Tags

Type your tag names separated by a space and hit enter

Research on the structural characteristics of a novel Chinese Iron Yam polysaccharide and its gastroprotection mechanism against ethanol-induced gastric mucosal lesion in a BALB/c mouse model.
Food Funct. 2020 Jul 01; 11(7):6054-6065.FF

Abstract

In this study, a triple-helix Chinese Iron Yam polysaccharide (CIYP) with a molecular weight of 1.67 × 103 kDa was obtained. The CIYP was extracted with deionized water followed by deproteination, decoloration and purification using anion-exchange chromatography and size exclusion chromatography. Its structural characteristics and micromorphology were investigated by GC-MS, periodate oxidation and Smith degradation, FT-IR, NMR spectroscopy, SEM and AFM. The results showed that CIYP is a catenarian polysaccharide composed of rhamnose, arabinose, mannose, glucose, galactose and galacturonic acid in the ratio of 1 : 1.33 : 8.31 : 2.83 : 1.12 : 2.62. Meanwhile, the gastric mucosa protective effect of CIYP on an ethanol-injured BALB/c mouse model was investigated. It was found that the preventive CIYP-treatment groups (200 and 400 mg kg-1 d-1) showed gastric mucosa protective effects on the BALB/c mouse model. The lesion index and lesion inhibition rate of the CIYP and cimetidine treatment groups were significantly altered compared with the ethanol-induced gastric mucosal lesion (GML) group. Moreover, the administration of CIYP showed definite effects of increasing the NO, PGE2 and EGF levels, and SOD activities, and reducing the MDA levels of gastric mucosa tissues to prevent gastric oxidative stress. Histopathological analysis indicated that the microscopic morphology of gastric mucosal tissues was changed after being damaged by ethanol and the damage was significantly reduced after CIYP administration. Finally, the western blot and quantitative real-time polymerase chain reaction (qRT-PCR) results provided comprehensive evidence that the CIYP could repress gastric inflammation through the reduction of IL-1β, TNF-α and IL-6, prevent gastric oxidative stress through the inhibition of lipid peroxides, and favor cell survival via downregulating the TAK1, MKK3, P-p38 and Bax levels and upregulating the protein expression levels, compared with the CIM group.

Authors+Show Affiliations

State Key Laboratory of Food Nutrition and Safety, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China. wangchunling@tust.edu.cn.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32558848

Citation

Guo, Mingzhu, et al. "Research On the Structural Characteristics of a Novel Chinese Iron Yam Polysaccharide and Its Gastroprotection Mechanism Against Ethanol-induced Gastric Mucosal Lesion in a BALB/c Mouse Model." Food & Function, vol. 11, no. 7, 2020, pp. 6054-6065.
Guo M, Yu H, Meng M, et al. Research on the structural characteristics of a novel Chinese Iron Yam polysaccharide and its gastroprotection mechanism against ethanol-induced gastric mucosal lesion in a BALB/c mouse model. Food Funct. 2020;11(7):6054-6065.
Guo, M., Yu, H., Meng, M., & Wang, C. (2020). Research on the structural characteristics of a novel Chinese Iron Yam polysaccharide and its gastroprotection mechanism against ethanol-induced gastric mucosal lesion in a BALB/c mouse model. Food & Function, 11(7), 6054-6065. https://doi.org/10.1039/c9fo02642h
Guo M, et al. Research On the Structural Characteristics of a Novel Chinese Iron Yam Polysaccharide and Its Gastroprotection Mechanism Against Ethanol-induced Gastric Mucosal Lesion in a BALB/c Mouse Model. Food Funct. 2020 Jul 1;11(7):6054-6065. PubMed PMID: 32558848.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Research on the structural characteristics of a novel Chinese Iron Yam polysaccharide and its gastroprotection mechanism against ethanol-induced gastric mucosal lesion in a BALB/c mouse model. AU - Guo,Mingzhu, AU - Yu,Haifen, AU - Meng,Meng, AU - Wang,Chunling, Y1 - 2020/06/19/ PY - 2020/6/20/pubmed PY - 2020/6/20/medline PY - 2020/6/20/entrez SP - 6054 EP - 6065 JF - Food & function JO - Food Funct VL - 11 IS - 7 N2 - In this study, a triple-helix Chinese Iron Yam polysaccharide (CIYP) with a molecular weight of 1.67 × 103 kDa was obtained. The CIYP was extracted with deionized water followed by deproteination, decoloration and purification using anion-exchange chromatography and size exclusion chromatography. Its structural characteristics and micromorphology were investigated by GC-MS, periodate oxidation and Smith degradation, FT-IR, NMR spectroscopy, SEM and AFM. The results showed that CIYP is a catenarian polysaccharide composed of rhamnose, arabinose, mannose, glucose, galactose and galacturonic acid in the ratio of 1 : 1.33 : 8.31 : 2.83 : 1.12 : 2.62. Meanwhile, the gastric mucosa protective effect of CIYP on an ethanol-injured BALB/c mouse model was investigated. It was found that the preventive CIYP-treatment groups (200 and 400 mg kg-1 d-1) showed gastric mucosa protective effects on the BALB/c mouse model. The lesion index and lesion inhibition rate of the CIYP and cimetidine treatment groups were significantly altered compared with the ethanol-induced gastric mucosal lesion (GML) group. Moreover, the administration of CIYP showed definite effects of increasing the NO, PGE2 and EGF levels, and SOD activities, and reducing the MDA levels of gastric mucosa tissues to prevent gastric oxidative stress. Histopathological analysis indicated that the microscopic morphology of gastric mucosal tissues was changed after being damaged by ethanol and the damage was significantly reduced after CIYP administration. Finally, the western blot and quantitative real-time polymerase chain reaction (qRT-PCR) results provided comprehensive evidence that the CIYP could repress gastric inflammation through the reduction of IL-1β, TNF-α and IL-6, prevent gastric oxidative stress through the inhibition of lipid peroxides, and favor cell survival via downregulating the TAK1, MKK3, P-p38 and Bax levels and upregulating the protein expression levels, compared with the CIM group. SN - 2042-650X UR - https://www.unboundmedicine.com/medline/citation/32558848/Research_on_the_structural_characteristics_of_a_novel_Chinese_Iron_Yam_polysaccharide_and_its_gastroprotection_mechanism_against_ethanol-induced_gastric_mucosal_lesion_in_a_BALB/c_mouse_model L2 - https://doi.org/10.1039/c9fo02642h DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.