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Identification and characterization of caleosin in Cycas revoluta pollen.
Plant Signal Behav. 2020 08 02; 15(8):1779486.PS

Abstract

Oil bodies are essential energy storage organelles that are generally present in the seeds of plants. Caleosin protein has been identified in the seed oil bodies of Cycas revolutaseed. In this study, mature Cycas revoluta pollen grains were collected from cycad elliptical cones. However, the isolation and identification of oil bodies protein from mature Cycas revoluta grains have never been experimentally determined. Ultrastructural studies have shown that the oil bodies were present in pollen Cycas revoluta grains. Lipid analysis showed that oil bodies are predominantly composed of triacylglycerol. Complete cDNA fragments encoding the caleosin were obtained by PCR cloning. Phylogenetic tree analyzes showed that cycad pollen caleosin is closely related to the caleosin of Cycas revoluta seeds. Fresh Cycas revoluta pollen grains were allowed to germinate for 48 h in a germination medium containing 2% sucrose and 0.01% H3BO3. Pollen germination were recorded periodically from day one to day 10 and the results showed that the Cycas revoluta pollen elongate the tube and increasing of triacylglycerol(TAG) after 4 days.

Authors+Show Affiliations

Department of Plant Biology & Pathology, Rutgers University , New Brunswick, USA.Department of Biotechnology, National Formosa University , Yunlin, Taiwan.Department of Biotechnology, National Formosa University , Yunlin, Taiwan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32552503

Citation

Pasaribu, Buntora, et al. "Identification and Characterization of Caleosin in Cycas Revoluta Pollen." Plant Signaling & Behavior, vol. 15, no. 8, 2020, p. 1779486.
Pasaribu B, Fu JH, Jiang PL. Identification and characterization of caleosin in Cycas revoluta pollen. Plant Signal Behav. 2020;15(8):1779486.
Pasaribu, B., Fu, J. H., & Jiang, P. L. (2020). Identification and characterization of caleosin in Cycas revoluta pollen. Plant Signaling & Behavior, 15(8), 1779486. https://doi.org/10.1080/15592324.2020.1779486
Pasaribu B, Fu JH, Jiang PL. Identification and Characterization of Caleosin in Cycas Revoluta Pollen. Plant Signal Behav. 2020 08 2;15(8):1779486. PubMed PMID: 32552503.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of caleosin in Cycas revoluta pollen. AU - Pasaribu,Buntora, AU - Fu,Jou-Hsuan, AU - Jiang,Pei-Luen, Y1 - 2020/06/17/ PY - 2020/6/20/pubmed PY - 2021/5/29/medline PY - 2020/6/20/entrez KW - Cycas revoluta KW - Caleosin KW - oil body KW - pollen SP - 1779486 EP - 1779486 JF - Plant signaling & behavior JO - Plant Signal Behav VL - 15 IS - 8 N2 - Oil bodies are essential energy storage organelles that are generally present in the seeds of plants. Caleosin protein has been identified in the seed oil bodies of Cycas revolutaseed. In this study, mature Cycas revoluta pollen grains were collected from cycad elliptical cones. However, the isolation and identification of oil bodies protein from mature Cycas revoluta grains have never been experimentally determined. Ultrastructural studies have shown that the oil bodies were present in pollen Cycas revoluta grains. Lipid analysis showed that oil bodies are predominantly composed of triacylglycerol. Complete cDNA fragments encoding the caleosin were obtained by PCR cloning. Phylogenetic tree analyzes showed that cycad pollen caleosin is closely related to the caleosin of Cycas revoluta seeds. Fresh Cycas revoluta pollen grains were allowed to germinate for 48 h in a germination medium containing 2% sucrose and 0.01% H3BO3. Pollen germination were recorded periodically from day one to day 10 and the results showed that the Cycas revoluta pollen elongate the tube and increasing of triacylglycerol(TAG) after 4 days. SN - 1559-2324 UR - https://www.unboundmedicine.com/medline/citation/32552503/Identification_and_characterization_of_caleosin_in_Cycas_revoluta_pollen_ L2 - https://www.tandfonline.com/doi/full/10.1080/15592324.2020.1779486 DB - PRIME DP - Unbound Medicine ER -