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A Potent and Safer Anticancer and Antibacterial Taxus-Based Green Synthesized Silver Nanoparticle.
Int J Nanomedicine. 2020; 15:3791-3801.IJ

Abstract

Purpose

Paclitaxel is a generic drug produced based on Taxol which is an extract of Taxus tree, well known for its anticancer and antibacterial effects. This study was aimed at building up an agent with the antibacterial and anticancer benefits of both the silver ions and Taxol, together with less cytotoxic effects.

Materials and Methods

Colloidal silver nanoparticles (AgNPs) were synthesized by reducing aqueous AgNO3 with aqueous Taxus leaf extract at nonphotomediated conditions, without any catalyst, template or surfactant. The AgNP production was confirmed by ultraviolet-visible (UV-VIS) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier-transform infrared (FTI) spectroscopy. The MTT assay for human breast cancer cells as well as the DAPI fluorescent staining microscopy tested the biocompatibility and anticancer effects of AgNPs, silver nitrate, and Taxol. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) techniques were performed to determine the shape and size of the nanoparticles. MTT assay showed the best inhibitory concentration of AgNPs on cancer cells. The antibacterial activity of the three case study materials was tested for gram-positive (Staphylococcus aureus) and gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) using well diffusion test.

Results

This work proposes more anticancer effects for AgNP made by Taxus brevifolia extract, comparing Taxol solution. IC50 was observed as 3.1 mM for Taxol while 1.5 mM for new AgNP. Moreover, Taxus showed no antibacterial effects while the new AgNP showed a dose-dependent biocompatibility along with slightly more antibacterial effects (MIC: 1.6 and 6.6mM for gram-positive and -negative bacteria, respectively) comparing with silver nitrate solution (MIC: 1.5 and 6.2 mM for gram-positive and -negative bacteria, respectively).

Conclusion

The production of herbal-mediated silver nanoparticles may be an efficient substitution for the silver nitrate-based medicines with less side effects.

Authors+Show Affiliations

Department of Chemistry, Arak Islamic Azad University, Arak, Iran.Medical Cellular and Molecular Research Center, School of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran.Department of Microbiology, College of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32547028

Citation

Sarli, Sona, et al. "A Potent and Safer Anticancer and Antibacterial Taxus-Based Green Synthesized Silver Nanoparticle." International Journal of Nanomedicine, vol. 15, 2020, pp. 3791-3801.
Sarli S, Kalani MR, Moradi A. A Potent and Safer Anticancer and Antibacterial Taxus-Based Green Synthesized Silver Nanoparticle. Int J Nanomedicine. 2020;15:3791-3801.
Sarli, S., Kalani, M. R., & Moradi, A. (2020). A Potent and Safer Anticancer and Antibacterial Taxus-Based Green Synthesized Silver Nanoparticle. International Journal of Nanomedicine, 15, 3791-3801. https://doi.org/10.2147/IJN.S251174
Sarli S, Kalani MR, Moradi A. A Potent and Safer Anticancer and Antibacterial Taxus-Based Green Synthesized Silver Nanoparticle. Int J Nanomedicine. 2020;15:3791-3801. PubMed PMID: 32547028.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Potent and Safer Anticancer and Antibacterial Taxus-Based Green Synthesized Silver Nanoparticle. AU - Sarli,Sona, AU - Kalani,Mohamad Reza, AU - Moradi,Abdolvahab, Y1 - 2020/05/28/ PY - 2020/6/18/entrez PY - 2020/6/18/pubmed PY - 2020/7/31/medline KW - Taxus tree extract KW - antibacterial KW - anticancer KW - green synthesis KW - silver nanoparticle SP - 3791 EP - 3801 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 15 N2 - Purpose: Paclitaxel is a generic drug produced based on Taxol which is an extract of Taxus tree, well known for its anticancer and antibacterial effects. This study was aimed at building up an agent with the antibacterial and anticancer benefits of both the silver ions and Taxol, together with less cytotoxic effects. Materials and Methods: Colloidal silver nanoparticles (AgNPs) were synthesized by reducing aqueous AgNO3 with aqueous Taxus leaf extract at nonphotomediated conditions, without any catalyst, template or surfactant. The AgNP production was confirmed by ultraviolet-visible (UV-VIS) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier-transform infrared (FTI) spectroscopy. The MTT assay for human breast cancer cells as well as the DAPI fluorescent staining microscopy tested the biocompatibility and anticancer effects of AgNPs, silver nitrate, and Taxol. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) techniques were performed to determine the shape and size of the nanoparticles. MTT assay showed the best inhibitory concentration of AgNPs on cancer cells. The antibacterial activity of the three case study materials was tested for gram-positive (Staphylococcus aureus) and gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) using well diffusion test. Results: This work proposes more anticancer effects for AgNP made by Taxus brevifolia extract, comparing Taxol solution. IC50 was observed as 3.1 mM for Taxol while 1.5 mM for new AgNP. Moreover, Taxus showed no antibacterial effects while the new AgNP showed a dose-dependent biocompatibility along with slightly more antibacterial effects (MIC: 1.6 and 6.6mM for gram-positive and -negative bacteria, respectively) comparing with silver nitrate solution (MIC: 1.5 and 6.2 mM for gram-positive and -negative bacteria, respectively). Conclusion: The production of herbal-mediated silver nanoparticles may be an efficient substitution for the silver nitrate-based medicines with less side effects. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/32547028/A_Potent_and_Safer_Anticancer_and_Antibacterial_Taxus_Based_Green_Synthesized_Silver_Nanoparticle_ L2 - https://dx.doi.org/10.2147/IJN.S251174 DB - PRIME DP - Unbound Medicine ER -