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Dietary β-hydroxy-β-methylbutyrate improves intestinal function in weaned piglets after lipopolysaccharide challenge.
Nutrition. 2020 Apr 21; 78:110839.N

Abstract

OBJECTIVES

The aim of this study was to explore the effects of β-hydroxy-β-methylbutyrate (HMB) on intestinal function of lipopolysaccharide (LPS)-challenged piglets.

METHODS

Forty weaned piglets were used in a 2 × 2 factorial design. The major factors were challenge (saline or LPS) and diet (basal diet or 0.6% HMB-Ca diet). After 15 d of treatment with LPS or HMB, blood and intestine samples were obtained.

RESULTS

The results showed that in LPS-injected pigs, HMB supplementation significantly increased jejunal villus height and ileal villus height-to-crypt depth ratio and decreased ileal crypt depth (P < 0.05). HMB also improved intestinal function indicated by elevated activities of intestinal mucosal disaccharidase and tricarboxylic acid cycle key enzymes. Furthermore, HMB significantly downregulated mRNA expression of Sirt1 in jejunum and mRNA expression of AMPKα1 and Sirt1 in ileum (P < 0.05), with a concurrent decrease of AMPKα phosphorylation in jejunum and ileum. Microbiota analysis indicated that HMB supplementation significantly increased α-diversity and affected relative abundances of Romboutsia and Sarcina at the genus level, accompanied by increased concentrations of all short-chain fatty acids except propionate in the terminate ileum of LPS-injected piglets.

CONCLUSION

Dietary HMB supplementation could improve intestinal integrity, function, microbiota communities, and short-chain fatty acid concentrations in LPS-challenged piglets, suggesting its potential usage as a feed additive in weaned piglets to alleviate intestinal dysfunction triggered by immune stress.

Authors+Show Affiliations

Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China; Guangdong Provincial Key Laboratory of Animal Nutrition Regulation, South China Agricultural University, Guangzhou, Guangdong, China.Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China; Guangdong Provincial Key Laboratory of Animal Nutrition Regulation, South China Agricultural University, Guangzhou, Guangdong, China.Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China. Electronic address: duanyehui@isa.ac.cn.Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China; Guangdong Provincial Key Laboratory of Animal Nutrition Regulation, South China Agricultural University, Guangzhou, Guangdong, China.Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China.Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China.Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, China; Hunan Co-Innovation Center of Animal Production Safety, CICAPS; Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Changsha, Hunan, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32540677

Citation

Zheng, Changbing, et al. "Dietary Β-hydroxy-β-methylbutyrate Improves Intestinal Function in Weaned Piglets After Lipopolysaccharide Challenge." Nutrition (Burbank, Los Angeles County, Calif.), vol. 78, 2020, p. 110839.
Zheng C, Song B, Duan Y, et al. Dietary β-hydroxy-β-methylbutyrate improves intestinal function in weaned piglets after lipopolysaccharide challenge. Nutrition. 2020;78:110839.
Zheng, C., Song, B., Duan, Y., Zhong, Y., Yan, Z., Zhang, S., & Li, F. (2020). Dietary β-hydroxy-β-methylbutyrate improves intestinal function in weaned piglets after lipopolysaccharide challenge. Nutrition (Burbank, Los Angeles County, Calif.), 78, 110839. https://doi.org/10.1016/j.nut.2020.110839
Zheng C, et al. Dietary Β-hydroxy-β-methylbutyrate Improves Intestinal Function in Weaned Piglets After Lipopolysaccharide Challenge. Nutrition. 2020 Apr 21;78:110839. PubMed PMID: 32540677.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary β-hydroxy-β-methylbutyrate improves intestinal function in weaned piglets after lipopolysaccharide challenge. AU - Zheng,Changbing, AU - Song,Bo, AU - Duan,Yehui, AU - Zhong,Yinzhao, AU - Yan,Zhaoming, AU - Zhang,Shiyu, AU - Li,Fengna, Y1 - 2020/04/21/ PY - 2019/08/11/received PY - 2020/03/15/revised PY - 2020/04/03/accepted PY - 2020/6/17/pubmed PY - 2020/6/17/medline PY - 2020/6/17/entrez KW - Intestinal function KW - Lipopolysaccharide KW - Piglets KW - β-hydroxy-β-methylbutyrate SP - 110839 EP - 110839 JF - Nutrition (Burbank, Los Angeles County, Calif.) JO - Nutrition VL - 78 N2 - OBJECTIVES: The aim of this study was to explore the effects of β-hydroxy-β-methylbutyrate (HMB) on intestinal function of lipopolysaccharide (LPS)-challenged piglets. METHODS: Forty weaned piglets were used in a 2 × 2 factorial design. The major factors were challenge (saline or LPS) and diet (basal diet or 0.6% HMB-Ca diet). After 15 d of treatment with LPS or HMB, blood and intestine samples were obtained. RESULTS: The results showed that in LPS-injected pigs, HMB supplementation significantly increased jejunal villus height and ileal villus height-to-crypt depth ratio and decreased ileal crypt depth (P < 0.05). HMB also improved intestinal function indicated by elevated activities of intestinal mucosal disaccharidase and tricarboxylic acid cycle key enzymes. Furthermore, HMB significantly downregulated mRNA expression of Sirt1 in jejunum and mRNA expression of AMPKα1 and Sirt1 in ileum (P < 0.05), with a concurrent decrease of AMPKα phosphorylation in jejunum and ileum. Microbiota analysis indicated that HMB supplementation significantly increased α-diversity and affected relative abundances of Romboutsia and Sarcina at the genus level, accompanied by increased concentrations of all short-chain fatty acids except propionate in the terminate ileum of LPS-injected piglets. CONCLUSION: Dietary HMB supplementation could improve intestinal integrity, function, microbiota communities, and short-chain fatty acid concentrations in LPS-challenged piglets, suggesting its potential usage as a feed additive in weaned piglets to alleviate intestinal dysfunction triggered by immune stress. SN - 1873-1244 UR - https://www.unboundmedicine.com/medline/citation/32540677/Dietary_β_hydroxy_β_methylbutyrate_improves_intestinal_function_in_weaned_piglets_after_lipopolysaccharide_challenge_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0899-9007(20)30122-2 DB - PRIME DP - Unbound Medicine ER -
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