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Novel Biosynthesis of Copper Nanoparticles Using Zingiber and Allium sp. with Synergic Effect of Doxycycline for Anticancer and Bactericidal Activity.
Curr Microbiol. 2020 Sep; 77(9):2287-2299.CM

Abstract

Copper nanoparticles (CuNPs), due to their cost-effective synthesis, interesting properties, and a wide range of applications in conductive inks, cooling fluids, biomedical field, and catalysis, have attracted the attention of scientific community in recent years. The aim of the present study was to develop and characterize antibacterial and anticancer CuNPs synthesized via chemical and biological methods, and further synthesize CuNPs conjugated with doxycycline to study their synergic effect. During the chemical synthesis, ascorbic acid was used as a stabilizing agent, while Zingiber officinale and Allium sativum-derived extracts were used during the biological methods for synthesis of CuNPs. Characterization of CuNPs was performed by transmission electron microscopy (TEM), UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), and X-ray crystallography (XRD). Antimicrobial evaluation of the nanomaterials against Pseudomonas aeruginosa and Escherichia coli was performed by using disk diffusion method, while anticancer behavior against HeLa and HepG2 cell lines was studied by MTT assay. TEM revealed spherical-shaped nanoparticles with mean size of 22.70 ± 5.67, 35.01 ± 5.84, and 19.02 ± 2.41 nm for CuNPs, Gin-CuNPs, and Gar-CuNPs, respectively, and surface plasmon resonance peaks were obtained at 570 nm, 575 nm, and 610 nm for CuNPs, Gar-CuNPs, and Gin-CuNPs, respectively. The results of FTIR confirmed the consumption of biomolecules from the plant extracts for the synthesis of CuNPs. XRD analysis also confirmed synthesis of CuNPs. Doxycycline-conjugated NPs exhibited more antibacterial effects than doxycycline or CuNPs alone. Copper nanoparticles prepared by biological synthesis are cost-effective and eco-friendly as compared to their chemical counterparts. The chemically synthesized nanoparticles displayed more significant antimicrobial activity when capped with doxycycline than Z. officinale and A. sativum-mediated CuNPs; however, green-synthesized nanoparticles showed greater anticancer activity than their chemical counterparts.

Authors+Show Affiliations

Department of Zoology, Government College University, Lahore, 54000, Pakistan. atif@gcu.edu.pk.Department of Zoology, Government College University, Lahore, 54000, Pakistan.Department of Zoology, Government College University, Lahore, 54000, Pakistan.Department of Zoology, Government College University, Lahore, 54000, Pakistan.Department of Zoology, Government College University, Lahore, 54000, Pakistan.Department of Chemistry, Government College University, Lahore, 54000, Pakistan.Department of Chemistry, Government College University, Lahore, 54000, Pakistan.Department of Chemistry, Government College University, Lahore, 54000, Pakistan.Department of Zoology, Government College University, Lahore, 54000, Pakistan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32535649

Citation

Yaqub, Atif, et al. "Novel Biosynthesis of Copper Nanoparticles Using Zingiber and Allium Sp. With Synergic Effect of Doxycycline for Anticancer and Bactericidal Activity." Current Microbiology, vol. 77, no. 9, 2020, pp. 2287-2299.
Yaqub A, Malkani N, Shabbir A, et al. Novel Biosynthesis of Copper Nanoparticles Using Zingiber and Allium sp. with Synergic Effect of Doxycycline for Anticancer and Bactericidal Activity. Curr Microbiol. 2020;77(9):2287-2299.
Yaqub, A., Malkani, N., Shabbir, A., Ditta, S. A., Tanvir, F., Ali, S., Naz, M., Kazmi, S. A. R., & Ullah, R. (2020). Novel Biosynthesis of Copper Nanoparticles Using Zingiber and Allium sp. with Synergic Effect of Doxycycline for Anticancer and Bactericidal Activity. Current Microbiology, 77(9), 2287-2299. https://doi.org/10.1007/s00284-020-02058-4
Yaqub A, et al. Novel Biosynthesis of Copper Nanoparticles Using Zingiber and Allium Sp. With Synergic Effect of Doxycycline for Anticancer and Bactericidal Activity. Curr Microbiol. 2020;77(9):2287-2299. PubMed PMID: 32535649.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel Biosynthesis of Copper Nanoparticles Using Zingiber and Allium sp. with Synergic Effect of Doxycycline for Anticancer and Bactericidal Activity. AU - Yaqub,Atif, AU - Malkani,Naila, AU - Shabbir,Arifa, AU - Ditta,Sarwar Allah, AU - Tanvir,Fouzia, AU - Ali,Shaista, AU - Naz,Misbah, AU - Kazmi,Syed Akif Raza, AU - Ullah,Rehan, Y1 - 2020/06/13/ PY - 2019/11/05/received PY - 2020/05/25/accepted PY - 2020/6/15/pubmed PY - 2021/5/15/medline PY - 2020/6/15/entrez SP - 2287 EP - 2299 JF - Current microbiology JO - Curr Microbiol VL - 77 IS - 9 N2 - Copper nanoparticles (CuNPs), due to their cost-effective synthesis, interesting properties, and a wide range of applications in conductive inks, cooling fluids, biomedical field, and catalysis, have attracted the attention of scientific community in recent years. The aim of the present study was to develop and characterize antibacterial and anticancer CuNPs synthesized via chemical and biological methods, and further synthesize CuNPs conjugated with doxycycline to study their synergic effect. During the chemical synthesis, ascorbic acid was used as a stabilizing agent, while Zingiber officinale and Allium sativum-derived extracts were used during the biological methods for synthesis of CuNPs. Characterization of CuNPs was performed by transmission electron microscopy (TEM), UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), and X-ray crystallography (XRD). Antimicrobial evaluation of the nanomaterials against Pseudomonas aeruginosa and Escherichia coli was performed by using disk diffusion method, while anticancer behavior against HeLa and HepG2 cell lines was studied by MTT assay. TEM revealed spherical-shaped nanoparticles with mean size of 22.70 ± 5.67, 35.01 ± 5.84, and 19.02 ± 2.41 nm for CuNPs, Gin-CuNPs, and Gar-CuNPs, respectively, and surface plasmon resonance peaks were obtained at 570 nm, 575 nm, and 610 nm for CuNPs, Gar-CuNPs, and Gin-CuNPs, respectively. The results of FTIR confirmed the consumption of biomolecules from the plant extracts for the synthesis of CuNPs. XRD analysis also confirmed synthesis of CuNPs. Doxycycline-conjugated NPs exhibited more antibacterial effects than doxycycline or CuNPs alone. Copper nanoparticles prepared by biological synthesis are cost-effective and eco-friendly as compared to their chemical counterparts. The chemically synthesized nanoparticles displayed more significant antimicrobial activity when capped with doxycycline than Z. officinale and A. sativum-mediated CuNPs; however, green-synthesized nanoparticles showed greater anticancer activity than their chemical counterparts. SN - 1432-0991 UR - https://www.unboundmedicine.com/medline/citation/32535649/Novel_Biosynthesis_of_Copper_Nanoparticles_Using_Zingiber_and_Allium_sp__with_Synergic_Effect_of_Doxycycline_for_Anticancer_and_Bactericidal_Activity_ L2 - https://dx.doi.org/10.1007/s00284-020-02058-4 DB - PRIME DP - Unbound Medicine ER -