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PPAR-α Agonist Fenofibrate Ameliorates Oxidative Stress in Testicular Tissue of Diabetic Rats.
Crit Rev Eukaryot Gene Expr. 2020; 30(2):93-100.CR

Abstract

BACKGROUND

Oxidative stress has the potential to induce impotence, especially in diabetic patients. Peroxisome proliferator-activated receptor alpha (PPAR-α) agonists can potentiate antioxidants in a wide variety of tissues. However, no available evidence exists showing a direct antioxidant effect on testicular tissue in the setting of diabetes. Therefore, the aim of this study was to evaluate whether PPAR-α agonists can act directly to protect testicular tissue from oxidative damage.

METHODS

Male Wistar rats (180-200 g) were randomly allocated into four groups: normal control (N), normal treated (NF), diabetic (D), and diabetic treated (DF) (n = 6 for each group). Diabetes was induced by a single intravenous injection of streptozotocin STZ (40 mg/kg). Two treatment groups (diabetic and nondiabetic) were treated with fenofibrate daily for 8 weeks (80 mg/kg orally). At the end of 8 weeks, the animals were sacrificed and blood and testicular tissue samples collected. Nitrate, malondialdehyde, and glutathione levels, and the activity of superoxide dismutase and catalase enzymes were evaluated. The data were analyzed via two-way analysis of variance (ANOVA), with P < 0.05 taken as significant.

RESULTS

Diabetes significantly augmented free radicals, as attested by an increase in nitrate levels in testicular tissue, reduced activity of superoxide dismutase and catalase enzymes, and enhanced malondialdehyde content. These changes lead to oxidative stress in testicular tissues. Treatment with fenofibrate in the diabetic group improved oxidative stress by potentiation of antioxidant elements and a reduction in nitrate and malondialdehyde production.

CONCLUSION

Diabetes has a potent effect in promoting the development of oxidative damage in testicular tissue. The PPAR-a agonist fenofibrate improves the redox state and may prevent oxidative stress in the setting of diabetes-induced oxidative stress.

Authors+Show Affiliations

Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran.Department of Physiology and Biophysics, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Halal Research Center of IRI, FDA, Tehran, Iran; Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32558488

Citation

Yaribeygi, Habib, et al. "PPAR-α Agonist Fenofibrate Ameliorates Oxidative Stress in Testicular Tissue of Diabetic Rats." Critical Reviews in Eukaryotic Gene Expression, vol. 30, no. 2, 2020, pp. 93-100.
Yaribeygi H, Mohammadi MT, Jamialahmadi T, et al. PPAR-α Agonist Fenofibrate Ameliorates Oxidative Stress in Testicular Tissue of Diabetic Rats. Crit Rev Eukaryot Gene Expr. 2020;30(2):93-100.
Yaribeygi, H., Mohammadi, M. T., Jamialahmadi, T., & Sahebkar, A. (2020). PPAR-α Agonist Fenofibrate Ameliorates Oxidative Stress in Testicular Tissue of Diabetic Rats. Critical Reviews in Eukaryotic Gene Expression, 30(2), 93-100. https://doi.org/10.1615/CritRevEukaryotGeneExpr.2020027918
Yaribeygi H, et al. PPAR-α Agonist Fenofibrate Ameliorates Oxidative Stress in Testicular Tissue of Diabetic Rats. Crit Rev Eukaryot Gene Expr. 2020;30(2):93-100. PubMed PMID: 32558488.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PPAR-α Agonist Fenofibrate Ameliorates Oxidative Stress in Testicular Tissue of Diabetic Rats. AU - Yaribeygi,Habib, AU - Mohammadi,Mohammad Taghi, AU - Jamialahmadi,Tannaz, AU - Sahebkar,Amirhossein, PY - 2020/6/20/entrez SP - 93 EP - 100 JF - Critical reviews in eukaryotic gene expression JO - Crit. Rev. Eukaryot. Gene Expr. VL - 30 IS - 2 N2 - BACKGROUND: Oxidative stress has the potential to induce impotence, especially in diabetic patients. Peroxisome proliferator-activated receptor alpha (PPAR-α) agonists can potentiate antioxidants in a wide variety of tissues. However, no available evidence exists showing a direct antioxidant effect on testicular tissue in the setting of diabetes. Therefore, the aim of this study was to evaluate whether PPAR-α agonists can act directly to protect testicular tissue from oxidative damage. METHODS: Male Wistar rats (180-200 g) were randomly allocated into four groups: normal control (N), normal treated (NF), diabetic (D), and diabetic treated (DF) (n = 6 for each group). Diabetes was induced by a single intravenous injection of streptozotocin STZ (40 mg/kg). Two treatment groups (diabetic and nondiabetic) were treated with fenofibrate daily for 8 weeks (80 mg/kg orally). At the end of 8 weeks, the animals were sacrificed and blood and testicular tissue samples collected. Nitrate, malondialdehyde, and glutathione levels, and the activity of superoxide dismutase and catalase enzymes were evaluated. The data were analyzed via two-way analysis of variance (ANOVA), with P < 0.05 taken as significant. RESULTS: Diabetes significantly augmented free radicals, as attested by an increase in nitrate levels in testicular tissue, reduced activity of superoxide dismutase and catalase enzymes, and enhanced malondialdehyde content. These changes lead to oxidative stress in testicular tissues. Treatment with fenofibrate in the diabetic group improved oxidative stress by potentiation of antioxidant elements and a reduction in nitrate and malondialdehyde production. CONCLUSION: Diabetes has a potent effect in promoting the development of oxidative damage in testicular tissue. The PPAR-a agonist fenofibrate improves the redox state and may prevent oxidative stress in the setting of diabetes-induced oxidative stress. SN - 1045-4403 UR - https://www.unboundmedicine.com/medline/citation/32558488/PPAR-α_Agonist_Fenofibrate_Ameliorates_Oxidative_Stress_in_Testicular_Tissue_of_Diabetic_Rats L2 - http://www.begellhouse.com/journals/6dbf508d3b17c437,186e14b9417da291,06ad258143ceaac3.html DB - PRIME DP - Unbound Medicine ER -
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