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Hydrolyzable tannins from Poincianella (Caesalpinia) microphylla fruits: Metabolite profiling and anti-Trichomonas vaginalis activity.
Food Res Int. 2020 Aug; 134:109236.FR

Abstract

Poincianella (Caesalpinia) microphylla fractions were analyzed by LC-DAD-MS and by statistical tools to determine the possible bioactive compounds against T. vaginalis. Tannins enriched-fractions toxicity (M5 and M10) were analyzed in in vitro against human red cells and in in vivo model of Galleria mellonella. Twenty-six compounds were detected from the P. microphylla fractions. The main compounds identified were hydrolyzable tannins (gallotanins and ellagitannis), such as O-digalloyl hexoside, O-digalloyl HHDP -hexoside, tri-O-galloyl HHDP-hexoside, O-galloyl HHDP-DHHDP-hexoside and their isomers. In addition, procyanidin dimer, epicatechin, ellagic acid and O-(digalloyl) quinic acid were also identified. Based on univariate statistical analyses, stronger correlations with the anti-T. vaginalis properties were observed for the compounds 7 (O-galloyl HHDP-DHHDP-hexoside - Geraniin isomer), 3 (O-digalloyl HHDP -hexoside - mallotinic acid), 2 (O-digalloyl) quinic acid), 1 (O-digalloyl hexoside) and 9 (unknown). Tannins enriched-fractions (M5 and M10) presented anti-Trichomonas activity (IC50 70.41 µg/mL and 142.1 µg/mL, respectively) and no toxicity in the in vivo model of G. mellonella. This innovative approach allowed us to identify likely bioactive compounds in the extracts, although the mechanism(s) underlying anti-trichomonal activity encompass a complex trait.

Authors+Show Affiliations

Centro de Biotecnologia do Estado do Rio Grande do Sul, Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.Laboratório de Produtos Naturais e Espectrometria de Massas (LAPNEM), Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.Laboratório de Produtos Naturais e Espectrometria de Massas (LAPNEM), Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.Departamento de Ciências Básicas da Saúde. Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil.Centro de Ciências Biológicas e Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.Centro de Biotecnologia do Estado do Rio Grande do Sul, Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil; Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil. Electronic address: alexandre.macedo@ufrgs.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32517934

Citation

Silva, L N., et al. "Hydrolyzable Tannins From Poincianella (Caesalpinia) Microphylla Fruits: Metabolite Profiling and anti-Trichomonas Vaginalis Activity." Food Research International (Ottawa, Ont.), vol. 134, 2020, p. 109236.
Silva LN, Rigo GV, Silva DB, et al. Hydrolyzable tannins from Poincianella (Caesalpinia) microphylla fruits: Metabolite profiling and anti-Trichomonas vaginalis activity. Food Res Int. 2020;134:109236.
Silva, L. N., Rigo, G. V., Silva, D. B., Carollo, C. A., Trentin, D. S., Silva, M. V., Tasca, T., & Macedo, A. J. (2020). Hydrolyzable tannins from Poincianella (Caesalpinia) microphylla fruits: Metabolite profiling and anti-Trichomonas vaginalis activity. Food Research International (Ottawa, Ont.), 134, 109236. https://doi.org/10.1016/j.foodres.2020.109236
Silva LN, et al. Hydrolyzable Tannins From Poincianella (Caesalpinia) Microphylla Fruits: Metabolite Profiling and anti-Trichomonas Vaginalis Activity. Food Res Int. 2020;134:109236. PubMed PMID: 32517934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydrolyzable tannins from Poincianella (Caesalpinia) microphylla fruits: Metabolite profiling and anti-Trichomonas vaginalis activity. AU - Silva,L N, AU - Rigo,G V, AU - Silva,D B, AU - Carollo,C A, AU - Trentin,D S, AU - Silva,M V, AU - Tasca,T, AU - Macedo,A J, Y1 - 2020/04/15/ PY - 2020/01/14/received PY - 2020/04/09/revised PY - 2020/04/09/accepted PY - 2020/6/11/entrez KW - Caatinga KW - Ellagitannis KW - Gallotanins KW - Metabolomics KW - Trichomoniasis SP - 109236 EP - 109236 JF - Food research international (Ottawa, Ont.) JO - Food Res. Int. VL - 134 N2 - Poincianella (Caesalpinia) microphylla fractions were analyzed by LC-DAD-MS and by statistical tools to determine the possible bioactive compounds against T. vaginalis. Tannins enriched-fractions toxicity (M5 and M10) were analyzed in in vitro against human red cells and in in vivo model of Galleria mellonella. Twenty-six compounds were detected from the P. microphylla fractions. The main compounds identified were hydrolyzable tannins (gallotanins and ellagitannis), such as O-digalloyl hexoside, O-digalloyl HHDP -hexoside, tri-O-galloyl HHDP-hexoside, O-galloyl HHDP-DHHDP-hexoside and their isomers. In addition, procyanidin dimer, epicatechin, ellagic acid and O-(digalloyl) quinic acid were also identified. Based on univariate statistical analyses, stronger correlations with the anti-T. vaginalis properties were observed for the compounds 7 (O-galloyl HHDP-DHHDP-hexoside - Geraniin isomer), 3 (O-digalloyl HHDP -hexoside - mallotinic acid), 2 (O-digalloyl) quinic acid), 1 (O-digalloyl hexoside) and 9 (unknown). Tannins enriched-fractions (M5 and M10) presented anti-Trichomonas activity (IC50 70.41 µg/mL and 142.1 µg/mL, respectively) and no toxicity in the in vivo model of G. mellonella. This innovative approach allowed us to identify likely bioactive compounds in the extracts, although the mechanism(s) underlying anti-trichomonal activity encompass a complex trait. SN - 1873-7145 UR - https://www.unboundmedicine.com/medline/citation/32517934/Hydrolyzable_tannins_from_Poincianella_(Caesalpinia)_microphylla_fruits:_Metabolite_profiling_and_anti-Trichomonas_vaginalis_activity L2 - https://linkinghub.elsevier.com/retrieve/pii/S0963-9969(20)30261-1 DB - PRIME DP - Unbound Medicine ER -
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