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Highly effective fibrin biopolymer scaffold for stem cells upgrading bone regeneration.
Materials (Basel). 2020 Jun 17; 13(12)M

Abstract

Fibrin scaffold fits as a provisional platform promoting cell migration and proliferation, angiogenesis, connective tissue formation and growth factors stimulation. We evaluated a unique heterologous fibrin biopolymer as scaffold to mesenchymal stem cells (MSCs) to treat a critical-size bone defect. Femurs of 27 rats were treated with fibrin biopolymer (FBP); FBP + MSCs; and FBP + MSC differentiated in bone lineage (MSC-D). Bone repair was evaluated 03, 21 and 42 days later by radiographic, histological and scanning electron microscopy (SEM) imaging. The FBP + MSC-D association was the most effective treatment, since newly formed Bone was more abundant and early matured in just 21 days. We concluded that FBP is an excellent scaffold for MSCs and also use of differentiated cells should be encouraged in regenerative therapy researches. The FBP ability to maintain viable MSCs at Bone defect site has modified inflammatory environment and accelerating their regeneration.

Authors+Show Affiliations

Center for the Study of Venoms and Venomous Animals (CEVAP), UNESP-São Paulo State University, Botucatu 18610-307, Brazil. Botucatu Medical School, UNESP-São Paulo State University, Botucatu 18618-687, Brazil.Center for the Study of Venoms and Venomous Animals (CEVAP), UNESP-São Paulo State University, Botucatu 18610-307, Brazil.College of Veterinary Medicine and Animal Husbandry (FMVZ), UNESP-São Paulo State University, Botucatu 18618-681, Brazil.Botucatu Biosciences Institute, UNESP-São Paulo State University, Botucatu 18618-689, Brazil.Botucatu Medical School, UNESP-São Paulo State University, Botucatu 18618-687, Brazil.Center for the Study of Venoms and Venomous Animals (CEVAP), UNESP-São Paulo State University, Botucatu 18610-307, Brazil. Botucatu Medical School, UNESP-São Paulo State University, Botucatu 18618-687, Brazil.Center for the Study of Venoms and Venomous Animals (CEVAP), UNESP-São Paulo State University, Botucatu 18610-307, Brazil. Botucatu Medical School, UNESP-São Paulo State University, Botucatu 18618-687, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32560388

Citation

Creste, Camila Fernanda Zorzella, et al. "Highly Effective Fibrin Biopolymer Scaffold for Stem Cells Upgrading Bone Regeneration." Materials (Basel, Switzerland), vol. 13, no. 12, 2020.
Creste CFZ, Orsi PR, Landim-Alvarenga FC, et al. Highly effective fibrin biopolymer scaffold for stem cells upgrading bone regeneration. Materials (Basel). 2020;13(12).
Creste, C. F. Z., Orsi, P. R., Landim-Alvarenga, F. C., Justulin, L. A., Golim, M. A., Barraviera, B., & Ferreira, R. S. (2020). Highly effective fibrin biopolymer scaffold for stem cells upgrading bone regeneration. Materials (Basel, Switzerland), 13(12). https://doi.org/10.3390/ma13122747
Creste CFZ, et al. Highly Effective Fibrin Biopolymer Scaffold for Stem Cells Upgrading Bone Regeneration. Materials (Basel). 2020 Jun 17;13(12) PubMed PMID: 32560388.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly effective fibrin biopolymer scaffold for stem cells upgrading bone regeneration. AU - Creste,Camila Fernanda Zorzella, AU - Orsi,Patrícia Rodrigues, AU - Landim-Alvarenga,Fernanda Cruz, AU - Justulin,Luis Antônio, AU - Golim,Marjorie de Assis, AU - Barraviera,Benedito, AU - Ferreira,Rui Seabra,Jr Y1 - 2020/06/17/ PY - 2020/05/11/received PY - 2020/05/29/revised PY - 2020/06/05/accepted PY - 2020/6/21/entrez PY - 2020/6/21/pubmed PY - 2020/6/21/medline KW - biomaterial KW - bioproduct KW - fibrin KW - scaffold KW - stem cell JF - Materials (Basel, Switzerland) JO - Materials (Basel) VL - 13 IS - 12 N2 - Fibrin scaffold fits as a provisional platform promoting cell migration and proliferation, angiogenesis, connective tissue formation and growth factors stimulation. We evaluated a unique heterologous fibrin biopolymer as scaffold to mesenchymal stem cells (MSCs) to treat a critical-size bone defect. Femurs of 27 rats were treated with fibrin biopolymer (FBP); FBP + MSCs; and FBP + MSC differentiated in bone lineage (MSC-D). Bone repair was evaluated 03, 21 and 42 days later by radiographic, histological and scanning electron microscopy (SEM) imaging. The FBP + MSC-D association was the most effective treatment, since newly formed Bone was more abundant and early matured in just 21 days. We concluded that FBP is an excellent scaffold for MSCs and also use of differentiated cells should be encouraged in regenerative therapy researches. The FBP ability to maintain viable MSCs at Bone defect site has modified inflammatory environment and accelerating their regeneration. SN - 1996-1944 UR - https://www.unboundmedicine.com/medline/citation/32560388/Highly_effective_fibrin_biopolymer_scaffold_for_stem_cells_upgrading_bone_regeneration L2 - https://www.mdpi.com/resolver?pii=ma13122747 DB - PRIME DP - Unbound Medicine ER -
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