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Intestinal mir-794 responds to nanopolystyrene by linking insulin and p38 MAPK signaling pathways in nematode Caenorhabditis elegans.
Ecotoxicol Environ Saf. 2020 Sep 15; 201:110857.EE

Abstract

Caenorhabditis elegans is sensitive to toxicity of environmental pollutants. The alteration in expression of mir-794, a microRNA (miRNA) molecule, mediated a protective response to nanopolystyene (100 nm) at predicted environmental concentration (1 μg/L) in nematodes. However, the underlying molecular basis for mir-794 function in regulating the response to nanopolystyrene remains largely unclear. In this study, we found that intestinal overexpression of mir-794 caused the susceptibility to nanopolystyrene toxicity, suggesting that mir-794 acted in the intestine to regulate the response to nanopolystyrene. Intestinal overexpression of mir-794 further decreased the expressions of daf-16 encoding a FOXO transcriptional factor in insulin signaling pathway, skn-1 encoding a Nrf transcriptional factor in p38 MAPK signaling pathway, and mdt-15 encoding a lipid metabolic sensor acting downstream of SKN-1 in nanopolystyrene exposed nematodes. Meanwhile, intestinal overexpression of mir-794 could suppress the resistance of nematodes overexpressing intestinal daf-16, skn-1, or mdt-15 containing the corresponding 3' untranslated region (3' UTR) to nanopolystyrene toxicity. Therefore, DAF-16, SKN-1, and MDT-15 acted as the downstream targets of intestinal mir-794 to regulate the response to nanopolystyrene. In the intestine, DAF-16 functioned synergistically with SKN-1 or MDT-15 to regulate the response to nanopolystyrene. Our results suggested that the intestinal mir-794 provided an important epigenetic regulation mechanism to control the response to nanopolystyrene by linking insulin and p38 MAPK signaling pathways in nematodes.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Qiu Y
Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School, Southeast University, Nanjing, 210009, China.
Liu Y
Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School, Southeast University, Nanjing, 210009, China.
Li Y
School of Public Health, Southeast University, Nanjing, 210009, China. Electronic address: lyh1216@126.com.
Wang D
Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School, Southeast University, Nanjing, 210009, China; Shenzhen Ruipuxun Academy for Stem Cell & Regenerative Medicine, Shenzhen, 518122, China; Guangdong Provincial Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, 510632, China. Electronic address: dayongw@seu.edu.cn.

MeSH

AnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsEpigenesis, GeneticGene Expression RegulationInsulinIntestinesLipidsMAP Kinase Signaling SystemMicroRNAsNanoparticlesPolystyrenesSoil PollutantsTranscription Factorsp38 Mitogen-Activated Protein Kinases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32534332

Citation

Qiu, Yuexiu, et al. "Intestinal Mir-794 Responds to Nanopolystyrene By Linking Insulin and P38 MAPK Signaling Pathways in Nematode Caenorhabditis Elegans." Ecotoxicology and Environmental Safety, vol. 201, 2020, p. 110857.
Qiu Y, Liu Y, Li Y, et al. Intestinal mir-794 responds to nanopolystyrene by linking insulin and p38 MAPK signaling pathways in nematode Caenorhabditis elegans. Ecotoxicol Environ Saf. 2020;201:110857.
Qiu, Y., Liu, Y., Li, Y., & Wang, D. (2020). Intestinal mir-794 responds to nanopolystyrene by linking insulin and p38 MAPK signaling pathways in nematode Caenorhabditis elegans. Ecotoxicology and Environmental Safety, 201, 110857. https://doi.org/10.1016/j.ecoenv.2020.110857
Qiu Y, et al. Intestinal Mir-794 Responds to Nanopolystyrene By Linking Insulin and P38 MAPK Signaling Pathways in Nematode Caenorhabditis Elegans. Ecotoxicol Environ Saf. 2020 Sep 15;201:110857. PubMed PMID: 32534332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intestinal mir-794 responds to nanopolystyrene by linking insulin and p38 MAPK signaling pathways in nematode Caenorhabditis elegans. AU - Qiu,Yuexiu, AU - Liu,Yaqi, AU - Li,Yunhui, AU - Wang,Dayong, Y1 - 2020/06/10/ PY - 2020/04/02/received PY - 2020/05/31/revised PY - 2020/06/01/accepted PY - 2020/6/14/pubmed PY - 2020/8/8/medline PY - 2020/6/14/entrez KW - Caenorhabdis elegans KW - Epigenetic regulation KW - Intestine KW - Nanopolystyrene KW - mir-794 SP - 110857 EP - 110857 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 201 N2 - Caenorhabditis elegans is sensitive to toxicity of environmental pollutants. The alteration in expression of mir-794, a microRNA (miRNA) molecule, mediated a protective response to nanopolystyene (100 nm) at predicted environmental concentration (1 μg/L) in nematodes. However, the underlying molecular basis for mir-794 function in regulating the response to nanopolystyrene remains largely unclear. In this study, we found that intestinal overexpression of mir-794 caused the susceptibility to nanopolystyrene toxicity, suggesting that mir-794 acted in the intestine to regulate the response to nanopolystyrene. Intestinal overexpression of mir-794 further decreased the expressions of daf-16 encoding a FOXO transcriptional factor in insulin signaling pathway, skn-1 encoding a Nrf transcriptional factor in p38 MAPK signaling pathway, and mdt-15 encoding a lipid metabolic sensor acting downstream of SKN-1 in nanopolystyrene exposed nematodes. Meanwhile, intestinal overexpression of mir-794 could suppress the resistance of nematodes overexpressing intestinal daf-16, skn-1, or mdt-15 containing the corresponding 3' untranslated region (3' UTR) to nanopolystyrene toxicity. Therefore, DAF-16, SKN-1, and MDT-15 acted as the downstream targets of intestinal mir-794 to regulate the response to nanopolystyrene. In the intestine, DAF-16 functioned synergistically with SKN-1 or MDT-15 to regulate the response to nanopolystyrene. Our results suggested that the intestinal mir-794 provided an important epigenetic regulation mechanism to control the response to nanopolystyrene by linking insulin and p38 MAPK signaling pathways in nematodes. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/32534332/Intestinal_mir_794_responds_to_nanopolystyrene_by_linking_insulin_and_p38_MAPK_signaling_pathways_in_nematode_Caenorhabditis_elegans_ DB - PRIME DP - Unbound Medicine ER -