_RNAs_as_potentially_biomarkers_involved_in_competitive_endogenous_RNA_of_hepatocellular_carcinoma_ L2 - https://dx.doi.org/10.1007/s10238-020-00634-3 DB - PRIME DP - Unbound Medicine ER -
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