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Immunoinformatics-guided designing of epitope-based subunit vaccines against the SARS Coronavirus-2 (SARS-CoV-2).
Immunobiology. 2020 05; 225(3):151955.I

Abstract

SARS Coronavirus-2 (SARS-CoV-2) pandemic has become a global issue which has raised the concern of scientific community to design and discover a counter-measure against this deadly virus. So far, the pandemic has caused the death of hundreds of thousands of people upon infection and spreading. To date, no effective vaccine is available which can combat the infection caused by this virus. Therefore, this study was conducted to design possible epitope-based subunit vaccines against the SARS-CoV-2 virus using the approaches of reverse vaccinology and immunoinformatics. Upon continual computational experimentation, three possible vaccine constructs were designed and one vaccine construct was selected as the best vaccine based on molecular docking study which is supposed to effectively act against the SARS-CoV-2. Thereafter, the molecular dynamics simulation and in silico codon adaptation experiments were carried out in order to check biological stability and find effective mass production strategy of the selected vaccine. This study should contribute to uphold the present efforts of the researches to secure a definitive preventative measure against this lethal disease.

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Authors+Show Affiliations

Sarkar B
Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Jahangirnagar University, Dhaka, Bangladesh. Electronic address: sarkarbishajit@gmail.com.
Ullah MA
Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Jahangirnagar University, Dhaka, Bangladesh.
Johora FT
Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Jahangirnagar University, Dhaka, Bangladesh.
Taniya MA
Department of Microbiology, School of Life Sciences, Independent University, Dhaka, Bangladesh.
Araf Y
Department of Genetic Engineering and Biotechnology, School of Life Sciences, Shahjalal University of Science and Technology, Sylhet, Bangladesh.

MeSH

Amino Acid SequenceBetacoronavirusCOVID-19COVID-19 VaccinesComputational BiologyCoronavirus InfectionsDisease ProgressionEpitopesEpitopes, B-LymphocyteEpitopes, T-LymphocyteHLA AntigensHost-Pathogen InteractionsHumansImmunogenicity, VaccineMolecular Docking SimulationPandemicsPlasmidsPneumonia, ViralProtein ConformationReverse GeneticsSARS-CoV-2Sequence AlignmentVaccines, SubunitViral ProteinsViral Vaccines

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32517882

Citation

Sarkar, Bishajit, et al. "Immunoinformatics-guided Designing of Epitope-based Subunit Vaccines Against the SARS Coronavirus-2 (SARS-CoV-2)." Immunobiology, vol. 225, no. 3, 2020, p. 151955.
Sarkar B, Ullah MA, Johora FT, et al. Immunoinformatics-guided designing of epitope-based subunit vaccines against the SARS Coronavirus-2 (SARS-CoV-2). Immunobiology. 2020;225(3):151955.
Sarkar, B., Ullah, M. A., Johora, F. T., Taniya, M. A., & Araf, Y. (2020). Immunoinformatics-guided designing of epitope-based subunit vaccines against the SARS Coronavirus-2 (SARS-CoV-2). Immunobiology, 225(3), 151955. https://doi.org/10.1016/j.imbio.2020.151955
Sarkar B, et al. Immunoinformatics-guided Designing of Epitope-based Subunit Vaccines Against the SARS Coronavirus-2 (SARS-CoV-2). Immunobiology. 2020;225(3):151955. PubMed PMID: 32517882.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunoinformatics-guided designing of epitope-based subunit vaccines against the SARS Coronavirus-2 (SARS-CoV-2). AU - Sarkar,Bishajit, AU - Ullah,Md Asad, AU - Johora,Fatema Tuz, AU - Taniya,Masuma Afrin, AU - Araf,Yusha, Y1 - 2020/05/11/ PY - 2020/03/29/received PY - 2020/05/02/accepted PY - 2020/6/11/entrez PY - 2020/6/11/pubmed PY - 2020/6/24/medline KW - COVID-19 KW - Coronavirus KW - Immunoinformatics KW - Reverse vaccinology KW - SARS-COV-2 KW - Vaccine SP - 151955 EP - 151955 JF - Immunobiology JO - Immunobiology VL - 225 IS - 3 N2 - SARS Coronavirus-2 (SARS-CoV-2) pandemic has become a global issue which has raised the concern of scientific community to design and discover a counter-measure against this deadly virus. So far, the pandemic has caused the death of hundreds of thousands of people upon infection and spreading. To date, no effective vaccine is available which can combat the infection caused by this virus. Therefore, this study was conducted to design possible epitope-based subunit vaccines against the SARS-CoV-2 virus using the approaches of reverse vaccinology and immunoinformatics. Upon continual computational experimentation, three possible vaccine constructs were designed and one vaccine construct was selected as the best vaccine based on molecular docking study which is supposed to effectively act against the SARS-CoV-2. Thereafter, the molecular dynamics simulation and in silico codon adaptation experiments were carried out in order to check biological stability and find effective mass production strategy of the selected vaccine. This study should contribute to uphold the present efforts of the researches to secure a definitive preventative measure against this lethal disease. SN - 1878-3279 UR - https://www.unboundmedicine.com/medline/citation/32517882/Immunoinformatics_guided_designing_of_epitope_based_subunit_vaccines_against_the_SARS_Coronavirus_2__SARS_CoV_2__ DB - PRIME DP - Unbound Medicine ER -