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Tetramethylpyrazine production from edible materials by the probiotic Bacillus coagulans.
Prep Biochem Biotechnol. 2020 Jun 13 [Online ahead of print]PB

Abstract

2,3,5,6-Tetramethylpyrazine (TMP) has health care functions, especially for cardiovascular and cerebrovascular health. In this study, we found that Bacillus coagulans, a well-known probiotic, has the capability to produce acetoin, a precursor of TMP. The culture conditions and medium for the production of TMP by B. coagulans CICC 20138 were optimized. Then, a novel three-step process was successfully performed for the production of TMP from edible materials by B. coagulans. First, in the acetoin enrichment process, 12.61 ± 0.34 g/L acetoin was generated at 36 h. Second, in the spore enrichment process, various factors were optimized to make the bacteria produce more spores to improve the resistance to subsequent high-temperature reactions. Third, in the TMP enrichment process, the final concentration of TMP and B. coagulans spores contained in the product reached 2.54 ± 0.26 g/L and 8.81 × 108 CFU/mL at 46 h, respectively. This is the first report of using a probiotic bacterium to produce TMP. Using edible materials and the probiotic strain, this work provides a novel method for the production of a TMP food additive rich in B. coagulans spores.

Authors+Show Affiliations

Center for Bioengineering & Biotechnology and State Key Laboratory of Heavy Oil Processing, China University of Petroleum (East China), Qingdao, China.Center for Bioengineering & Biotechnology and State Key Laboratory of Heavy Oil Processing, China University of Petroleum (East China), Qingdao, China.Center for Bioengineering & Biotechnology and State Key Laboratory of Heavy Oil Processing, China University of Petroleum (East China), Qingdao, China.Center for Bioengineering & Biotechnology and State Key Laboratory of Heavy Oil Processing, China University of Petroleum (East China), Qingdao, China.Center for Bioengineering & Biotechnology and State Key Laboratory of Heavy Oil Processing, China University of Petroleum (East China), Qingdao, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32538266

Citation

Zhong, Haoxuan, et al. "Tetramethylpyrazine Production From Edible Materials By the Probiotic Bacillus Coagulans." Preparative Biochemistry & Biotechnology, 2020, pp. 1-8.
Zhong H, Shen J, Meng Z, et al. Tetramethylpyrazine production from edible materials by the probiotic Bacillus coagulans. Prep Biochem Biotechnol. 2020.
Zhong, H., Shen, J., Meng, Z., Zhao, J. Y., & Xiao, Z. (2020). Tetramethylpyrazine production from edible materials by the probiotic Bacillus coagulans. Preparative Biochemistry & Biotechnology, 1-8. https://doi.org/10.1080/10826068.2020.1774777
Zhong H, et al. Tetramethylpyrazine Production From Edible Materials By the Probiotic Bacillus Coagulans. Prep Biochem Biotechnol. 2020 Jun 13;1-8. PubMed PMID: 32538266.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tetramethylpyrazine production from edible materials by the probiotic Bacillus coagulans. AU - Zhong,Haoxuan, AU - Shen,Jie, AU - Meng,Zhe, AU - Zhao,Jing-Yi, AU - Xiao,Zijun, Y1 - 2020/06/13/ PY - 2020/6/16/entrez KW - Acetoin KW - Bacillus coagulans KW - probiotics KW - tetramethylpyrazine SP - 1 EP - 8 JF - Preparative biochemistry & biotechnology JO - Prep. Biochem. Biotechnol. N2 - 2,3,5,6-Tetramethylpyrazine (TMP) has health care functions, especially for cardiovascular and cerebrovascular health. In this study, we found that Bacillus coagulans, a well-known probiotic, has the capability to produce acetoin, a precursor of TMP. The culture conditions and medium for the production of TMP by B. coagulans CICC 20138 were optimized. Then, a novel three-step process was successfully performed for the production of TMP from edible materials by B. coagulans. First, in the acetoin enrichment process, 12.61 ± 0.34 g/L acetoin was generated at 36 h. Second, in the spore enrichment process, various factors were optimized to make the bacteria produce more spores to improve the resistance to subsequent high-temperature reactions. Third, in the TMP enrichment process, the final concentration of TMP and B. coagulans spores contained in the product reached 2.54 ± 0.26 g/L and 8.81 × 108 CFU/mL at 46 h, respectively. This is the first report of using a probiotic bacterium to produce TMP. Using edible materials and the probiotic strain, this work provides a novel method for the production of a TMP food additive rich in B. coagulans spores. SN - 1532-2297 UR - https://www.unboundmedicine.com/medline/citation/32538266/Tetramethylpyrazine_production_from_edible_materials_by_the_probiotic_Bacillus_coagulans L2 - http://www.tandfonline.com/doi/full/10.1080/10826068.2020.1774777 DB - PRIME DP - Unbound Medicine ER -
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