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The modulatory role of prime identified compounds in the bioactive fraction of Homalium zeylanicum in high-fat diet fed-streptozotocin-induced type 2 diabetic rats.
J Ethnopharmacol. 2020 Oct 05; 260:113099.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Homalium zeylanicum (Gardner) Benth. is a medicinal plant traditionally used in controlling diabetes which thus far has been assessed by the authors only to a very limited extent.

PURPOSE

To fill the research gap in the literature review, we investigated the antihyperglycemic effects of hydro alcohol fraction of bark of H. zeylanicum (HAHZB) by modulating oxidative stress and inflammation in high-fat diet fed-streptozotocin (HFD/STZ)-induced type-2 diabetic rats.

MATERIALS AND METHODS

To understand the antioxidant capacity of HAHZB, oxygen radical absorbance capacity (ORAC) and cell-based antioxidant protection in erythrocytes (CAP-e) were performed. GC-MS/MS analysis was performed to assess the bioactive components in HAHZB. HFD/STZ-induced diabetic rats were treated orally with HAHZB (300 and 400 mg/kg) for 28 days. After the end of the experiment, marker profiling and histopathological observation of blood and pancreas were examined. The study also highlights interaction between diabetes, oxidative stress and inflammation by examining the increased pro-inflammatory cytokines e.g. TNF-α and C-reactive protein (CRP) promotes DNA damage e.g. oxidation of 8-hydroxy-2-deoxyguanosine (8-OHdG) in chronic hyperglycaemia.

RESULTS

In ex vivo cellular antioxidant capacity of -CAP-e and ORAC assays, HAHZB showed remarkable free radical scavenging ability in a dose dependent manner. GC-MS/MS analysis identified 28 no. of compounds and out of which, oleic acid (1.03%), ethyl tridecanoate (11.77%), phytol (1.29), 9,12-octadecadienoic acid, methyl ester, (E,E)-(5.97%), stigmasterol (1.30%) and β-sitosterol (2.86%) have antioxidant, anti-inflammatory and anti-diabetic activities. HAHZB 400 mg/kg significantly (p < 0.001) improved the lipid profile (TC: 74.66 ± 0.59, HDL-C: 22.08 ± 0.46, LDL-C: 38.06 ± 0.69, and TG: 171.92 ± 1.01 mg/dL) as well as restoring antidiabetic markers (SG: 209.62 ± 1.05 mg/dL, SI: 15.07 ± 0.11 μIU/mL, HOMA-IR: 7.79 ± 0.04 %, and HbA1C: 8.93 ± 0.03 %) and renal functional markers (Tg: 291.26 ± 0.57 pg/mL, BUN: 23.79 ± 0.14 mg/dL, and Cr: 1.34 ± 0.04 mg/dL) in diabetic rats. Oxidative stress markers of pancreas (MDA: 3.65 ± 0.17 nM TBARS /mg protein, SOD: 3.14 ± 0.28 U/mg protein, CAT: 7.88 ± 0.23 U/mg protein, GSH: 12.63 ± 0.28 µM/g of tissue) were restored to normal as evidenced by histological architecture of pancreatic islet cells. The increased level of pro-inflammatory cytokines and oxidative DNA damage were significantly restored (TNF-α: 54.48 ± 3.19 pg/mL, CRP: 440.22 ± 7.86 ng/mL, and 8-OHdG: 63.65 ± 1.84 ng/mL) by HAHZB in diabetic rats.

CONCLUSION

The present findings confirm that the presence of bioactive compounds in HAHZB exert therapeutic protective effect by decreasing oxidative, inflammation and pancreatic β-cell damage in oxidative stress induced diabetic rats.

Authors+Show Affiliations

Regional Plant Resource Centre, Medicinal & Aromatic Plant Division, Forest & Environment Department, Govt. of Odisha, Nayapalli, Bhubaneswar, 751015, India.Regional Plant Resource Centre, Medicinal & Aromatic Plant Division, Forest & Environment Department, Govt. of Odisha, Nayapalli, Bhubaneswar, 751015, India.Regional Plant Resource Centre, Medicinal & Aromatic Plant Division, Forest & Environment Department, Govt. of Odisha, Nayapalli, Bhubaneswar, 751015, India.Regional Plant Resource Centre, Medicinal & Aromatic Plant Division, Forest & Environment Department, Govt. of Odisha, Nayapalli, Bhubaneswar, 751015, India.Regional Plant Resource Centre, Medicinal & Aromatic Plant Division, Forest & Environment Department, Govt. of Odisha, Nayapalli, Bhubaneswar, 751015, India. Electronic address: atish_kumar1976@yahoo.co.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32535241

