Tags

Type your tag names separated by a space and hit enter

Rapid detection of novel coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by reverse transcription-loop-mediated isothermal amplification.
PLoS One. 2020; 15(6):e0234682.Plos

Abstract

Novel Corona virus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2 or 2019-nCoV), and the subsequent disease caused by the virus (coronavirus disease 2019 or COVID-19), is an emerging global health concern that requires a rapid diagnostic test. Quantitative reverse transcription PCR (qRT-PCR) is currently the standard for SARS-CoV-2 detection; however, Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) may allow for faster and cheaper field based testing at point-of-risk. The objective of this study was to develop a rapid screening diagnostic test that could be completed in 30-45 minutes. Simulated patient samples were generated by spiking serum, urine, saliva, oropharyngeal swabs, and nasopharyngeal swabs with a portion of the SARS-CoV-2 nucleic sequence. RNA isolated from nasopharyngeal swabs collected from actual COVID-19 patients was also tested. The samples were tested using RT-LAMP as well as by conventional qRT-PCR. Specificity of the RT-LAMP was evaluated by also testing against other related coronaviruses. RT-LAMP specifically detected SARS-CoV-2 in both simulated patient samples and clinical specimens. This test was performed in 30-45 minutes. This approach could be used for monitoring of exposed individuals or potentially aid with screening efforts in the field and potential ports of entry.

Authors+Show Affiliations

Department of Urology, Beaumont Health System, Royal Oak, Michigan, United States of America. Oakland University William Beaumont School of Medicine, Rochester Hills, Michigan, United States of America.Department of Urology, Beaumont Health System, Royal Oak, Michigan, United States of America.Department of Urology, Beaumont Health System, Royal Oak, Michigan, United States of America.Department of Urology, Beaumont Health System, Royal Oak, Michigan, United States of America. Oakland University William Beaumont School of Medicine, Rochester Hills, Michigan, United States of America.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32530929

Citation

Lamb, Laura E., et al. "Rapid Detection of Novel coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) By Reverse Transcription-loop-mediated Isothermal Amplification." PloS One, vol. 15, no. 6, 2020, pp. e0234682.
Lamb LE, Bartolone SN, Ward E, et al. Rapid detection of novel coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by reverse transcription-loop-mediated isothermal amplification. PLoS ONE. 2020;15(6):e0234682.
Lamb, L. E., Bartolone, S. N., Ward, E., & Chancellor, M. B. (2020). Rapid detection of novel coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by reverse transcription-loop-mediated isothermal amplification. PloS One, 15(6), e0234682. https://doi.org/10.1371/journal.pone.0234682
Lamb LE, et al. Rapid Detection of Novel coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) By Reverse Transcription-loop-mediated Isothermal Amplification. PLoS ONE. 2020;15(6):e0234682. PubMed PMID: 32530929.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid detection of novel coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by reverse transcription-loop-mediated isothermal amplification. AU - Lamb,Laura E, AU - Bartolone,Sarah N, AU - Ward,Elijah, AU - Chancellor,Michael B, Y1 - 2020/06/12/ PY - 2020/03/09/received PY - 2020/06/01/accepted PY - 2020/6/13/entrez PY - 2020/6/13/pubmed PY - 2020/6/23/medline SP - e0234682 EP - e0234682 JF - PloS one JO - PLoS ONE VL - 15 IS - 6 N2 - Novel Corona virus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2 or 2019-nCoV), and the subsequent disease caused by the virus (coronavirus disease 2019 or COVID-19), is an emerging global health concern that requires a rapid diagnostic test. Quantitative reverse transcription PCR (qRT-PCR) is currently the standard for SARS-CoV-2 detection; however, Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) may allow for faster and cheaper field based testing at point-of-risk. The objective of this study was to develop a rapid screening diagnostic test that could be completed in 30-45 minutes. Simulated patient samples were generated by spiking serum, urine, saliva, oropharyngeal swabs, and nasopharyngeal swabs with a portion of the SARS-CoV-2 nucleic sequence. RNA isolated from nasopharyngeal swabs collected from actual COVID-19 patients was also tested. The samples were tested using RT-LAMP as well as by conventional qRT-PCR. Specificity of the RT-LAMP was evaluated by also testing against other related coronaviruses. RT-LAMP specifically detected SARS-CoV-2 in both simulated patient samples and clinical specimens. This test was performed in 30-45 minutes. This approach could be used for monitoring of exposed individuals or potentially aid with screening efforts in the field and potential ports of entry. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/32530929/Rapid_detection_of_novel_coronavirus/Severe_Acute_Respiratory_Syndrome_Coronavirus_2__SARS_CoV_2__by_reverse_transcription_loop_mediated_isothermal_amplification_ L2 - http://dx.plos.org/10.1371/journal.pone.0234682 DB - PRIME DP - Unbound Medicine ER -