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Characterization and anti-uterine tumor effect of extract from Prunella vulgaris L.
BMC Complement Med Ther. 2020 Jun 18; 20(1):189.BC

Abstract

BACKGROUND

The flowers and dried fruit spikes of Prunella vulgaris L. (P. vulgaris L.) have been widely used in traditional Chinese medicine and food. P. vulgaris L. is regarded as a good option for treating uterine myoma (UM). However, scientific evidence of anti-UM activity of the extract of P. vulgaris L. (PVE) is lacking. The present study aimed to characterize the chemical composition of PVE and evaluate the pharmacodynamics and mechanism of PVE against UM.

METHODS

The chemical composition of PVE was analyzed by GC-MS. MTT was used to screen and evaluate cell proliferation and toxicity. Double fluorescence flow cytometry method were used to determine the apoptosis and cell cycle progression of UM cells under PVE treatment. The anti-UM activity of PVE was investigated by using a specific-pathogen-free (SPF) rat model of UM. TUNEL staining was used to detect the apoptosis of UM cells. The concentrations of estrogen and progesterone in the serum of SPF rats were detected by ELISA. The expression levels of PCNA, estrogen receptor alpha, estrogen receptor beta, progesterone receptor, survivin, caspase-3, Bax and Bcl-2 in the uterus of SPF rats was detected by immunohistochemistry (IHC).

RESULTS

The extraction rate of PVE was 8.1%. The main components were squalene (28.3%), linoleic acid (9.96%), linolenic acid (9.95%), stearic acid (6.26%) and oleic acid (5.51%). In vitro, PVE had significant anti-human UM cell activity, exhibited no drug toxicity, promoted the apoptosis of human UM cells, and inhibited the transition of UM cells from the G0/G1 stage into the G2 stage, in which DNA replication occurs. In vivo, PVE had significant anti-UM activity. PVE decreased the concentrations of estrogen and progesterone and downregulated the expression levels of the estrogen and progesterone receptors through the estrogen signaling pathway. PVE also promoted the apoptosis of UM cells by downregulating the expression levels of the survivin and Bcl-2 proteins and upregulating the expression levels of caspase-3 and Bax through the mitochondria-mediated apoptotic pathway.

CONCLUSION

PVE has marked anti-UM activity. PVE can be used as an ideal candidate drug to treat UM.

Authors+Show Affiliations

Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, College of Pharmacy, Hunan University of Chinese Medicine, No.300 Xueshi Road, Changsha, 410208, People's Republic of China.Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, College of Pharmacy, Hunan University of Chinese Medicine, No.300 Xueshi Road, Changsha, 410208, People's Republic of China.Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, College of Pharmacy, Hunan University of Chinese Medicine, No.300 Xueshi Road, Changsha, 410208, People's Republic of China.China Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, People's Republic of China.Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, College of Pharmacy, Hunan University of Chinese Medicine, No.300 Xueshi Road, Changsha, 410208, People's Republic of China.Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, College of Pharmacy, Hunan University of Chinese Medicine, No.300 Xueshi Road, Changsha, 410208, People's Republic of China.Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, College of Pharmacy, Hunan University of Chinese Medicine, No.300 Xueshi Road, Changsha, 410208, People's Republic of China.Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, School of Pharmacy and Life Science, University of South China, Hengyang, 421001, People's Republic of China.Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, College of Pharmacy, Hunan University of Chinese Medicine, No.300 Xueshi Road, Changsha, 410208, People's Republic of China.Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, College of Pharmacy, Hunan University of Chinese Medicine, No.300 Xueshi Road, Changsha, 410208, People's Republic of China. dfliao@hnucm.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32552673

