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A high-throughput assay for screening environmental pollutants and drugs impairing predator avoidance in Daphnia magna.
Sci Total Environ. 2020 Jun 06; 740:140045.ST

Abstract

This study addresses short-term habituation of the escape response in the aquatic crustacean Daphnia magna evoked by sudden changes in light intensity, using a high-throughput system. Daphnia magna exhibits a marked phototactic behaviour and swim away from light to avoid predation by fish. Currently, there is no information available on the habituation of this phototactic response. The Daphnia photomotor response assay (DPRA) measures the distance moved after a sudden increase in light intensity. Using DPRA, it is possible to determine not only the magnitude of the phototactic response, but also its habituation after repetitive cycles of light and darkness. The progressive reduction observed in response to a series of light stimuli in the proposed assay meet the criteria for habituation. Most cholinergic and serotonergic modulators enhanced photomotor responses and reduced habituation. Dopaminergic and histaminergic modulators also reduced habituation, whereas diazepam was the only compound that increased habituation. Imidacloprid, apomorphine, diphenhydramine, diazepam, and memantine decreased photomotor responses. Thus, the DPRA was also predictive in assessing the effects of neuroactive and neurotoxic environmental contaminants such as selective serotonin reuptake inhibitors, diazepam, organophosphorous, and neonicotinoid pesticides. We conclude that the proposed DPRA may be an effective screening tool for compounds that can impair predation avoidance behaviour in aquatic organisms.

Authors+Show Affiliations

Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Research Council (IDAEA, CSIC), Jordi Girona 18, 08034 Barcelona, Spain.Instituto Politecnico Nacional, Laboratorio de Hidrobiología Experimental, Escuela Nacional de Ciencias Biológicas, Mexico City, Mexico.Department of Analytical Chemistry and Applied (Chromatography section), School of Engineering, Institut Químic de Sarrià-Universitat Ramon Llull, Via Agusta 390, Barcelona 08017, Spain.Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Research Council (IDAEA, CSIC), Jordi Girona 18, 08034 Barcelona, Spain.Department of Analytical Chemistry and Applied (Chromatography section), School of Engineering, Institut Químic de Sarrià-Universitat Ramon Llull, Via Agusta 390, Barcelona 08017, Spain.Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Spanish Research Council (IDAEA, CSIC), Jordi Girona 18, 08034 Barcelona, Spain. Electronic address: cbmqam@cid.csic.es.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32559538

Citation

Bedrossiantz, Juliette, et al. "A High-throughput Assay for Screening Environmental Pollutants and Drugs Impairing Predator Avoidance in Daphnia Magna." The Science of the Total Environment, vol. 740, 2020, p. 140045.
Bedrossiantz J, Martínez-Jerónimo F, Bellot M, et al. A high-throughput assay for screening environmental pollutants and drugs impairing predator avoidance in Daphnia magna. Sci Total Environ. 2020;740:140045.
Bedrossiantz, J., Martínez-Jerónimo, F., Bellot, M., Raldua, D., Gómez-Canela, C., & Barata, C. (2020). A high-throughput assay for screening environmental pollutants and drugs impairing predator avoidance in Daphnia magna. The Science of the Total Environment, 740, 140045. https://doi.org/10.1016/j.scitotenv.2020.140045
Bedrossiantz J, et al. A High-throughput Assay for Screening Environmental Pollutants and Drugs Impairing Predator Avoidance in Daphnia Magna. Sci Total Environ. 2020 Jun 6;740:140045. PubMed PMID: 32559538.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A high-throughput assay for screening environmental pollutants and drugs impairing predator avoidance in Daphnia magna. AU - Bedrossiantz,Juliette, AU - Martínez-Jerónimo,Fernando, AU - Bellot,Marina, AU - Raldua,Demetrio, AU - Gómez-Canela,Cristian, AU - Barata,Carlos, Y1 - 2020/06/06/ PY - 2020/01/21/received PY - 2020/06/02/revised PY - 2020/06/05/accepted PY - 2020/6/20/pubmed PY - 2020/6/20/medline PY - 2020/6/20/entrez KW - Daphnia KW - Escape response KW - Habituation KW - Light KW - Neurotransmitters SP - 140045 EP - 140045 JF - The Science of the total environment JO - Sci. Total Environ. VL - 740 N2 - This study addresses short-term habituation of the escape response in the aquatic crustacean Daphnia magna evoked by sudden changes in light intensity, using a high-throughput system. Daphnia magna exhibits a marked phototactic behaviour and swim away from light to avoid predation by fish. Currently, there is no information available on the habituation of this phototactic response. The Daphnia photomotor response assay (DPRA) measures the distance moved after a sudden increase in light intensity. Using DPRA, it is possible to determine not only the magnitude of the phototactic response, but also its habituation after repetitive cycles of light and darkness. The progressive reduction observed in response to a series of light stimuli in the proposed assay meet the criteria for habituation. Most cholinergic and serotonergic modulators enhanced photomotor responses and reduced habituation. Dopaminergic and histaminergic modulators also reduced habituation, whereas diazepam was the only compound that increased habituation. Imidacloprid, apomorphine, diphenhydramine, diazepam, and memantine decreased photomotor responses. Thus, the DPRA was also predictive in assessing the effects of neuroactive and neurotoxic environmental contaminants such as selective serotonin reuptake inhibitors, diazepam, organophosphorous, and neonicotinoid pesticides. We conclude that the proposed DPRA may be an effective screening tool for compounds that can impair predation avoidance behaviour in aquatic organisms. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/32559538/A_high-throughput_assay_for_screening_environmental_pollutants_and_drugs_impairing_predator_avoidance_in_Daphnia_magna L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(20)33565-8 DB - PRIME DP - Unbound Medicine ER -
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