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High three pro-nuclei (3PN) zygotes proportion associated with normal embryo multinucleation at the two-cell stage: two cases report.
Gynecol Endocrinol. 2020 Jun 16 [Online ahead of print]GE

Abstract

Objective:

Blastomere multinucleation at the two-cell stage (MN2) is a common nuclear abnormality observed in early human embryos and known to decrease the implantation rate. The aim of this study is to explore whether or not there is a link between high 3PN zygotes proportion and MN2 incidence.

Methods:

For embryo culture in the conventional incubator, the evaluation of nuclear status of two-cell stage is usually not performed. Therefore, the MN2 phenomenon is easily ignored. The time-lapse monitoring system (TLS) offers a promising new method to evaluate embryo development. We reported two cases who had single 2PN zygote and more than four 3PN zygotes in the conventional in-vitro fertilization (c-IVF) cycle.

Results:

We observed the MN2 incidence in the single normal embryo by TLS which suggested that it might be resulted from high 3PN zygotes proportion incidence. No available embryo was obtained in the first c-IVF cycle and the intracytoplasmic sperm injection (ICSI) treatment was performed in the second cycle. In subsequent ICSI cycles, we observed no 3PN zygotes incidence and transferred two day 3 embryos for the patients. Finally, the two couples successfully obtained healthy babies.

Conclusions:

High 3PN zygotes proportion might be associated with the MN2 incidence.

Authors+Show Affiliations

The ART Center, Northwest Women and Children's Hospital, Xi'an, China.The ART Center, Northwest Women and Children's Hospital, Xi'an, China.The ART Center, Northwest Women and Children's Hospital, Xi'an, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32544013

Citation

Li, Mingzhao, et al. "High Three Pro-nuclei (3PN) Zygotes Proportion Associated With Normal Embryo Multinucleation at the Two-cell Stage: Two Cases Report." Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology, 2020, pp. 1-3.
Li M, Xue X, Shi J. High three pro-nuclei (3PN) zygotes proportion associated with normal embryo multinucleation at the two-cell stage: two cases report. Gynecol Endocrinol. 2020.
Li, M., Xue, X., & Shi, J. (2020). High three pro-nuclei (3PN) zygotes proportion associated with normal embryo multinucleation at the two-cell stage: two cases report. Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology, 1-3. https://doi.org/10.1080/09513590.2020.1779690
Li M, Xue X, Shi J. High Three Pro-nuclei (3PN) Zygotes Proportion Associated With Normal Embryo Multinucleation at the Two-cell Stage: Two Cases Report. Gynecol Endocrinol. 2020 Jun 16;1-3. PubMed PMID: 32544013.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High three pro-nuclei (3PN) zygotes proportion associated with normal embryo multinucleation at the two-cell stage: two cases report. AU - Li,Mingzhao, AU - Xue,Xia, AU - Shi,Juanzi, Y1 - 2020/06/16/ PY - 2020/6/17/entrez PY - 2020/6/17/pubmed PY - 2020/6/17/medline KW - Blastomere multinucleation KW - three pro-nuclei KW - time-lapse SP - 1 EP - 3 JF - Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology JO - Gynecol. Endocrinol. N2 - Objective: Blastomere multinucleation at the two-cell stage (MN2) is a common nuclear abnormality observed in early human embryos and known to decrease the implantation rate. The aim of this study is to explore whether or not there is a link between high 3PN zygotes proportion and MN2 incidence.Methods: For embryo culture in the conventional incubator, the evaluation of nuclear status of two-cell stage is usually not performed. Therefore, the MN2 phenomenon is easily ignored. The time-lapse monitoring system (TLS) offers a promising new method to evaluate embryo development. We reported two cases who had single 2PN zygote and more than four 3PN zygotes in the conventional in-vitro fertilization (c-IVF) cycle.Results: We observed the MN2 incidence in the single normal embryo by TLS which suggested that it might be resulted from high 3PN zygotes proportion incidence. No available embryo was obtained in the first c-IVF cycle and the intracytoplasmic sperm injection (ICSI) treatment was performed in the second cycle. In subsequent ICSI cycles, we observed no 3PN zygotes incidence and transferred two day 3 embryos for the patients. Finally, the two couples successfully obtained healthy babies.Conclusions: High 3PN zygotes proportion might be associated with the MN2 incidence. SN - 1473-0766 UR - https://www.unboundmedicine.com/medline/citation/32544013/High_three_pro-nuclei_(3PN)_zygotes_proportion_associated_with_normal_embryo_multinucleation_at_the_two-cell_stage:_two_cases_report L2 - http://www.tandfonline.com/doi/full/10.1080/09513590.2020.1779690 DB - PRIME DP - Unbound Medicine ER -
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