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Application of the Sea Urchin Embryo Test in Toxicity Evaluation and Effect-Directed Analysis of Wastewater Treatment Plant Effluents.
Environ Sci Technol. 2020 Jul 21; 54(14):8890-8899.ES

Abstract

Sea urchin embryo assay was used to assess general toxicity at four wastewater treatment plant effluents of Biscay (Gorliz, Mungia, Gernika, and Galindo), and within the tested range, all the extracts showed embryo growth inhibition and skeleton malformation activities with EC50 values, in relative enrichment factor units, between 1.1-16.8 and 1.1-8.8, respectively. To identify the causative compounds, effect-directed analysis was successfully applied for the first time using a sea urchin embryo test to the secondary treatment of the Galindo effluent. To this end, two subsequent fractionation steps were performed using C18 (21 fractions) and aminopropyl columns (15 fractions). By this fractionation, the number of features detected by LC-HRMS in the raw sample was drastically reduced from 1500 to 9, and among them, two pesticides (mexacarbate, 17 ng/L, and fenpropidin, 23 ng/L), two antidepressants (amitriptyline, 304 ng/L, and paroxetine, 26 ng/L), and two anthelmintic agents (mebendazole, 65 ng/L, and albendazole, 48 ng/L) could be identified in the two toxic fractions. The artificial mixture of the identified six compounds could explain 79% of the observed effect, with albendazole and paroxetine as the predominant contributors (49% and 49%, respectively) affecting the sea urchin embryogenesis activity.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Science and Technology, 48080 Bilbao, Basque Country, Spain. Research Centre for Experimental Marine Biology and Biotechnology (PIE), University of the Basque Country (UPV/EHU), Areatza z/g, 48620 Plentzia, Basque Country, Spain.UFZ - Helmholtz Centre for Environmental Research, Permoserstr. 15, 04318 Leipzig, Germany.Research Centre for Experimental Marine Biology and Biotechnology (PIE), University of the Basque Country (UPV/EHU), Areatza z/g, 48620 Plentzia, Basque Country, Spain. Department of Zoology and Animal Cell Biology, University of the Basque Country (UPV-EHU), E-48080 Bilbao, Basque Country, Spain.Department of Analytical Chemistry, Faculty of Science and Technology, 48080 Bilbao, Basque Country, Spain. Research Centre for Experimental Marine Biology and Biotechnology (PIE), University of the Basque Country (UPV/EHU), Areatza z/g, 48620 Plentzia, Basque Country, Spain.Department of Analytical Chemistry, Faculty of Science and Technology, 48080 Bilbao, Basque Country, Spain. Research Centre for Experimental Marine Biology and Biotechnology (PIE), University of the Basque Country (UPV/EHU), Areatza z/g, 48620 Plentzia, Basque Country, Spain.Department of Analytical Chemistry, Faculty of Science and Technology, 48080 Bilbao, Basque Country, Spain. Research Centre for Experimental Marine Biology and Biotechnology (PIE), University of the Basque Country (UPV/EHU), Areatza z/g, 48620 Plentzia, Basque Country, Spain.Research Centre for Experimental Marine Biology and Biotechnology (PIE), University of the Basque Country (UPV/EHU), Areatza z/g, 48620 Plentzia, Basque Country, Spain. Department of Zoology and Animal Cell Biology, University of the Basque Country (UPV-EHU), E-48080 Bilbao, Basque Country, Spain.UFZ - Helmholtz Centre for Environmental Research, Permoserstr. 15, 04318 Leipzig, Germany.UFZ - Helmholtz Centre for Environmental Research, Permoserstr. 15, 04318 Leipzig, Germany. Institute for Environmental Research, RWTH Aachen University, 52074 Aachen, Germany.Department of Analytical Chemistry, Faculty of Science and Technology, 48080 Bilbao, Basque Country, Spain. Research Centre for Experimental Marine Biology and Biotechnology (PIE), University of the Basque Country (UPV/EHU), Areatza z/g, 48620 Plentzia, Basque Country, Spain.Department of Analytical Chemistry, Faculty of Science and Technology, 48080 Bilbao, Basque Country, Spain. Research Centre for Experimental Marine Biology and Biotechnology (PIE), University of the Basque Country (UPV/EHU), Areatza z/g, 48620 Plentzia, Basque Country, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32525664

