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Biochemical and immunochemical characterization of venoms from snakes of the genus Agkistrodon.
Toxicon X. 2019 Oct; 4:100013.TX

Abstract

In the present work, venoms from five species of the genus Agkistrodon were evaluated in terms of their enzymatic (Phospholipase A2 and caseinolytic) and biological (edema forming, hemorrhagic, procoagulant and lethal) effects. Horses were used to produce monovalent hyperimmune sera against each of three venoms (A. bilineatus, A. contortrix and A. piscivorus) and their neutralizing potency, expressed as Median Effective Dose (ED50), was determined against the venoms of all five species. In terms of PLA2 and caseinolytic activities, all venoms are extremely homogeneous. PLA2 activity is high, while caseinolytic activity is low when in contrast with that of the rattlesnake Crotalus simus. On the other hand, biological activities showed marked interspecific differences, particularly between the species from Mexico and those from the United States. Mexican species displayed higher edema-forming, hemorrhagic and lethal effects than US species, while none of the species studied presented procoagulant activity. All three monovalent hyperimmune sera showed good neutralizing potency against the analyzed venoms. Nonetheless, we observed relevant immunochemical differences among the venoms using ELISA and Western Blot assays. We conclude that the venoms of A. piscivorus (USA) and A. bilineatus would be ideal to use as immunogens for the production of a polyvalent antivenom with good neutralizing potency against the venoms of all the species of the genus.

Authors+Show Affiliations

Instituto de Biotecnología, Universidad Nacional Autónoma de México. Av. Universidad # 2001 Colonia Chamilpa. CP: 62210. Cuernavaca, Morelos, Mexico. Facultad de Ciencias Biológicas, Universidad Autónoma del Estado de Morelos. Av. Universidad # 2001 Colonia Chamilpa. CP: 62210. Cuernavaca, Morelos, Mexico.Instituto de Biotecnología, Universidad Nacional Autónoma de México. Av. Universidad # 2001 Colonia Chamilpa. CP: 62210. Cuernavaca, Morelos, Mexico.Instituto de Biotecnología, Universidad Nacional Autónoma de México. Av. Universidad # 2001 Colonia Chamilpa. CP: 62210. Cuernavaca, Morelos, Mexico.Instituto de Biotecnología, Universidad Nacional Autónoma de México. Av. Universidad # 2001 Colonia Chamilpa. CP: 62210. Cuernavaca, Morelos, Mexico.Instituto de Biotecnología, Universidad Nacional Autónoma de México. Av. Universidad # 2001 Colonia Chamilpa. CP: 62210. Cuernavaca, Morelos, Mexico.Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán. Km 15.5, Carretera Mérida-Xmatkuil. C.P: 97315. Mérida, Yucatán, Mexico. Unidad de Manejo para la Conservación de la Vida Silvestre Tsáab Kaan. Km. 2.8, Carretera Baca-Dzemul, C.P. 97450. Baca, Yucatán, Mexico.Facultad de Medicina. Universidad Autónoma del Estado de Morelos. Calle Leñeros S/N, Colonia Vista Hermosa. CP: 62290. Cuernavaca, Morelos, Mexico.Instituto de Biotecnología, Universidad Nacional Autónoma de México. Av. Universidad # 2001 Colonia Chamilpa. CP: 62210. Cuernavaca, Morelos, Mexico.Instituto de Biotecnología, Universidad Nacional Autónoma de México. Av. Universidad # 2001 Colonia Chamilpa. CP: 62210. Cuernavaca, Morelos, Mexico.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32550570

Citation

Román-Domínguez, Luis, et al. "Biochemical and Immunochemical Characterization of Venoms From Snakes of the Genus Agkistrodon." Toxicon: X, vol. 4, 2019, p. 100013.
Román-Domínguez L, Neri-Castro E, Vázquez López H, et al. Biochemical and immunochemical characterization of venoms from snakes of the genus Agkistrodon. Toxicon X. 2019;4:100013.
Román-Domínguez, L., Neri-Castro, E., Vázquez López, H., García-Osorio, B., Archundia, I. G., Ortiz-Medina, J. A., Petricevich, V. L., Alagón, A., & Bénard-Valle, M. (2019). Biochemical and immunochemical characterization of venoms from snakes of the genus Agkistrodon. Toxicon: X, 4, 100013. https://doi.org/10.1016/j.toxcx.2019.100013
Román-Domínguez L, et al. Biochemical and Immunochemical Characterization of Venoms From Snakes of the Genus Agkistrodon. Toxicon X. 2019;4:100013. PubMed PMID: 32550570.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biochemical and immunochemical characterization of venoms from snakes of the genus Agkistrodon. AU - Román-Domínguez,Luis, AU - Neri-Castro,Edgar, AU - Vázquez López,Hilda, AU - García-Osorio,Belem, AU - Archundia,Irving G, AU - Ortiz-Medina,Javier A, AU - Petricevich,Vera L, AU - Alagón,Alejandro, AU - Bénard-Valle,Melisa, Y1 - 2019/08/02/ PY - 2019/04/11/received PY - 2019/06/28/revised PY - 2019/07/23/accepted PY - 2020/6/19/entrez PY - 2020/6/19/pubmed PY - 2020/6/19/medline KW - Agkistrodon venoms KW - Antivenom neutralization KW - Edema KW - Hemorrhage KW - Immunochemistry SP - 100013 EP - 100013 JF - Toxicon: X JO - Toxicon X VL - 4 N2 - In the present work, venoms from five species of the genus Agkistrodon were evaluated in terms of their enzymatic (Phospholipase A2 and caseinolytic) and biological (edema forming, hemorrhagic, procoagulant and lethal) effects. Horses were used to produce monovalent hyperimmune sera against each of three venoms (A. bilineatus, A. contortrix and A. piscivorus) and their neutralizing potency, expressed as Median Effective Dose (ED50), was determined against the venoms of all five species. In terms of PLA2 and caseinolytic activities, all venoms are extremely homogeneous. PLA2 activity is high, while caseinolytic activity is low when in contrast with that of the rattlesnake Crotalus simus. On the other hand, biological activities showed marked interspecific differences, particularly between the species from Mexico and those from the United States. Mexican species displayed higher edema-forming, hemorrhagic and lethal effects than US species, while none of the species studied presented procoagulant activity. All three monovalent hyperimmune sera showed good neutralizing potency against the analyzed venoms. Nonetheless, we observed relevant immunochemical differences among the venoms using ELISA and Western Blot assays. We conclude that the venoms of A. piscivorus (USA) and A. bilineatus would be ideal to use as immunogens for the production of a polyvalent antivenom with good neutralizing potency against the venoms of all the species of the genus. SN - 2590-1710 UR - https://www.unboundmedicine.com/medline/citation/32550570/Biochemical_and_immunochemical_characterization_of_venoms_from_snakes_of_the_genus_Agkistrodon L2 - https://linkinghub.elsevier.com/retrieve/pii/S2590-1710(19)30010-4 DB - PRIME DP - Unbound Medicine ER -