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Isolation and Characterization of Novel Trichomonas gallinae Ribotypes Infecting Domestic and Wild Birds in Riyadh, Saudi Arabia.
Avian Dis. 2020 06; 64(2):130-134.AD

Abstract

Trichomonas gallinae, a single-celled protozoan parasite, is a causative agent of the disease trichomonosis, which is distributed worldwide and has recently been highlighted as a pandemic threat to several wild bird species. The aim of this study was to determine the prevalence and genotypic diversity of T. gallinae in Riyadh, Saudi Arabia. For this purpose, 273 oral swab samples from different bird species (feral pigeon Columba livia, common mynah Acridotheres tristis, chicken Gallus gallus domesticus, turkey Meleagris gallopavo, and ducks Anatidae) were collected and tested for T. gallinae infection with InPouch™ TV culture kits. The results showed that the overall prevalence of T. gallinae in these samples was 26.4% (n = 72). The PCRs were used to detect the internal transcribed spacer (ITS) region of T. gallinae, and the results of the sequence analysis indicated genetic variation. Among 48 sequences, we found 15 different ribotypes, of which 12 were novel. Three had been previously described as ribotypes A, C, and II. To our knowledge, this study demonstrated the presence of T. gallinae strain diversity in Saudi Arabian birds for the first time and revealed that ribotypes A and C are predominant among Riyadh birds.

Authors+Show Affiliations

Department of Zoology, King Saud University, College of Science, Riyadh-11451, Saudi Arabia.Department of Zoology, King Saud University, College of Science, Riyadh-11451, Saudi Arabia, afrefaei@ksu.edu.sa.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32550612

Citation

Albeshr, Mohammed F., and Abdulwahed F. Alrefaei. "Isolation and Characterization of Novel Trichomonas Gallinae Ribotypes Infecting Domestic and Wild Birds in Riyadh, Saudi Arabia." Avian Diseases, vol. 64, no. 2, 2020, pp. 130-134.
Albeshr MF, Alrefaei AF. Isolation and Characterization of Novel Trichomonas gallinae Ribotypes Infecting Domestic and Wild Birds in Riyadh, Saudi Arabia. Avian Dis. 2020;64(2):130-134.
Albeshr, M. F., & Alrefaei, A. F. (2020). Isolation and Characterization of Novel Trichomonas gallinae Ribotypes Infecting Domestic and Wild Birds in Riyadh, Saudi Arabia. Avian Diseases, 64(2), 130-134. https://doi.org/10.1637/0005-2086-64.2.130
Albeshr MF, Alrefaei AF. Isolation and Characterization of Novel Trichomonas Gallinae Ribotypes Infecting Domestic and Wild Birds in Riyadh, Saudi Arabia. Avian Dis. 2020;64(2):130-134. PubMed PMID: 32550612.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and Characterization of Novel Trichomonas gallinae Ribotypes Infecting Domestic and Wild Birds in Riyadh, Saudi Arabia. AU - Albeshr,Mohammed F, AU - Alrefaei,Abdulwahed F, PY - 2019/09/27/received PY - 2019/12/30/accepted PY - 2020/6/19/entrez PY - 2020/6/19/pubmed PY - 2020/6/19/medline KW - ITS region KW - Trichomonas gallinae KW - columbid KW - genotypic diversity KW - prevalence KW - protozoan parasite KW - ribotype KW - trichomonads KW - trichomoniasis KW - trichomonosis SP - 130 EP - 134 JF - Avian diseases JO - Avian Dis. VL - 64 IS - 2 N2 - Trichomonas gallinae, a single-celled protozoan parasite, is a causative agent of the disease trichomonosis, which is distributed worldwide and has recently been highlighted as a pandemic threat to several wild bird species. The aim of this study was to determine the prevalence and genotypic diversity of T. gallinae in Riyadh, Saudi Arabia. For this purpose, 273 oral swab samples from different bird species (feral pigeon Columba livia, common mynah Acridotheres tristis, chicken Gallus gallus domesticus, turkey Meleagris gallopavo, and ducks Anatidae) were collected and tested for T. gallinae infection with InPouch™ TV culture kits. The results showed that the overall prevalence of T. gallinae in these samples was 26.4% (n = 72). The PCRs were used to detect the internal transcribed spacer (ITS) region of T. gallinae, and the results of the sequence analysis indicated genetic variation. Among 48 sequences, we found 15 different ribotypes, of which 12 were novel. Three had been previously described as ribotypes A, C, and II. To our knowledge, this study demonstrated the presence of T. gallinae strain diversity in Saudi Arabian birds for the first time and revealed that ribotypes A and C are predominant among Riyadh birds. SN - 1938-4351 UR - https://www.unboundmedicine.com/medline/citation/32550612/Isolation_and_Characterization_of_Novel_Trichomonas_gallinae_Ribotypes_Infecting_Domestic_and_Wild_Birds_in_Riyadh,_Saudi_Arabia L2 - https://doi.org/10.1637/0005-2086-64.2.130 DB - PRIME DP - Unbound Medicine ER -
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