Citation

Mijangos, Leire, et al. "Application of the Sea Urchin Embryo Test in Toxicity Evaluation and Effect-Directed Analysis of Wastewater Treatment Plant Effluents." Environmental Science & Technology, vol. 54, no. 14, 2020, pp. 8890-8899.
Mijangos L, Krauss M, de Miguel L, et al. Application of the Sea Urchin Embryo Test in Toxicity Evaluation and Effect-Directed Analysis of Wastewater Treatment Plant Effluents. Environ Sci Technol. 2020;54(14):8890-8899.
Mijangos, L., Krauss, M., de Miguel, L., Ziarrusta, H., Olivares, M., Zuloaga, O., Izagirre, U., Schulze, T., Brack, W., Prieto, A., & Etxebarria, N. (2020). Application of the Sea Urchin Embryo Test in Toxicity Evaluation and Effect-Directed Analysis of Wastewater Treatment Plant Effluents. Environmental Science & Technology, 54(14), 8890-8899. https://doi.org/10.1021/acs.est.0c01504
Mijangos L, et al. Application of the Sea Urchin Embryo Test in Toxicity Evaluation and Effect-Directed Analysis of Wastewater Treatment Plant Effluents. Environ Sci Technol. 2020 Jul 21;54(14):8890-8899. PubMed PMID: 32525664.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of the Sea Urchin Embryo Test in Toxicity Evaluation and Effect-Directed Analysis of Wastewater Treatment Plant Effluents. AU - Mijangos,Leire, AU - Krauss,Martin, AU - de Miguel,Laura, AU - Ziarrusta,Haizea, AU - Olivares,Maitane, AU - Zuloaga,Olatz, AU - Izagirre,Urtzi, AU - Schulze,Tobias, AU - Brack,Werner, AU - Prieto,Ailette, AU - Etxebarria,Nestor, Y1 - 2020/06/28/ PY - 2020/6/12/pubmed PY - 2020/6/12/medline PY - 2020/6/12/entrez SP - 8890 EP - 8899 JF - Environmental science & technology JO - Environ. Sci. Technol. VL - 54 IS - 14 N2 - Sea urchin embryo assay was used to assess general toxicity at four wastewater treatment plant effluents of Biscay (Gorliz, Mungia, Gernika, and Galindo), and within the tested range, all the extracts showed embryo growth inhibition and skeleton malformation activities with EC50 values, in relative enrichment factor units, between 1.1-16.8 and 1.1-8.8, respectively. To identify the causative compounds, effect-directed analysis was successfully applied for the first time using a sea urchin embryo test to the secondary treatment of the Galindo effluent. To this end, two subsequent fractionation steps were performed using C18 (21 fractions) and aminopropyl columns (15 fractions). By this fractionation, the number of features detected by LC-HRMS in the raw sample was drastically reduced from 1500 to 9, and among them, two pesticides (mexacarbate, 17 ng/L, and fenpropidin, 23 ng/L), two antidepressants (amitriptyline, 304 ng/L, and paroxetine, 26 ng/L), and two anthelmintic agents (mebendazole, 65 ng/L, and albendazole, 48 ng/L) could be identified in the two toxic fractions. The artificial mixture of the identified six compounds could explain 79% of the observed effect, with albendazole and paroxetine as the predominant contributors (49% and 49%, respectively) affecting the sea urchin embryogenesis activity. SN - 1520-5851 UR - https://www.unboundmedicine.com/medline/citation/32525664/Application_of_the_sea_urchin_embryo_test_in_toxicity_evaluation_and_effect_directed_analysis_of_wastewater_treatment_plant_effluents L2 - https://doi.org/10.1021/acs.est.0c01504 DB - PRIME DP - Unbound Medicine ER -
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