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GABAA Receptor/STEP61 Signaling Pathway May Be Involved in Emulsified Isoflurane Anesthesia in Rats.
Int J Mol Sci. 2020 Jun 07; 21(11)IJ

Abstract

(1)

Background:

Emulsified isoflurane (EISO) is a type of intravenous anesthetic. How emulsified isoflurane works in the brain is still unclear. The aim of this study was to explore whether epigenetic mechanisms affect anesthesia and to evaluate the anesthetic effects of emulsified isoflurane in rats. (2)

Methods:

Rats were randomly divided into four groups (n = 8/group): The tail vein was injected with normal saline 0.1 mL·kg-1·min-1for the control (Con) group, with intralipid for the fat emulsion (FE) group, with EISO at 60 mg·kg-1·min-1 for the high-concentration (HD) group, and 45 mg·kg-1·min-1 for the low-concentration (LD) group. The consciousness state, motor function of limbs, and response to nociceptive stimulus were observed after drug administration. (3)

Results:

Using real-time polymerase chain reaction (PCR) to assess the promoter methylation of ion channel proteins in the cerebral cortex of rats anesthetized by EISO, we demonstrated that the change in the promoters' methylation of the coding genes for gamma-aminobutyric acid A receptor α1 subunit (GABAAα1), N-methyl-D-aspartate receptor subunit 1 (NMDAR1), and mu opioid receptor 1 (OPRM1) was accompanied by the change in messenger ribonucleic acid (mRNA) and protein expression by these genes. (4)

Conclusion:

These data suggest that the epigenetic factors' modulation might offer a novel approach to explore the anesthetic mechanism of EISO.

Authors+Show Affiliations

No affiliation info availableNo affiliation info availableDepartment of Veterinary Clinical Science, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.Department of Veterinary Clinical Science, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32517358

Citation

Zhao, Xingkai, et al. "GABAA Receptor/STEP61 Signaling Pathway May Be Involved in Emulsified Isoflurane Anesthesia in Rats." International Journal of Molecular Sciences, vol. 21, no. 11, 2020.
Zhao X, Chang G, Cheng Y, et al. GABAA Receptor/STEP61 Signaling Pathway May Be Involved in Emulsified Isoflurane Anesthesia in Rats. Int J Mol Sci. 2020;21(11).
Zhao, X., Chang, G., Cheng, Y., & Zhou, Z. (2020). GABAA Receptor/STEP61 Signaling Pathway May Be Involved in Emulsified Isoflurane Anesthesia in Rats. International Journal of Molecular Sciences, 21(11). https://doi.org/10.3390/ijms21114078
Zhao X, et al. GABAA Receptor/STEP61 Signaling Pathway May Be Involved in Emulsified Isoflurane Anesthesia in Rats. Int J Mol Sci. 2020 Jun 7;21(11) PubMed PMID: 32517358.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GABAA Receptor/STEP61 Signaling Pathway May Be Involved in Emulsified Isoflurane Anesthesia in Rats. AU - Zhao,Xingkai, AU - Chang,Guangjun, AU - Cheng,Yan, AU - Zhou,Zhenlei, Y1 - 2020/06/07/ PY - 2020/05/17/received PY - 2020/06/04/revised PY - 2020/06/05/accepted PY - 2020/6/11/entrez PY - 2020/6/11/pubmed PY - 2021/2/13/medline KW - DNA methylation KW - emulsified isoflurane (EISO) KW - epigenetic KW - general anesthesia KW - ion channel protein JF - International journal of molecular sciences JO - Int J Mol Sci VL - 21 IS - 11 N2 - (1) Background: Emulsified isoflurane (EISO) is a type of intravenous anesthetic. How emulsified isoflurane works in the brain is still unclear. The aim of this study was to explore whether epigenetic mechanisms affect anesthesia and to evaluate the anesthetic effects of emulsified isoflurane in rats. (2) Methods: Rats were randomly divided into four groups (n = 8/group): The tail vein was injected with normal saline 0.1 mL·kg-1·min-1for the control (Con) group, with intralipid for the fat emulsion (FE) group, with EISO at 60 mg·kg-1·min-1 for the high-concentration (HD) group, and 45 mg·kg-1·min-1 for the low-concentration (LD) group. The consciousness state, motor function of limbs, and response to nociceptive stimulus were observed after drug administration. (3) Results: Using real-time polymerase chain reaction (PCR) to assess the promoter methylation of ion channel proteins in the cerebral cortex of rats anesthetized by EISO, we demonstrated that the change in the promoters' methylation of the coding genes for gamma-aminobutyric acid A receptor α1 subunit (GABAAα1), N-methyl-D-aspartate receptor subunit 1 (NMDAR1), and mu opioid receptor 1 (OPRM1) was accompanied by the change in messenger ribonucleic acid (mRNA) and protein expression by these genes. (4) Conclusion: These data suggest that the epigenetic factors' modulation might offer a novel approach to explore the anesthetic mechanism of EISO. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/32517358/GABAA_Receptor/STEP61_Signaling_Pathway_May_Be_Involved_in_Emulsified_Isoflurane_Anesthesia_in_Rats_ L2 - https://www.mdpi.com/resolver?pii=ijms21114078 DB - PRIME DP - Unbound Medicine ER -