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Hirsutanol A exhibits neuroprotective activities against sevoflurane-induced neurotoxicity in aged rats.
Anat Rec (Hoboken). 2020 Jun 13 [Online ahead of print]AR

Abstract

The neurotoxicity of the inhaled anesthetic, sevoflurane, has been documented in a number of studies. In this study, we conducted experiments to investigate whether Hirsutanol A (HA), a sesquiterpene compound from the fungus, Chondrostereum sp., can provide protection from sevoflurane-induced neurological toxicity in aged rats, and analyzed the underlying mechanisms. The cognitive dysfunction of rats following sevoflurane exposure was evaluated by behavioral tests. The neuronal cell survival was determined by Nissl staining. In addition, human neuroblastoma H4 cells were exposed to sevoflurane to establish an in vitro model. Apoptotic marker expression in hippocampal tissue was determined by western blotting. Cell apoptosis in vitro was also examined by TUNEL assay and flow cytometry. The expression and translocation of Nrf2 were examined by both western blot and immunofluorescence staining. Our results show that HA significantly attenuated sevoflurane-induced cognitive impairment in aged rats. In addition, HA treatment decreased sevoflurane-induced neuronal apoptosis in the hippocampus and alleviated Aβ accumulation. Our results also show that the neuroprotective effect of HA is associated with the activation of Nrf2 signaling. Human neuroblastoma H4 cells were used as a model to examine the protective activity of HA against sevoflurane-induced neurotoxicity. In addition, our results show that the inhibition of Nrf2 by a specific inhibitor or targeting siRNA significantly compromises the attenuating effect of HA on sevoflurane-induced cell apoptosis and Aβ accumulation. Our results suggest that HA may function as a neuroprotective agent against sevoflurane-induced neurotoxicity.

Authors+Show Affiliations

Department of Anesthesiology, Xi'an High Tech Hospital, Xi'an, Shaanxi, China.Department of Anesthesiology, Xi'an High Tech Hospital, Xi'an, Shaanxi, China.Department of Anesthesiology, Xi'an No.1 Hospital, Xi'an, Shaanxi, China.Department of Anesthesiology, Xi'an High Tech Hospital, Xi'an, Shaanxi, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32536020

Citation

Zhou, Hong-She, et al. "Hirsutanol a Exhibits Neuroprotective Activities Against Sevoflurane-induced Neurotoxicity in Aged Rats." Anatomical Record (Hoboken, N.J. : 2007), 2020.
Zhou HS, Cao SM, Liao HS, et al. Hirsutanol A exhibits neuroprotective activities against sevoflurane-induced neurotoxicity in aged rats. Anat Rec (Hoboken). 2020.
Zhou, H. S., Cao, S. M., Liao, H. S., & Huo, H. Y. (2020). Hirsutanol A exhibits neuroprotective activities against sevoflurane-induced neurotoxicity in aged rats. Anatomical Record (Hoboken, N.J. : 2007). https://doi.org/10.1002/ar.24473
Zhou HS, et al. Hirsutanol a Exhibits Neuroprotective Activities Against Sevoflurane-induced Neurotoxicity in Aged Rats. Anat Rec (Hoboken). 2020 Jun 13; PubMed PMID: 32536020.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hirsutanol A exhibits neuroprotective activities against sevoflurane-induced neurotoxicity in aged rats. AU - Zhou,Hong-She, AU - Cao,Shu-Mei, AU - Liao,Hua-Shan, AU - Huo,Hong-Yan, Y1 - 2020/06/13/ PY - 2019/12/10/received PY - 2020/03/26/revised PY - 2020/04/03/accepted PY - 2020/6/15/pubmed PY - 2020/6/15/medline PY - 2020/6/15/entrez KW - Hirsutanol A KW - Nrf2 KW - aged rats KW - sevoflurane JF - Anatomical record (Hoboken, N.J. : 2007) JO - Anat Rec (Hoboken) N2 - The neurotoxicity of the inhaled anesthetic, sevoflurane, has been documented in a number of studies. In this study, we conducted experiments to investigate whether Hirsutanol A (HA), a sesquiterpene compound from the fungus, Chondrostereum sp., can provide protection from sevoflurane-induced neurological toxicity in aged rats, and analyzed the underlying mechanisms. The cognitive dysfunction of rats following sevoflurane exposure was evaluated by behavioral tests. The neuronal cell survival was determined by Nissl staining. In addition, human neuroblastoma H4 cells were exposed to sevoflurane to establish an in vitro model. Apoptotic marker expression in hippocampal tissue was determined by western blotting. Cell apoptosis in vitro was also examined by TUNEL assay and flow cytometry. The expression and translocation of Nrf2 were examined by both western blot and immunofluorescence staining. Our results show that HA significantly attenuated sevoflurane-induced cognitive impairment in aged rats. In addition, HA treatment decreased sevoflurane-induced neuronal apoptosis in the hippocampus and alleviated Aβ accumulation. Our results also show that the neuroprotective effect of HA is associated with the activation of Nrf2 signaling. Human neuroblastoma H4 cells were used as a model to examine the protective activity of HA against sevoflurane-induced neurotoxicity. In addition, our results show that the inhibition of Nrf2 by a specific inhibitor or targeting siRNA significantly compromises the attenuating effect of HA on sevoflurane-induced cell apoptosis and Aβ accumulation. Our results suggest that HA may function as a neuroprotective agent against sevoflurane-induced neurotoxicity. SN - 1932-8494 UR - https://www.unboundmedicine.com/medline/citation/32536020/Hirsutanol_A_exhibits_neuroprotective_activities_against_sevoflurane_induced_neurotoxicity_in_aged_rats_ L2 - https://doi.org/10.1002/ar.24473 DB - PRIME DP - Unbound Medicine ER -
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