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Draft genome of the European medicinal leech Hirudo medicinalis (Annelida, Clitellata, Hirudiniformes) with emphasis on anticoagulants.
Sci Rep. 2020 Jun 18; 10(1):9885.SR

Abstract

The European medicinal leech has been used for medicinal purposes for millennia, and continues to be used today in modern hospital settings. Its utility is granted by the extremely potent anticoagulation factors that the leech secretes into the incision wound during feeding and, although a handful of studies have targeted certain anticoagulants, the full range of anticoagulation factors expressed by this species remains unknown. Here, we present the first draft genome of the European medicinal leech, Hirudo medicinalis, and estimate that we have sequenced between 79-94% of the full genome. Leveraging these data, we searched for anticoagulation factors across the genome of H. medicinalis. Following orthology determination through a series of BLAST searches, as well as phylogenetic analyses, we estimate that fully 15 different known anticoagulation factors are utilized by the species, and that 17 other proteins that have been linked to antihemostasis are also present in the genome. We underscore the utility of the draft genome for comparative studies of leeches and discuss our results in an evolutionary context.

Authors+Show Affiliations

Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, ON, M5S 2C6, Canada. skvist@rom.on.ca. Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, ON, M5S 2B4, Canada. skvist@rom.on.ca.Centre for Microbiology and Environmental Systems Science, University of Vienna, 1090, Vienna, Austria.Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, ON, M5S 2C6, Canada. Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, ON, M5S 2B4, Canada.Department of Biology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia.Division of invertebrate Zoology, American Museum of Natural History, 79th Street @ Central Park West, New York, NY, 10025, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32555498

Citation

Kvist, Sebastian, et al. "Draft Genome of the European Medicinal Leech Hirudo Medicinalis (Annelida, Clitellata, Hirudiniformes) With Emphasis On Anticoagulants." Scientific Reports, vol. 10, no. 1, 2020, p. 9885.
Kvist S, Manzano-Marín A, de Carle D, et al. Draft genome of the European medicinal leech Hirudo medicinalis (Annelida, Clitellata, Hirudiniformes) with emphasis on anticoagulants. Sci Rep. 2020;10(1):9885.
Kvist, S., Manzano-Marín, A., de Carle, D., Trontelj, P., & Siddall, M. E. (2020). Draft genome of the European medicinal leech Hirudo medicinalis (Annelida, Clitellata, Hirudiniformes) with emphasis on anticoagulants. Scientific Reports, 10(1), 9885. https://doi.org/10.1038/s41598-020-66749-5
Kvist S, et al. Draft Genome of the European Medicinal Leech Hirudo Medicinalis (Annelida, Clitellata, Hirudiniformes) With Emphasis On Anticoagulants. Sci Rep. 2020 Jun 18;10(1):9885. PubMed PMID: 32555498.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Draft genome of the European medicinal leech Hirudo medicinalis (Annelida, Clitellata, Hirudiniformes) with emphasis on anticoagulants. AU - Kvist,Sebastian, AU - Manzano-Marín,Alejandro, AU - de Carle,Danielle, AU - Trontelj,Peter, AU - Siddall,Mark E, Y1 - 2020/06/18/ PY - 2020/02/18/received PY - 2020/04/28/accepted PY - 2020/6/20/entrez PY - 2020/6/20/pubmed PY - 2020/6/20/medline SP - 9885 EP - 9885 JF - Scientific reports JO - Sci Rep VL - 10 IS - 1 N2 - The European medicinal leech has been used for medicinal purposes for millennia, and continues to be used today in modern hospital settings. Its utility is granted by the extremely potent anticoagulation factors that the leech secretes into the incision wound during feeding and, although a handful of studies have targeted certain anticoagulants, the full range of anticoagulation factors expressed by this species remains unknown. Here, we present the first draft genome of the European medicinal leech, Hirudo medicinalis, and estimate that we have sequenced between 79-94% of the full genome. Leveraging these data, we searched for anticoagulation factors across the genome of H. medicinalis. Following orthology determination through a series of BLAST searches, as well as phylogenetic analyses, we estimate that fully 15 different known anticoagulation factors are utilized by the species, and that 17 other proteins that have been linked to antihemostasis are also present in the genome. We underscore the utility of the draft genome for comparative studies of leeches and discuss our results in an evolutionary context. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/32555498/Draft_genome_of_the_European_medicinal_leech_Hirudo_medicinalis_(Annelida,_Clitellata,_Hirudiniformes)_with_emphasis_on_anticoagulants L2 - http://dx.doi.org/10.1038/s41598-020-66749-5 DB - PRIME DP - Unbound Medicine ER -
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