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Functional Profile of Antigen Specific CD4+ T Cells in the Immune Response to Phospholipase A1 Allergen from Polybia paulista Venom.
Toxins (Basel). 2020 Jun 08; 12(6)T

Abstract

Insect venom can cause systemic allergic reactions, including anaphylaxis. Improvements in diagnosis and venom immunotherapy (VIT) are based on a better understanding of an immunological response triggered by venom allergens. Previously, we demonstrated that the recombinant phospholipase A1 (rPoly p 1) from Polybia paulista wasp venom induces specific IgE and IgG antibodies in sensitized mice, which recognized the native allergen. Here, we addressed the T cell immune response of rPoly p 1-sensitized BALB/c mice. Cultures of splenocytes were stimulated with Polybia paulista venom extract and the proliferation of CD8+ and CD4+ T cells and the frequency of T regulatory cells (Tregs) populations were assessed by flow cytometry. Cytokines were quantified in cell culture supernatants in ELISA assays. The in vitro stimulation of T cells from sensitized mice induces a significant proliferation of CD4+ T cells, but not of CD8+ T cells. The cytokine pattern showed a high concentration of IFN-γ and IL-6, and no significant differences to IL-4, IL-1β and TGF-β1 production. In addition, the rPoly p 1 group showed a pronounced expansion of CD4+CD25+FoxP3+ and CD4+CD25-FoxP3+ Tregs. rPoly p 1 sensitization induces a Th1/Treg profile in CD4+ T cell subset, suggesting its potential use in wasp venom immunotherapy.

Authors+Show Affiliations

Laboratory of Translational Immunology, Medicine School, FCM, University of Campinas (UNICAMP), Campinas 13083-888, Brazil.Center of the Study of Social Insects, Department of General and Applied Biology, Institute of Biosciences of Rio Claro, Institute of Biosciences of Rio Claro, São Paulo State University, Rio Claro 13500, Brazil.Laboratory of Arthropods Molecular Biology-LBMA-IBRC-UNESP (São Paulo State University), Rio Claro 13506-900, Brazil.Laboratory of Translational Immunology, Medicine School, FCM, University of Campinas (UNICAMP), Campinas 13083-888, Brazil.Laboratory of Arthropods Molecular Biology-LBMA-IBRC-UNESP (São Paulo State University), Rio Claro 13506-900, Brazil. Venoms and Venomous Animal Studies Center-CEVAP, São Paulo State University (UNESP), Botucatu 18610-307, Brazil.Laboratory of Translational Immunology, Medicine School, FCM, University of Campinas (UNICAMP), Campinas 13083-888, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32521656

Citation

Fernandes, Luís Gustavo Romani, et al. "Functional Profile of Antigen Specific CD4+ T Cells in the Immune Response to Phospholipase A1 Allergen From Polybia Paulista Venom." Toxins, vol. 12, no. 6, 2020.
Fernandes LGR, Perez-Riverol A, Bazon ML, et al. Functional Profile of Antigen Specific CD4+ T Cells in the Immune Response to Phospholipase A1 Allergen from Polybia paulista Venom. Toxins (Basel). 2020;12(6).
Fernandes, L. G. R., Perez-Riverol, A., Bazon, M. L., Abram, D. M., Brochetto-Braga, M. R., & Zollner, R. L. (2020). Functional Profile of Antigen Specific CD4+ T Cells in the Immune Response to Phospholipase A1 Allergen from Polybia paulista Venom. Toxins, 12(6). https://doi.org/10.3390/toxins12060379
Fernandes LGR, et al. Functional Profile of Antigen Specific CD4+ T Cells in the Immune Response to Phospholipase A1 Allergen From Polybia Paulista Venom. Toxins (Basel). 2020 Jun 8;12(6) PubMed PMID: 32521656.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional Profile of Antigen Specific CD4+ T Cells in the Immune Response to Phospholipase A1 Allergen from Polybia paulista Venom. AU - Fernandes,Luís Gustavo Romani, AU - Perez-Riverol,Amilcar, AU - Bazon,Murilo Luiz, AU - Abram,Débora Moitinho, AU - Brochetto-Braga,Márcia Regina, AU - Zollner,Ricardo de Lima, Y1 - 2020/06/08/ PY - 2020/03/25/received PY - 2020/04/03/revised PY - 2020/04/06/accepted PY - 2020/6/12/entrez PY - 2020/6/12/pubmed PY - 2020/6/12/medline KW - CD4+ T cells KW - Hymenoptera venom allergy KW - Polybia paulista JF - Toxins JO - Toxins (Basel) VL - 12 IS - 6 N2 - Insect venom can cause systemic allergic reactions, including anaphylaxis. Improvements in diagnosis and venom immunotherapy (VIT) are based on a better understanding of an immunological response triggered by venom allergens. Previously, we demonstrated that the recombinant phospholipase A1 (rPoly p 1) from Polybia paulista wasp venom induces specific IgE and IgG antibodies in sensitized mice, which recognized the native allergen. Here, we addressed the T cell immune response of rPoly p 1-sensitized BALB/c mice. Cultures of splenocytes were stimulated with Polybia paulista venom extract and the proliferation of CD8+ and CD4+ T cells and the frequency of T regulatory cells (Tregs) populations were assessed by flow cytometry. Cytokines were quantified in cell culture supernatants in ELISA assays. The in vitro stimulation of T cells from sensitized mice induces a significant proliferation of CD4+ T cells, but not of CD8+ T cells. The cytokine pattern showed a high concentration of IFN-γ and IL-6, and no significant differences to IL-4, IL-1β and TGF-β1 production. In addition, the rPoly p 1 group showed a pronounced expansion of CD4+CD25+FoxP3+ and CD4+CD25-FoxP3+ Tregs. rPoly p 1 sensitization induces a Th1/Treg profile in CD4+ T cell subset, suggesting its potential use in wasp venom immunotherapy. SN - 2072-6651 UR - https://www.unboundmedicine.com/medline/citation/32521656/Functional_Profile_of_Antigen_Specific_CD4+_T_Cells_in_the_Immune_Response_to_Phospholipase_A1_Allergen_from_Polybia_paulista_Venom L2 - https://www.mdpi.com/resolver?pii=toxins12060379 DB - PRIME DP - Unbound Medicine ER -
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