Citation

Rout, Deeptimayee, et al. "The Modulatory Role of Prime Identified Compounds in the Bioactive Fraction of Homalium Zeylanicum in High-fat Diet Fed-streptozotocin-induced Type 2 Diabetic Rats." Journal of Ethnopharmacology, vol. 260, 2020, p. 113099.
Rout D, Chandra Dash U, Kanhar S, et al. The modulatory role of prime identified compounds in the bioactive fraction of Homalium zeylanicum in high-fat diet fed-streptozotocin-induced type 2 diabetic rats. J Ethnopharmacol. 2020;260:113099.
Rout, D., Chandra Dash, U., Kanhar, S., Swain, S. K., & Sahoo, A. K. (2020). The modulatory role of prime identified compounds in the bioactive fraction of Homalium zeylanicum in high-fat diet fed-streptozotocin-induced type 2 diabetic rats. Journal of Ethnopharmacology, 260, 113099. https://doi.org/10.1016/j.jep.2020.113099
Rout D, et al. The Modulatory Role of Prime Identified Compounds in the Bioactive Fraction of Homalium Zeylanicum in High-fat Diet Fed-streptozotocin-induced Type 2 Diabetic Rats. J Ethnopharmacol. 2020 Oct 5;260:113099. PubMed PMID: 32535241.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The modulatory role of prime identified compounds in the bioactive fraction of Homalium zeylanicum in high-fat diet fed-streptozotocin-induced type 2 diabetic rats. AU - Rout,Deeptimayee, AU - Chandra Dash,Umesh, AU - Kanhar,Satish, AU - Swain,Sandeep Kumar, AU - Sahoo,Atish Kumar, Y1 - 2020/06/11/ PY - 2020/04/22/received PY - 2020/06/06/revised PY - 2020/06/07/accepted PY - 2020/6/15/pubmed PY - 2021/2/26/medline PY - 2020/6/15/entrez KW - 8-OHdG KW - CAP-e KW - CRP KW - Homalium zeylanicum KW - ORAC KW - TNF-α SP - 113099 EP - 113099 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 260 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Homalium zeylanicum (Gardner) Benth. is a medicinal plant traditionally used in controlling diabetes which thus far has been assessed by the authors only to a very limited extent. PURPOSE: To fill the research gap in the literature review, we investigated the antihyperglycemic effects of hydro alcohol fraction of bark of H. zeylanicum (HAHZB) by modulating oxidative stress and inflammation in high-fat diet fed-streptozotocin (HFD/STZ)-induced type-2 diabetic rats. MATERIALS AND METHODS: To understand the antioxidant capacity of HAHZB, oxygen radical absorbance capacity (ORAC) and cell-based antioxidant protection in erythrocytes (CAP-e) were performed. GC-MS/MS analysis was performed to assess the bioactive components in HAHZB. HFD/STZ-induced diabetic rats were treated orally with HAHZB (300 and 400 mg/kg) for 28 days. After the end of the experiment, marker profiling and histopathological observation of blood and pancreas were examined. The study also highlights interaction between diabetes, oxidative stress and inflammation by examining the increased pro-inflammatory cytokines e.g. TNF-α and C-reactive protein (CRP) promotes DNA damage e.g. oxidation of 8-hydroxy-2-deoxyguanosine (8-OHdG) in chronic hyperglycaemia. RESULTS: In ex vivo cellular antioxidant capacity of -CAP-e and ORAC assays, HAHZB showed remarkable free radical scavenging ability in a dose dependent manner. GC-MS/MS analysis identified 28 no. of compounds and out of which, oleic acid (1.03%), ethyl tridecanoate (11.77%), phytol (1.29), 9,12-octadecadienoic acid, methyl ester, (E,E)-(5.97%), stigmasterol (1.30%) and β-sitosterol (2.86%) have antioxidant, anti-inflammatory and anti-diabetic activities. HAHZB 400 mg/kg significantly (p < 0.001) improved the lipid profile (TC: 74.66 ± 0.59, HDL-C: 22.08 ± 0.46, LDL-C: 38.06 ± 0.69, and TG: 171.92 ± 1.01 mg/dL) as well as restoring antidiabetic markers (SG: 209.62 ± 1.05 mg/dL, SI: 15.07 ± 0.11 μIU/mL, HOMA-IR: 7.79 ± 0.04 %, and HbA1C: 8.93 ± 0.03 %) and renal functional markers (Tg: 291.26 ± 0.57 pg/mL, BUN: 23.79 ± 0.14 mg/dL, and Cr: 1.34 ± 0.04 mg/dL) in diabetic rats. Oxidative stress markers of pancreas (MDA: 3.65 ± 0.17 nM TBARS /mg protein, SOD: 3.14 ± 0.28 U/mg protein, CAT: 7.88 ± 0.23 U/mg protein, GSH: 12.63 ± 0.28 µM/g of tissue) were restored to normal as evidenced by histological architecture of pancreatic islet cells. The increased level of pro-inflammatory cytokines and oxidative DNA damage were significantly restored (TNF-α: 54.48 ± 3.19 pg/mL, CRP: 440.22 ± 7.86 ng/mL, and 8-OHdG: 63.65 ± 1.84 ng/mL) by HAHZB in diabetic rats. CONCLUSION: The present findings confirm that the presence of bioactive compounds in HAHZB exert therapeutic protective effect by decreasing oxidative, inflammation and pancreatic β-cell damage in oxidative stress induced diabetic rats. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/32535241/The_modulatory_role_of_prime_identified_compounds_in_the_bioactive_fraction_of_Homalium_zeylanicum_in_high_fat_diet_fed_streptozotocin_induced_type_2_diabetic_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(20)31958-9 DB - PRIME DP - Unbound Medicine ER -