Citation

Lin, Yan, et al. "Characterization and Anti-uterine Tumor Effect of Extract From Prunella Vulgaris L." BMC Complementary Medicine and Therapies, vol. 20, no. 1, 2020, p. 189.
Lin Y, Yang C, Tang J, et al. Characterization and anti-uterine tumor effect of extract from Prunella vulgaris L. BMC Complement Med Ther. 2020;20(1):189.
Lin, Y., Yang, C., Tang, J., Li, C., Zhang, Z. M., Xia, B. H., Li, Y. M., He, Q. Z., Lin, L. M., & Liao, D. F. (2020). Characterization and anti-uterine tumor effect of extract from Prunella vulgaris L. BMC Complementary Medicine and Therapies, 20(1), 189. https://doi.org/10.1186/s12906-020-02986-5
Lin Y, et al. Characterization and Anti-uterine Tumor Effect of Extract From Prunella Vulgaris L. BMC Complement Med Ther. 2020 Jun 18;20(1):189. PubMed PMID: 32552673.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and anti-uterine tumor effect of extract from Prunella vulgaris L. AU - Lin,Yan, AU - Yang,Chao, AU - Tang,Jie, AU - Li,Chun, AU - Zhang,Zhi-Min, AU - Xia,Bo-Hou, AU - Li,Ya-Mei, AU - He,Qing-Zhi, AU - Lin,Li-Mei, AU - Liao,Duan-Fang, Y1 - 2020/06/18/ PY - 2019/09/15/received PY - 2020/06/10/accepted PY - 2020/6/20/entrez PY - 2020/6/20/pubmed PY - 2020/6/20/medline KW - Apoptosis KW - GC-MS KW - Prunella vulgaris L. KW - Uterine Myoma SP - 189 EP - 189 JF - BMC complementary medicine and therapies JO - BMC Complement Med Ther VL - 20 IS - 1 N2 - BACKGROUND: The flowers and dried fruit spikes of Prunella vulgaris L. (P. vulgaris L.) have been widely used in traditional Chinese medicine and food. P. vulgaris L. is regarded as a good option for treating uterine myoma (UM). However, scientific evidence of anti-UM activity of the extract of P. vulgaris L. (PVE) is lacking. The present study aimed to characterize the chemical composition of PVE and evaluate the pharmacodynamics and mechanism of PVE against UM. METHODS: The chemical composition of PVE was analyzed by GC-MS. MTT was used to screen and evaluate cell proliferation and toxicity. Double fluorescence flow cytometry method were used to determine the apoptosis and cell cycle progression of UM cells under PVE treatment. The anti-UM activity of PVE was investigated by using a specific-pathogen-free (SPF) rat model of UM. TUNEL staining was used to detect the apoptosis of UM cells. The concentrations of estrogen and progesterone in the serum of SPF rats were detected by ELISA. The expression levels of PCNA, estrogen receptor alpha, estrogen receptor beta, progesterone receptor, survivin, caspase-3, Bax and Bcl-2 in the uterus of SPF rats was detected by immunohistochemistry (IHC). RESULTS: The extraction rate of PVE was 8.1%. The main components were squalene (28.3%), linoleic acid (9.96%), linolenic acid (9.95%), stearic acid (6.26%) and oleic acid (5.51%). In vitro, PVE had significant anti-human UM cell activity, exhibited no drug toxicity, promoted the apoptosis of human UM cells, and inhibited the transition of UM cells from the G0/G1 stage into the G2 stage, in which DNA replication occurs. In vivo, PVE had significant anti-UM activity. PVE decreased the concentrations of estrogen and progesterone and downregulated the expression levels of the estrogen and progesterone receptors through the estrogen signaling pathway. PVE also promoted the apoptosis of UM cells by downregulating the expression levels of the survivin and Bcl-2 proteins and upregulating the expression levels of caspase-3 and Bax through the mitochondria-mediated apoptotic pathway. CONCLUSION: PVE has marked anti-UM activity. PVE can be used as an ideal candidate drug to treat UM. SN - 2662-7671 UR - https://www.unboundmedicine.com/medline/citation/32552673/Characterization_and_anti-uterine_tumor_effect_of_extract_from_Prunella_vulgaris_L L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32552673/ DB - PRIME DP - Unbound Medicine ER -
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