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Mass spectrometry analysis of urinary methylmalonic acid to screen for metabolic vitamin B12 deficiency in older adults.
Bioanalysis. 2020 May; 12(10):693-705.B

Abstract

Aim: Vitamin B12 deficiency is characterized metabolically by increased serum and urine methylmalonic acid (MMA). Urinary MMA/creatinine ratio is suggested for screening for metabolic vitamin B12 deficiency in older populations. Results: A UPLC-MS/MS method for the analysis of urinary MMA and creatinine was developed/validated. A good separation of MMA from succinic acid, its structural isomer, was achieved. Intra- and interday accuracy biases and precision coefficients were all ≤6.3% for MMA and creatinine. Urine and serum samples of 34 individuals of the NuAge Biobank were analyzed for technical comparisons showing that urinary MMA/creatinine ratios by UPLC-MS/MS strongly correlated with GC-MS values, and with serum MMA values. Conclusion: The UPLC-MS/MS method developed is rapid/reliable for the analysis of urinary MMA/creatinine ratios.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Boutin M
Division of Medical Genetics, Department of Pediatrics, Centre de Recherche-CHUS, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke J1H 5N4, QC, Canada.
Presse N
Research Center on Aging, Centre Intégré Universitaire de santé et de Services Sociaux de l'Estrie-CHUS, Sherbrooke J1H 4C4, QC, Canada. Department of Community Health Sciences, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke J1H 5N4, QC, Canada. Centre de recherche, Institut Universitaire de Gériatrie de Montréal, Montréal H3W 1W5, QC, Canada.
Martineau T
Division of Medical Genetics, Department of Pediatrics, Centre de Recherche-CHUS, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke J1H 5N4, QC, Canada.
Perreault A
Research Center on Aging, Centre Intégré Universitaire de santé et de Services Sociaux de l'Estrie-CHUS, Sherbrooke J1H 4C4, QC, Canada.
Gaudreau P
Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montréal H2X 0A9, QC, Canada. Département de Médecine, Faculté de Médecine, Université de Montréal, Montréal H2X 0A9, QC, Canada.
Auray-Blais C
Division of Medical Genetics, Department of Pediatrics, Centre de Recherche-CHUS, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke J1H 5N4, QC, Canada.

MeSH

AgedAged, 80 and overChromatography, High Pressure LiquidDrug Evaluation, PreclinicalFemaleGas Chromatography-Mass SpectrometryHumansMaleMethylmalonic AcidTandem Mass SpectrometryVitamin B 12 Deficiency

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32559133

Citation

Boutin, Michel, et al. "Mass Spectrometry Analysis of Urinary Methylmalonic Acid to Screen for Metabolic Vitamin B12 Deficiency in Older Adults." Bioanalysis, vol. 12, no. 10, 2020, pp. 693-705.
Boutin M, Presse N, Martineau T, et al. Mass spectrometry analysis of urinary methylmalonic acid to screen for metabolic vitamin B12 deficiency in older adults. Bioanalysis. 2020;12(10):693-705.
Boutin, M., Presse, N., Martineau, T., Perreault, A., Gaudreau, P., & Auray-Blais, C. (2020). Mass spectrometry analysis of urinary methylmalonic acid to screen for metabolic vitamin B12 deficiency in older adults. Bioanalysis, 12(10), 693-705. https://doi.org/10.4155/bio-2020-0106
Boutin M, et al. Mass Spectrometry Analysis of Urinary Methylmalonic Acid to Screen for Metabolic Vitamin B12 Deficiency in Older Adults. Bioanalysis. 2020;12(10):693-705. PubMed PMID: 32559133.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mass spectrometry analysis of urinary methylmalonic acid to screen for metabolic vitamin B12 deficiency in older adults. AU - Boutin,Michel, AU - Presse,Nancy, AU - Martineau,Tristan, AU - Perreault,Audrey, AU - Gaudreau,Pierrette, AU - Auray-Blais,Christiane, Y1 - 2020/06/19/ PY - 2020/6/20/pubmed PY - 2021/2/4/medline PY - 2020/6/20/entrez KW - MMA KW - UPLC–MS/MS KW - creatinine KW - liquid chromatography KW - mass spectrometry KW - method development and validation KW - methylmalonic acid KW - urine KW - vitamin B12 SP - 693 EP - 705 JF - Bioanalysis JO - Bioanalysis VL - 12 IS - 10 N2 - Aim: Vitamin B12 deficiency is characterized metabolically by increased serum and urine methylmalonic acid (MMA). Urinary MMA/creatinine ratio is suggested for screening for metabolic vitamin B12 deficiency in older populations. Results: A UPLC-MS/MS method for the analysis of urinary MMA and creatinine was developed/validated. A good separation of MMA from succinic acid, its structural isomer, was achieved. Intra- and interday accuracy biases and precision coefficients were all ≤6.3% for MMA and creatinine. Urine and serum samples of 34 individuals of the NuAge Biobank were analyzed for technical comparisons showing that urinary MMA/creatinine ratios by UPLC-MS/MS strongly correlated with GC-MS values, and with serum MMA values. Conclusion: The UPLC-MS/MS method developed is rapid/reliable for the analysis of urinary MMA/creatinine ratios. SN - 1757-6199 UR - https://www.unboundmedicine.com/medline/citation/32559133/Mass_spectrometry_analysis_of_urinary_methylmalonic_acid_to_screen_for_metabolic_vitamin_B12_deficiency_in_older_adults_ DB - PRIME DP - Unbound Medicine ER -