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Revealing the Venomous Secrets of the Spider's Web.
J Proteome Res. 2020 Jun 30 [Online ahead of print]JP

Abstract

Orb-weaving spiders use a highly strong, sticky and elastic web to catch their prey. These web properties alone would be enough for the entrapment of prey; however, these spiders may be hiding venomous secrets in the web, which current research is revealing. Here, we provide strong proteotranscriptomic evidence for the presence of toxin/neurotoxin-like proteins, defensins, and proteolytic enzymes on the web silk from Nephila clavipes spider. The results from quantitative-based transcriptomic and proteomic approaches showed that silk-producing glands produce an extensive repertoire of toxin/neurotoxin-like proteins, similar to those already reported in spider venoms. Meanwhile, the insect toxicity results demonstrated that these toxic components can be lethal and/or paralytic chemical weapons used for prey capture on the web, and the presence of fatty acids in the web may be a responsible mechanism opening the way to the web toxins for accessing the interior of prey's body, as shown here. Comparative phylogenomic-level evolutionary analyses revealed orthologous genes among two spider groups, Araneomorphae and Mygalomorphae, and the findings showed protein sequences similar to toxins found in the taxa Scorpiones and Hymenoptera in addition to Araneae. Overall, these data represent a valuable resource to further investigate other spider web toxin systems and also suggest that N. clavipes web is not a passive mechanical trap for prey capture, but it exerts an active role in prey paralysis/killing using a series of neurotoxins.

Authors+Show Affiliations

Center of the Study of Social Insects, Department of General and Applied Biology, Institute of Biosciences of Rio Claro, University of São Paulo State (UNESP), Rio Claro, SP 13506-900, Brazil.Center of the Study of Social Insects, Department of General and Applied Biology, Institute of Biosciences of Rio Claro, University of São Paulo State (UNESP), Rio Claro, SP 13506-900, Brazil.Center of the Study of Social Insects, Department of General and Applied Biology, Institute of Biosciences of Rio Claro, University of São Paulo State (UNESP), Rio Claro, SP 13506-900, Brazil.Department of Pharmaceutical Chemistry, University of Vienna, Vienna 1090, Austria.Department of Pharmaceutical Chemistry, University of Vienna, Vienna 1090, Austria.Division of Computational System Biology, Department of Microbiology and Ecosystem Science, University of Vienna, 1090 Vienna, Austria.Division of Computational System Biology, Department of Microbiology and Ecosystem Science, University of Vienna, 1090 Vienna, Austria.Division of Computational System Biology, Department of Microbiology and Ecosystem Science, University of Vienna, 1090 Vienna, Austria.Department of Laboratory Medicine and Core Facility Genomics, Medical University of Vienna, 1090 Vienna, Austria.Center of the Study of Social Insects, Department of General and Applied Biology, Institute of Biosciences of Rio Claro, University of São Paulo State (UNESP), Rio Claro, SP 13506-900, Brazil.Paracelsus Medical University, A 5020 Salzburg, Austria.Center of the Study of Social Insects, Department of General and Applied Biology, Institute of Biosciences of Rio Claro, University of São Paulo State (UNESP), Rio Claro, SP 13506-900, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32538095

Citation

Esteves, Franciele Grego, et al. "Revealing the Venomous Secrets of the Spider's Web." Journal of Proteome Research, 2020.
Esteves FG, Dos Santos-Pinto JRA, Ferro M, et al. Revealing the Venomous Secrets of the Spider's Web. J Proteome Res. 2020.
Esteves, F. G., Dos Santos-Pinto, J. R. A., Ferro, M., Sialana, F. J., Smidak, R., Rares, L. C., Nussbaumer, T., Rattei, T., Bilban, M., Bacci Júnior, M., Lubec, G., & Palma, M. S. (2020). Revealing the Venomous Secrets of the Spider's Web. Journal of Proteome Research. https://doi.org/10.1021/acs.jproteome.0c00086
Esteves FG, et al. Revealing the Venomous Secrets of the Spider's Web. J Proteome Res. 2020 Jun 30; PubMed PMID: 32538095.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Revealing the Venomous Secrets of the Spider's Web. AU - Esteves,Franciele Grego, AU - Dos Santos-Pinto,José Roberto Aparecido, AU - Ferro,Milene, AU - Sialana,Fernando J, AU - Smidak,Roman, AU - Rares,Lucaciu Calin, AU - Nussbaumer,Thomas, AU - Rattei,Thomas, AU - Bilban,Martin, AU - Bacci Júnior,Mauricio, AU - Lubec,Gert, AU - Palma,Mario Sergio, Y1 - 2020/06/30/ PY - 2020/6/17/pubmed PY - 2020/6/17/medline PY - 2020/6/16/entrez KW - LCMS-based proteomics KW - de novo transcriptome assembly KW - neurotoxins KW - spider silk-producing glands KW - web silk toxins JF - Journal of proteome research JO - J. Proteome Res. N2 - Orb-weaving spiders use a highly strong, sticky and elastic web to catch their prey. These web properties alone would be enough for the entrapment of prey; however, these spiders may be hiding venomous secrets in the web, which current research is revealing. Here, we provide strong proteotranscriptomic evidence for the presence of toxin/neurotoxin-like proteins, defensins, and proteolytic enzymes on the web silk from Nephila clavipes spider. The results from quantitative-based transcriptomic and proteomic approaches showed that silk-producing glands produce an extensive repertoire of toxin/neurotoxin-like proteins, similar to those already reported in spider venoms. Meanwhile, the insect toxicity results demonstrated that these toxic components can be lethal and/or paralytic chemical weapons used for prey capture on the web, and the presence of fatty acids in the web may be a responsible mechanism opening the way to the web toxins for accessing the interior of prey's body, as shown here. Comparative phylogenomic-level evolutionary analyses revealed orthologous genes among two spider groups, Araneomorphae and Mygalomorphae, and the findings showed protein sequences similar to toxins found in the taxa Scorpiones and Hymenoptera in addition to Araneae. Overall, these data represent a valuable resource to further investigate other spider web toxin systems and also suggest that N. clavipes web is not a passive mechanical trap for prey capture, but it exerts an active role in prey paralysis/killing using a series of neurotoxins. SN - 1535-3907 UR - https://www.unboundmedicine.com/medline/citation/32538095/Revealing_the_venomous_secrets_of_the_spider's_web L2 - https://doi.org/10.1021/acs.jproteome.0c00086 DB - PRIME DP - Unbound Medicine ER -
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