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Lemon Balm Extract ALS-L1023 Regulates Obesity and Improves Insulin Sensitivity via Activation of Hepatic PPARα in High-Fat Diet-Fed Obese C57BL/6J Mice.
Int J Mol Sci. 2020 Jun 15; 21(12)IJ

Abstract

Our previous studies demonstrated that peroxisome proliferator-activated receptor α (PPARα) activation reduces weight gain and improves insulin sensitivity in obese mice. Since excess lipid accumulation in non-adipose tissues is suggested to be responsible for the development of insulin resistance, this study was undertaken to examine whether the lemon balm extract ALS-L1023 regulates hepatic lipid accumulation, obesity, and insulin resistance and to determine whether its mechanism of action involves PPARα. Administration of ALS-L1023 to high-fat-diet-induced obese mice caused reductions in body weight gain, visceral fat mass, and visceral adipocyte size without changes of food consumption profiles. ALS-L1023 improved hyperglycemia, hyperinsulinemia, glucose and insulin tolerance, and normalized insulin-positive β-cell area in obese mice. ALS-L1023 decreased hepatic lipid accumulation and concomitantly increased the expression of PPARα target genes responsible for fatty acid β-oxidation in livers. In accordance with the in vivo data, ALS-L1023 reduced lipid accumulation and stimulated PPARα reporter gene expression in HepG2 cells. These effects of ALS-L1023 were comparable to those of the PPARα ligand fenofibrate, while the PPARα antagonist GW6471 inhibited the actions of ALS-L1023 on lipid accumulation and PPARα luciferase activity in HepG2 cells. Higher phosphorylated protein kinase B (pAkt)/Akt ratios and lower expression of gluconeogenesis genes were observed in the livers of ALS-L1023-treated mice. These results indicate that ALS-L1023 may inhibit obesity and improve insulin sensitivity in part through inhibition of hepatic lipid accumulation via hepatic PPARα activation.

Authors+Show Affiliations

Department of Biomedical Engineering, Mokwon University, Daejeon 35349, Korea.Department of Biomedical Engineering, Mokwon University, Daejeon 35349, Korea.Department of Microbiology and Immunology, Pusan National University School of Medicine, Busan 50612, Korea.Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.Department of Biomedical Engineering, Mokwon University, Daejeon 35349, Korea.Department of Formula Sciences, College of Oriental Medicine, Dongeui University, Busan 47340, Korea.Department of Biomedical Engineering, Mokwon University, Daejeon 35349, Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32549364

Citation

Lee, Dongju, et al. "Lemon Balm Extract ALS-L1023 Regulates Obesity and Improves Insulin Sensitivity Via Activation of Hepatic PPARα in High-Fat Diet-Fed Obese C57BL/6J Mice." International Journal of Molecular Sciences, vol. 21, no. 12, 2020.
Lee D, Shin Y, Roh JS, et al. Lemon Balm Extract ALS-L1023 Regulates Obesity and Improves Insulin Sensitivity via Activation of Hepatic PPARα in High-Fat Diet-Fed Obese C57BL/6J Mice. Int J Mol Sci. 2020;21(12).
Lee, D., Shin, Y., Roh, J. S., Ahn, J., Jeoong, S., Shin, S. S., & Yoon, M. (2020). Lemon Balm Extract ALS-L1023 Regulates Obesity and Improves Insulin Sensitivity via Activation of Hepatic PPARα in High-Fat Diet-Fed Obese C57BL/6J Mice. International Journal of Molecular Sciences, 21(12). https://doi.org/10.3390/ijms21124256
Lee D, et al. Lemon Balm Extract ALS-L1023 Regulates Obesity and Improves Insulin Sensitivity Via Activation of Hepatic PPARα in High-Fat Diet-Fed Obese C57BL/6J Mice. Int J Mol Sci. 2020 Jun 15;21(12) PubMed PMID: 32549364.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lemon Balm Extract ALS-L1023 Regulates Obesity and Improves Insulin Sensitivity via Activation of Hepatic PPARα in High-Fat Diet-Fed Obese C57BL/6J Mice. AU - Lee,Dongju, AU - Shin,Yujin, AU - Roh,Jong Seong, AU - Ahn,Jiwon, AU - Jeoong,Sunhyo, AU - Shin,Soon Shik, AU - Yoon,Michung, Y1 - 2020/06/15/ PY - 2020/04/20/received PY - 2020/05/24/revised PY - 2020/06/11/accepted PY - 2020/6/19/entrez PY - 2020/6/19/pubmed PY - 2020/6/19/medline KW - PPARα KW - hepatic lipid KW - insulin resistance KW - lemon balm KW - obesity JF - International journal of molecular sciences JO - Int J Mol Sci VL - 21 IS - 12 N2 - Our previous studies demonstrated that peroxisome proliferator-activated receptor α (PPARα) activation reduces weight gain and improves insulin sensitivity in obese mice. Since excess lipid accumulation in non-adipose tissues is suggested to be responsible for the development of insulin resistance, this study was undertaken to examine whether the lemon balm extract ALS-L1023 regulates hepatic lipid accumulation, obesity, and insulin resistance and to determine whether its mechanism of action involves PPARα. Administration of ALS-L1023 to high-fat-diet-induced obese mice caused reductions in body weight gain, visceral fat mass, and visceral adipocyte size without changes of food consumption profiles. ALS-L1023 improved hyperglycemia, hyperinsulinemia, glucose and insulin tolerance, and normalized insulin-positive β-cell area in obese mice. ALS-L1023 decreased hepatic lipid accumulation and concomitantly increased the expression of PPARα target genes responsible for fatty acid β-oxidation in livers. In accordance with the in vivo data, ALS-L1023 reduced lipid accumulation and stimulated PPARα reporter gene expression in HepG2 cells. These effects of ALS-L1023 were comparable to those of the PPARα ligand fenofibrate, while the PPARα antagonist GW6471 inhibited the actions of ALS-L1023 on lipid accumulation and PPARα luciferase activity in HepG2 cells. Higher phosphorylated protein kinase B (pAkt)/Akt ratios and lower expression of gluconeogenesis genes were observed in the livers of ALS-L1023-treated mice. These results indicate that ALS-L1023 may inhibit obesity and improve insulin sensitivity in part through inhibition of hepatic lipid accumulation via hepatic PPARα activation. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/32549364/Lemon_Balm_Extract_ALS-L1023_Regulates_Obesity_and_Improves_Insulin_Sensitivity_via_Activation_of_Hepatic_PPARα_in_High-Fat_Diet-Fed_Obese_C57BL/6J_Mice L2 - https://www.mdpi.com/resolver?pii=ijms21124256 DB - PRIME DP - Unbound Medicine ER -
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