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Genome Wild Analysis and Molecular Understanding of the Aquaporin Diversity in Olive Trees (Olea Europaea L.).
Int J Mol Sci. 2020 Jun 11; 21(11)IJ

Abstract

Cellular aquaporin water channels (AQPs) constitute a large family of transmembrane proteins present throughout all kingdoms of life, playing important roles in the uptake of water and many solutes across the membranes. In olive trees, AQP diversity, protein features and their biological functions are still largely unknown. This study focuses on the structure and functional and evolution diversity of AQP subfamilies in two olive trees, the wild species Olea europaea var. sylvestris (OeuAQPs) and the domesticated species Olea europaea cv. Picual (OleurAQPs), and describes their involvement in different physiological processes of early plantlet development and in biotic and abiotic stress tolerance in the domesticated species. A scan of genomes from the wild and domesticated olive species revealed the presence of 52 and 79 genes encoding full-length AQP sequences, respectively. Cross-genera phylogenetic analysis with orthologous clustered OleaAQPs into five established subfamilies: PIP, TIP, NIP, SIP, and XIP. Subsequently, gene structures, protein motifs, substrate specificities and cellular localizations of the full length OleaAQPs were predicted. Functional prediction based on the NPA motif, ar/R selectivity filter, Froger's and specificity-determining positions suggested differences in substrate specificities of Olea AQPs. Expression analysis of the OleurAQP genes indicates that some genes are tissue-specific, whereas few others show differential expressions at different developmental stages and in response to various biotic and abiotic stresses. The current study presents the first detailed genome-wide analysis of the AQP gene family in olive trees and it provides valuable information for further functional analysis to infer the role of AQP in the adaptation of olive trees in diverse environmental conditions in order to help the genetic improvement of domesticated olive trees.

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Authors+Show Affiliations

Faize M
Laboratory of Plant Biotechnology, Ecology and Ecosystem Valorization, Faculty of Sciences, University Chouaib Doukkali, El Jadida 24000, Morocco.
Fumanal B
Université Clermont Auvergne, INRAE, PIAF, 63000 Clermont-Ferrand, France.
Luque F
Department of Experimental Biology, Center for Advanced Studies in Olive Grove and Olive Oils, University of Jaén, 23071 Jaén, Spain.
Ramírez-Tejero JA
Department of Experimental Biology, Center for Advanced Studies in Olive Grove and Olive Oils, University of Jaén, 23071 Jaén, Spain.
Zou Z
Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China.
Qiao X
Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China.
Faize L
Group of Fruit Tree Biotechnology, Department of Plant Breeding, Murcia University, CEBAS CSIC, 30100 Murcia, Spain.
Gousset-Dupont A
Université Clermont Auvergne, INRAE, PIAF, 63000 Clermont-Ferrand, France.
Roeckel-Drevet P
Université Clermont Auvergne, INRAE, PIAF, 63000 Clermont-Ferrand, France.
Label P
Université Clermont Auvergne, INRAE, PIAF, 63000 Clermont-Ferrand, France.
Venisse JS
Université Clermont Auvergne, INRAE, PIAF, 63000 Clermont-Ferrand, France.

MeSH

Amino Acid MotifsAquaporinsAscomycotaDomesticationGene Expression Regulation, PlantGenetic VariationGenome-Wide Association StudyMultigene FamilyOleaPhylogenyPlant ProteinsSeedlingsStress, PhysiologicalTrees

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32545387

Citation

Faize, Mohamed, et al. "Genome Wild Analysis and Molecular Understanding of the Aquaporin Diversity in Olive Trees (Olea Europaea L.)." International Journal of Molecular Sciences, vol. 21, no. 11, 2020.
Faize M, Fumanal B, Luque F, et al. Genome Wild Analysis and Molecular Understanding of the Aquaporin Diversity in Olive Trees (Olea Europaea L.). Int J Mol Sci. 2020;21(11).
Faize, M., Fumanal, B., Luque, F., Ramírez-Tejero, J. A., Zou, Z., Qiao, X., Faize, L., Gousset-Dupont, A., Roeckel-Drevet, P., Label, P., & Venisse, J. S. (2020). Genome Wild Analysis and Molecular Understanding of the Aquaporin Diversity in Olive Trees (Olea Europaea L.). International Journal of Molecular Sciences, 21(11). https://doi.org/10.3390/ijms21114183
Faize M, et al. Genome Wild Analysis and Molecular Understanding of the Aquaporin Diversity in Olive Trees (Olea Europaea L.). Int J Mol Sci. 2020 Jun 11;21(11) PubMed PMID: 32545387.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome Wild Analysis and Molecular Understanding of the Aquaporin Diversity in Olive Trees (Olea Europaea L.). AU - Faize,Mohamed, AU - Fumanal,Boris, AU - Luque,Francisco, AU - Ramírez-Tejero,Jorge A, AU - Zou,Zhi, AU - Qiao,Xueying, AU - Faize,Lydia, AU - Gousset-Dupont,Aurélie, AU - Roeckel-Drevet,Patricia, AU - Label,Philippe, AU - Venisse,Jean-Stéphane, Y1 - 2020/06/11/ PY - 2020/05/16/received PY - 2020/06/02/revised PY - 2020/06/03/accepted PY - 2020/6/18/entrez PY - 2020/6/18/pubmed PY - 2021/3/26/medline KW - Olea europaea KW - Oleaceae evolution KW - RNA-seq KW - Verticillium dahliae KW - aquaporin KW - cold and wound stress KW - functional diversity KW - plant domestication JF - International journal of molecular sciences JO - Int J Mol Sci VL - 21 IS - 11 N2 - Cellular aquaporin water channels (AQPs) constitute a large family of transmembrane proteins present throughout all kingdoms of life, playing important roles in the uptake of water and many solutes across the membranes. In olive trees, AQP diversity, protein features and their biological functions are still largely unknown. This study focuses on the structure and functional and evolution diversity of AQP subfamilies in two olive trees, the wild species Olea europaea var. sylvestris (OeuAQPs) and the domesticated species Olea europaea cv. Picual (OleurAQPs), and describes their involvement in different physiological processes of early plantlet development and in biotic and abiotic stress tolerance in the domesticated species. A scan of genomes from the wild and domesticated olive species revealed the presence of 52 and 79 genes encoding full-length AQP sequences, respectively. Cross-genera phylogenetic analysis with orthologous clustered OleaAQPs into five established subfamilies: PIP, TIP, NIP, SIP, and XIP. Subsequently, gene structures, protein motifs, substrate specificities and cellular localizations of the full length OleaAQPs were predicted. Functional prediction based on the NPA motif, ar/R selectivity filter, Froger's and specificity-determining positions suggested differences in substrate specificities of Olea AQPs. Expression analysis of the OleurAQP genes indicates that some genes are tissue-specific, whereas few others show differential expressions at different developmental stages and in response to various biotic and abiotic stresses. The current study presents the first detailed genome-wide analysis of the AQP gene family in olive trees and it provides valuable information for further functional analysis to infer the role of AQP in the adaptation of olive trees in diverse environmental conditions in order to help the genetic improvement of domesticated olive trees. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/32545387/Genome_Wild_Analysis_and_Molecular_Understanding_of_the_Aquaporin_Diversity_in_Olive_Trees__Olea_Europaea_L___ DB - PRIME DP - Unbound Medicine ER -